@luis-aj-alvarez.bsky.social
Physical Chemistry doctorate | Uni Paris - Orsay | Functional Imaging et al. | currently @ Leica Microsystems | opinions my own
Betty Yuen-Torres and Luis Alvarez holding the AACR SpectraPlex poster
Habemus Poster π #AACR2025
Just got the printout for our SpectraPlex Poster - it is proper size for the data we wanted to fit π. Come by tomorrow - section 7 board 24 poster 173 | happy to chat 3D high multiplexing imaging in cancer. Session starts at 14:00.
#AACR25 sign in Chicago for the annual American Association Of Cancer Reseaech conference
@ Chicago for #AACR2025 - if you are around and want to talk microscopy just drop me a message to meet up. Also we got a running STELLARIS with SpectraPlex at the @leicamicrosystems.bsky.social booth for 15+ channels on one go at the booth
26.04.2025 13:24 β π 2 π 0 π¬ 0 π 0
π£ The application deadline for #EMBLFluorescenceLifetime has been extended until 28 Apr! βπΌ
Are you a PhD student or a postdoc with experience in fluorescence microscopy and want to enhance your skills in confocal fluorescence microscopy and lifetime-based readouts? π¬
β‘οΈs.embl.org/lef25-01-bl
Cervical cancer tissue stained with PanCK-Opal 780 - Imaged on a Leica STELLARIS FALCON
Cervical Cancer tissue - PanCK Opal780 stained - left intensity only | right Fluorescence lifetime (rainbow 0,5-1,4 ns) showing the PanCK on cyan, non specific signal blue endogenous tissue signals in red #FluorescenceFriday #FluorescenceLifetime - interested ? FLIM course in July @ EMBL π
11.04.2025 19:44 β π 4 π 0 π¬ 0 π 0We have an amazing group of speakers and hands on sessions around Fluorescence Lifetime Imaging (multiphoton, metabolism, STED-FLIM, biosensing, Cryo, Plant imaging, multiplexing, FRET, functional dynamics and FLIM analysis.
Follow the link for program & application !
π£Application deadline for #EMBLFluorescenceLifetime is approaching β°
Do you have experience in fluorescence microscopy and want to enhance your skills in confocal fluorescence microscopy and in lifetime-based readouts?
Then apply for this hands-on course by 21 April: β‘οΈs.embl.org/lef25-01-bl
3D rendering of a Zebrafish embryo showing vasculature (GFP - magenta) and endogenous fluorescence (cyan) separated through Fluorescence Lifetime Imaging
3D rendering of a Zebrafish embryo showing vasculature (GFP) and endogenous fluorescence
#fluorescencefriday
#fluorescencelifetime
3D rendering Zebrafish embryo vasculature + endogenous fluorescence - intensity only (greyscale) corresponding lifetime separated (GFP - magenta | endogenous - cyan)
Interested? apply for our summer course @ EMBL in Heidelberg: www.embl.org/about/info/c...
2/2 hence encoding data is a 2 channel process in tiff. A workaround is to set it in HSV colour space with the brightness value set for intensity and playing on hue and saturation for the colour. I can provide raw images but it might require additional work to sort out LUTs.
13.01.2025 07:11 β π 1 π 0 π¬ 1 π 01/2 The lifetime bit is represented on a multicolour gradient. With the standard LUT being blue|cyan|green|yellow|red. It codes for intensity + lifetime or average arrival time (AAT) on a single channel. Values from 0 to 3/4 with 2 decimals (ns) or 3 digits (ps)
13.01.2025 06:36 β π 1 π 0 π¬ 1 π 0This has been my experience as well and for traditional 3-4 colour images it is always possible to find something fine when you hit 6+ and are integrating them I have not seen options that make sense - still keeping an eye out just in case π.
13.01.2025 06:29 β π 1 π 0 π¬ 0 π 0
For the classical fluorescence lifetime rainbow LUT please have a look at fig 1b here: www.nature.com/articles/d42...
12.01.2025 08:45 β π 1 π 0 π¬ 1 π 0
For the 15 colours please look at fig 3 a here : www.nature.com/articles/d42...
We tend to show subsets of markers at a time as well. Fig 3 b-g. But also fig 2 here www.nature.com/articles/d42...
Very nice ! We had some requests about colour blind alternatives for the βrainbowβ LUT used for FLIM or to get multiple FLIM channels. I could not find a solution. Now that we can get 15 markers at once I anticipate similar question for colour blind friendly set. Any ideas ? π
10.01.2025 06:28 β π 4 π 1 π¬ 2 π 0
Pithiviers pour galette des rois pour lβΓ©piphanie | Pithiviers puffy pastry French cake for wise man for January sixth
Part de galette des rois - Chocolat + frangipane filling
Γ chocolate & Frangipane filled Pithiviers for Galette des rois for Heilige Drei KΓΆnige | lβΓ©piphanie | best way to close the Christmas season ππ€©. I hope your end of the year a blast.
06.01.2025 23:00 β π 3 π 0 π¬ 0 π 0
Decorated and lighted up Christmas tree with gifts below
35 min past midnight and every thing is set for the kids to wake up early on Christmas Day π€£. Happy holidays to everyone and I hope you have a great time with family and friends!
24.12.2024 23:37 β π 0 π 0 π¬ 0 π 0Calling all #SciComm people who specialise in scientific animations. I would like to talk to someone about making some animations to communicate our research in single-molecule biophysics. Please message me if you are interested and share with your networks! π§ͺ
06.12.2024 10:50 β π 11 π 9 π¬ 3 π 0
15 colour beads - 0.7-0.9 ΞΌm | 405-850 nm | 50 X 75 ΞΌm image | xy view of a z stack
15 colour beads from 405 to 850 nm | side view of the z-stack with a line through the middle showing optimal chromatic correction across the complete spectra. All beads perfectly centered on the focal plane
15 colour beads 3D image | 2 ΞΌm z view showing all beads from 405 to 850 nm on the same z plane with close to isometric resolution
15 colours beads on a single slide π. Fluorescence emissions from 410 to 850 nm. 0.7-0.9 ΞΌm beads | z-stack showing the side view with proper chromatic correction across all wavelengths! Imaged on a STELLARIS with an extended white light laser (WLL) and SpectraPlex #FluorescenceFriday
06.12.2024 21:49 β π 11 π 1 π¬ 0 π 0How does it look like when calibrating it expresses in bacteria ? W/o purification ? & a calibration expresses in mammalian cells ?
29.11.2024 20:43 β π 2 π 0 π¬ 1 π 0
