cryo-em.bsky.social

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New preprint by @alok-bharadwaj.bsky.social explaining the workings of SURFER, a simple tool to segment membrane, micelle or nanodisc densities in cryoEM maps.

Try it via the @chimerax.ucsf.edu ToolShed:

cxtoolshed.rbvi.ucsf.edu/apps/chimera...

Despite LocScale in the name, it works on any map.

We’ve added our latest webinar to the product demos and technical presentations on our Videos page. Explore recordings covering our cameras, workflows, and real-world research applications.

Visit: https://directelectron.com/videos/

Question for the #cryoEM crowd: have you played with ModelAngelo, CryoAtom and EMProt, and would you care to offer thoughts/notes on the comparison? I have ModelAngelo installed and I love it, but the other two claim improved performance - which should I try first?

Since last year, I've been making a RELION GUI that supports viewing, navigating and running jobs in a way completely compatible with the original RELION pipeline. Now it's working nicely for both SPA and tomography! Please try it out - github.com/hanjinliu/hi...

Trimbody with rigid AI-designed scaffolds enables atomic-resolution cryo-EM structure determination of small proteins pubmed.ncbi.nlm.nih.gov/41735342/ #cryoEM

ChimeraX screenshot showing CubeNTube plugin.

The new ChimeraX CubeNTube plugin allows erasing parts of maps using cube, cylinder, and custom shapes and has undo. Created by Tamino Cairoli. Available from ChimeraX menu Tools / More Tools....

"QuickStainer": a rapid negative staining device for improved preservation of molecular structure pubmed.ncbi.nlm.nih.gov/41730344/ #cryoEM

Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies This article describes the transition of the Glass laboratory from X-ray crystallography to single-particle cryo-electron microscopy (cryo-EM) for structural studies of ATAD2B, a large AAA+ ATPase- and bromodomain-containing protein involved in chromatin regulation. It outlines practical strategies for protein expression, sample preparation, data processing and model building, offering a comprehensive workflow and troubleshooting guide for new cryo-EM users working with large, flexible macromolecular complexes.

Hassan Zafar et al.: Breaking barriers: transitioning from X-ray crystallography to cryo-EM for structural studies #CryoElectronMicroscopyCryoEM #StructuralBiology #SingleParticleAnalysis @uvmvermont...#IUCr https://journals.iucr.org/paper?S205979832600080X

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements Cryo-ET is a rapidly emerging technique that enables 3D visualization of complex biological structures, but present limits on signal-to-noise ratio and reconstruction quality pose challenges for downstream analysis. Here, we present a systematic analysis of state-of-the-art deep-learning methods for contrast enhancement and propose improvements in neural network architectures and training objectives to preserve more high-resolution information.

Henry N. Jones et al.: Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements #ContrastEnhancement ... #IUCr https://journals.iucr.org/paper?S2059798326001166

Cheat sheet for ChimeraX.

ChimeraX cheat sheet brought to you by Alejandro Pereira. www.rbvi.ucsf.edu/chimerax/dat...

Missed our recent webinar on the DE API for Direct Electron cameras and scan controllers? The recording is now on YouTube! Explore integration strategies and advanced workflow control with R&D Scientist Dr. Carter Francis. View the full recording: https://youtu.be/kJi1kLvHcGo

The amyloid packing difference (APD) is a new metric to compare amyloid structures. Analysis of disease-specific structures provides context for the interpretation of APDs for new comparisons. I hope it will be useful! 🤗

www.biorxiv.org/content/10.6...

The next #CryoEM Current Practices Webinar speaker is Vladimir Kolyadko from Children’s Hospital of Philadelphia speaking on "Cryo-EM of Liposome-Assembled Macromolecular Complexes to Reveal Protein-Membrane Interactions in Hemostasis" 2/26/2026 at 12PM ET / 9AM PT

us02web.zoom.us/webinar/regi...

