Júlia Garcia-Baucells

This is a glimpse of the cytoskeletal structures we found doing U-ExM in more than 200 species, now in Cell🤩: shorturl.at/6oHvi
Amazing collaboration between @centriolelab.bsky.social , @dudinlab.bsky.social and @gautamdey.bsky.social labs.
I believe last one is a 🕷️
#FluorescenceFriday
#Microscopy

In vitro approaches to study centriole and cilium function in early mouse embryogenesis Although centrioles and primary cilia play an essential role in early mammalian development, their specific function during the interval between their initial formation and the subsequent arrest of em...

Really excited to see this out in the open: www.life-science-alliance.org/content/9/1/...! @isabellavoelkl.bsky.social explored and compared different in vitro developmental model systems to study ciliogenesis.

See also:

6/6 This was a huge team effort, and I'm deeply grateful to everyone involved: Carlo Bevilacqua & Robert Prevedel @prevedel-lab.bsky.social, Connie Rumpf-Kienzl, Alexandra Zampetaki & Sebastian Fürthauer, Manuel Rufin, Orestis G. Andriotis & Philipp J. Thurner!

5/6 2️⃣ Softening may allow the PCM to expand. This could promote microtubule nucleation throughout a larger volume, which is critical for building a large mitotic spindle quickly, especially in big embryonic cells. A mechanical adaptation for cell division!

4/6 Why would centrosomes need to soften? 1️⃣ Softer material dampens force fluctuations from the astral microtubules, which could protect chromosome attachments. A stiff centrosome wouldn't do this.

3/6 But are mitotic centrosomes more deformed just because they have more microtubules, or also because they get softer? We used two methods to find out: AFM on isolated centrosomes and Brillouin microscopy in living cells. The result? They soften during mitosis!

2/6 This deformation isn't from cortical pulling forces. It's caused by microtubules inside the centrosome scaffold. These internal microtubules define centrosome size, making it scale with cell volume. Deformation is smaller in smaller cells!


1/6 Extremely happy to share my PhD work is out on bioRxiv! We discovered that microtubules deform mitotic centrosomes. When we removed them, the SPD-5 scaffold became denser but didn't lose material. This suggests it's an elastically deformable solid! This only happens in mitosis.

6/end This was a huge team effort, and I'm deeply grateful to everyone involved: Carlo Bevilacqua & Robert Prevedel @prevedel-lab.bsky.social, Connie Rumpf-Kienzl, Alexandra Zampetaki & Sebastian Fürthauer, Manuel Rufin, Orestis G. Andriotis & Philipp J. Thurner!

5/n 2️⃣ Softening may allow the PCM to expand. This could promote microtubule nucleation throughout a larger volume, which is critical for building a large mitotic spindle quickly, especially in big embryonic cells. A mechanical adaptation for cell division!

4/n Why would centrosomes need to soften? 1️⃣ Softer material dampens force fluctuations from the astral microtubules, which could protect chromosome attachments. A stiff centrosome wouldn't do this.

3/n But are mitotic centrosomes more deformed just because they have more microtubules, or also because they get softer? We used two methods to find out: AFM on isolated centrosomes and Brillouin microscopy in living cells. The result? They soften during mitosis!

2/n This deformation isn't from cortical pulling forces. It's caused by microtubules inside the centrosome scaffold. These internal microtubules define centrosome size, making it scale with cell volume. Deformation is smaller in smaller cells!

Wohoo new paper from the lab! @shiviyaraina.bsky.social

Excited to share our discovery www.science.org/doi/10.1126/...

that the #centrosome is prone to breakage!

Using #motile cells, we identify centrosome breakage during #pathfinding, caused by #actin #forces, & prevented by #Dyrk3

Kudos to 1st author Madeleine & thanks to all collaborators!

(1/6)

With Microscopy Nodes, you can play with large volumes (this ExM dataset from Granita Lokaj is 49 GB total) and integrate this with all the cool tools in Blender, such as this #geonodes model of the centriole, made by @banterlegroup.bsky.social !

Our first paper on BlueSky!! Remarkable work published in @currentbiology.bsky.social from Jing Xie, Javad Najafi and others, on how cell shape affects mitotic spindle positioning forces in embryos, by impacting cytoplasm flows.

@ijmonod.bsky.social

www.sciencedirect.com/science/arti...

#TeamTomo visits the centrosome. I remember the centrosome and PCM being one of Julia's first targets when we were postdocs together at @mpibiochem.bsky.social. It's cool seeing the progress here in worms with Jeff. Beautiful #CryoET across the cell cycle. @centriolelab.bsky.social take a look! 🧪🧶🧬

Thank you Susana, Jadranka, Kevin and Jennifer for organizing these amazing sessions @ascbiology.bsky.social - so much exciting centrosome biology!!

what if organelle interactions could be created and controlled synthetically? if you are at ASCB tomorow, check out the session "Toward Building In Vitro Organelle Interaction Systems" Saturday 9:30- 11:00 room 33B organized by @marymirvis.bsky.social Hanaa Hariri, and Gant Luxton.

Excited to join the #CellBio2024 to present our novel investigations into the mechanical properties of the centrosome on Tuesday. It’ll be a pleasure to discuss the details of my project with you!

Congrats to Claudia Pachinger PhD (Dammermann Lab), who recently defended her thesis: Novel insights into centriolar satellites as regulators of centrosome and cilium function. Well done! @univie.ac.at @impvienna.bsky.social @imbavienna.bsky.social @gmivienna.bsky.social @maxperutzlabs.bsky.social