puri fortes

The alternative initiation factor eIF2A regulates 40S subunit turnover in ribosome-associated quality control The noncanonical translation initiation factor eIF2A plays critical roles in diverse cellular processes, including the integrated stress response, neurodegeneration and tumorigenesis. However, the pre...

Identified in the 1970s, the precise molecular function of the alternative initiation factor eIF2A has remained unclear. Here, we map its interactome and uncover a surprising role in ribosome-associated quality control (RQC) upon ribosome stalling. 1/x

www.biorxiv.org/content/10.1...

An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation Cancer cells frequently enhance ribosome production to support rapid growth. Here, Lee et al. demonstrate that an isoform of lncRNA CRNDE containing an ultraconserved element (CRNDEUCE) promotes 60S r...

I had missed this super nice story!
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation: Molecular Cell www.cell.com/molecular-ce...

Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay Zhang et al. identify a regulatory mechanism for how cells adapt to nutrient scarcity through widespread −1 ribosomal frameshifts, culminating in accelerated mRNA decay. This process, dependent on codon optimality and conserved across species, establishes direct feedback coupling the translation of new proteins with the stability of the mRNA that encodes for them.

Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay

*LESS THAN 1 MONTH TO SUBMIT ABSTRACTS*

Sign up for the #GordonResearchConference on #microproteins and the #darkproteome now!

Scholarships are available for students. Apply by June 1 to be eligible!!!

www.grc.org/decoding-mic...

Single-molecule multimodal timing of in vivo mRNA synthesis mRNA synthesis requires extensive pre-mRNA maturation, the organisation of which remains unclear. Here, we directly sequence pre-mRNA without metabolic labelling or amplification to resolve transcript...

The timeline of co-transcriptional #mRNA processing and #modification may have to be revised: cleavage, termination, and #m6A deposition may occur earlier than most #splicing! Potential textbook changing study in bioRxiv by edueyras.bsky.social & Rippei Hayashi labs!
www.biorxiv.org/content/10.1...

I am happy to share the latest paper from the lab. We detail a tripartite cell-free translation system based on the separation of mRNAs, ribosomes and ribosome-depleted cytoplasmic lysate from human cells, allowing for flexible reconstitution of translation reaction.
#RNA #Ribosome

Deciphering disordered regions controlling mRNA decay in high-throughput - Nature Using high-throughput functional profiling, how disordered regions control mRNA stability and translation is explored.

Will be taking a deep dive into this today. Congrats to Joe Lobel and Nick Ingolia! #RNAsky #RNAbiology

www.nature.com/articles/s41...

Excited to announce a new paper out today in @cp-cell.bsky.social discovering RNA mis-splicing derived neoantigens & their cognate T cell receptors (TCRs) as well as characterization of endogenous neoantigen T cells in leukemia patients.

authors.elsevier.com/sd/article/S...