Ludwig Sinn

The "641 Hz" in the 750 Da/s-5 Da Q1-ZT Scan DIA method corresponds to the effective TOF-MS/MS scan rate after correcting for overheads from data binning, TOF-MS, and some other processes SCIEX knows more about

Hi Hamish,
to the best of my knowledge, "dwell time" here is defined as the time it takes to transfer ions of a certain m/z in the Q1. We tried to match ZT Scan's dwell time to ZenoSWATH's MS2 accumulation times for a fair comparison of both methods.
Indeed, 0.67 ms is the duration of a Zeno pulse.

Beyond support by SCIEX, we acknowledge funding from the BMBF, MSCoreSys, the SFB TRR186 and @erc.europa.eu.
A special Thanks to everyone involved from the Ralser Lab (ralser.group), SCIEX @sciex.bsky.social, as well as @vadim-demichev.bsky.social and @christophmessner.bsky.social.

We are super thrilled to see ZT Scan DIA leading to insight from miniscule proteome samples, in clinical proteomics and functional proteomics studies that depend on highly precise protein quantification from large sample series.

The meticulous development with our collaborators from SCIEX (@stevetate_absx and the magnificent team) was worth the effort, seeing gains between 30-40% in Protein IDs and improved quantitative performance in high-throughput, as well as with low-input proteomics applications.

Combining the benefits of ZenoTrap and Q1 scanning

Performance Characteristics of Zeno Trap Scanning DIA for Sensitive and Quantitative Proteomics at High Throughput Proteomic experiments, particularly those addressing dynamic proteome properties, time series, or genetic diversity, require the analysis of large sample numbers. Despite significant advancements in p...

I am excited to see our performance assessment of the successor of Scanning SWATH on the Zeno TOF7600+ mass spectrometer - ZT Scan DIA - pre-printed (www.biorxiv.org/content/10.1...