Pulsed #lasers integrated with #cryoEM: @fitzpatricklab.bsky.social postdoc Daniel Du aims to boost contrast in #cryoET zuckermaninstitute.columbia.edu/making-world-s-best-microscopes-biology-better
zuckermaninstitute.columbia.edu/science-life-daniel-du
elifesciences.org/reviewed-preprints/109793v1

Please spread the word: the Structural Studies Division @mrclmb.bsky.social is looking for a new tenure-track, independent group leader with an exciting plan in any area of Structural (Molecular & Cell) biology, in discovery biology and/or methods development. 🥳

mrc.tal.net/vx/mobile-0/...

A gallium arsenide hybrid-pixel counting detector for 100 keV cryo-electron microscopy pubmed.ncbi.nlm.nih.gov/41680324/ #cryoEM

By 2028, all 4-character PDB IDs will be exhausted.

After that, all new entries will receive extended IDs: 12 characters total, formatted as pdb_ + 8 alphanumeric characters (pdb_1000axyz)

Test it from PDB Beta Archive

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Calling all microscopy enthusiasts! I have been working with Gail McConnell to coordinate the life-science section of the 21st International Microscopy Congress taking place in Liverpool, UK this September. We have some great symposia lined up!
Registration and abstracts 👉 www.imc21.org.uk

AlignPCA-2D: PCA-Reduced Euclidean Vector Alignment for 2D Classification in Cryo-EM https://www.biorxiv.org/content/10.64898/2026.02.11.705027v1

TANGO: Analysis and curation of particles in cryo-electron tomography pubmed.ncbi.nlm.nih.gov/41667456/ #cryoEM

SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI Jamaine Davis of Belmont University.… SBGrid Ecosystem

If you missed Alice-Roza Eruera's January webinar on Building CryoET Training Workflows Using the SBGrid Ecosystem, you can now find it on our YouTube channel.

Here: buff.ly/CPcy0Nz

#SBGrid #Webinars #ScienceMatters

Cryo-electron tomogram on a navy background with text: Cryo-ET Data Processing Workshop. June 7-11, 2026

Join us this summer for U-M's 2026 #CryoET Data Processing Workshop!
This four-day workshop will introduce participants to standard image processing packages and all aspects of cryo-ET data processing.
Apply by March 20: myumi.ch/lsi-cryo-workshop-2026

@jbquerido.bsky.social @shyamalm.bsky.social

Now available - Nanosoft FIB Autogrid Rings - The ring design offers major technical & usability improvements over other available FIB rings - For example significant increase in the area of your grid accessible for milling with the ion beam - Learn More zurl.co/hLTVf

Robust quality assessment of cryo-EM maps, tomograms and micrographs by statistics-based local resolution estimation https://www.biorxiv.org/content/10.64898/2026.02.03.703505v1

SBGrid Consortium For structural biologists, by structural biologists! The SBGrid TV channel was developed with participation from many members of the structural biology community. We welcome suggestions for topics to…

If you missed Alice-Roza Eruera's January webinar on Building CryoET Training Workflows Using the SBGrid Ecosystem, we will have the video posted to the SBGrid YouTube channel soon.

You can subscribe at buff.ly/RTDvlIb to receive alerts when new videos are added.

#SBGrid #Webinars #ScienceMatters

Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET pubmed.ncbi.nlm.nih.gov/41618010/ #cryoEM

good thread

Microsoft Virtual Events Powered by Teams Microsoft Virtual Events Powered by Teams

API WEBINAR - FEBRUARY 11th!
Learn how to use Python to control and automate Direct Electron cameras. Join R&D Scientist Carter Francis for a practical webinar covering live data visualization, experiment automation, Jupyter workflows, and new features.

Sign up here: https://ow.ly/cioN50Y5Jrl

Are you a cell biologist who secretly thinks cellular cryo-electron tomography is just George Palade-era EM, but fancier?

Join this webinar to see how modern #teamtomo has shifted from pretty pictures to quantitative, local membrane biology, and how that changes the questions we can ask.