Apoorva Prabhu

Late arvo roboting = music concert for scientists ?

#eppendorf
#qpcr
#amplicon
#whyyyyyyyy

Slaying late workdays with cute lolliplots

Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses Our spatio-temporal metagenomic study of a subtropical estuary successfully identified viruses and their potential hosts. Viral contigs were detected using a consensus approach comprising geNOMAD and...

New #marine #archaea #virus paper alert!

Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...

co-authored with @chrisrinke.bsky.social @sjmcilroy.bsky.social , Julian Zaugg and Cheong Xin Chan

Machine learning and metagenomics identifies uncharacterized taxa inferred to drive biogeochemical cycles in a subtropical hypereutrophic estuary Abstract. Anthropogenic influences have drastically increased nutrient concentrations in many estuaries globally, and microbial communities have adapted to

Hope you had a fun read! This work was built on the previously characterised #Brisbane #River #Estuary, which is given here too :
academic.oup.com/ismecommun/a...

The genomes encoded phosphoesterase, which could be involved in membrane destabilisation. These #holin #endolysins systems need further investigations of course (culturing/transcriptomics), since mixed membranes of their Poseidoniales host that likely contain bacterial/eukaryotic G3P backbones.

The novel Poseidoniales virus, Adrikavirales become more interesting. This genome encoded which could be novel archeal-like #holins. Additionally, LytA-like (N-acetyl muramoyl L-alanine amidase) #endolysin was found adjacent to these holins, possibly aiding in viral #egress (see Fig 7).

These Poseidoniales viruses encoded a unique functional repertoire of genes. Aside from virion morphogenesis, and genome packaging and replication, and AMGs such as #biotin synthesis, #cobalamin productio and metallophosphoesterase, amidoligase, glutamine amidotransferase (GATase) among others.

In our study, we uncovered and proposed new names for clade A and E, for which we had complete viral genomes.

Two magrovirus families
Apasviridae (group A)
Krittikaviridae (group E)

….and a novel virus order Adrikavirales, which were also associated with #archaeal host order #Poseidoniales.

In this paper we explore the #viruses, particularly #metagenomic #archaeal #viruses of the #Brisbane #River #Estuary. Interestingly, viruses associated with archaeal host #Poseidoniales (which are grouped into magrovirusclade A-E and X), of which the name for clade B has been recently proposed.

Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses Our spatio-temporal metagenomic study of a subtropical estuary successfully identified viruses and their potential hosts. Viral contigs were detected using a consensus approach comprising geNOMAD and...

New #marine #archaea #virus paper alert!

Insights Into Phylogeny, Diversity and Functional Potential of Poseidoniales Viruses
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...

co-authored with @chrisrinke.bsky.social @sjmcilroy.bsky.social , Julian Zaugg and Cheong Xin Chan

Thanks, Chris! Writing this one was fun!

Quick, efficient, old school feel

Yes please! Microbial Ecology/Ecogenomics, working with metagenomics, viral genomics, and meta-transcriptomics data.

Would like to be added please! Working on improving water quality in estuaries and rivers

"I'm still a good boi" 😂

Thanks!!

Yayyy to virus host interactions. Can you include me please?

Thank you!

Thank you!!

Could I be added please? I use metagenomics to study prokaryotes and viruses in a subtropical estuary.

I'd love to be added! New to the field but working on virus metagenomics.

Could I get an add? Thanks!

Hi Jan, I'd like to be included- thanks!!

New to @bsky.app ! Marine / Estuarine microbial ecologist, researching microbes and viruses using omics methodologies.

Hi Steve! Could I get an add? Thanks!

Hi Steve, could I get an add? Thanks!

Hi Joe! Could I get added to this group please? Thanks!

I'd like to be added to the starter pack. Thank you