PhytoFrontiers™

FIGURE 1 Pipeline for obtaining species-specific sequences to Moniliophthora roreri (Mr). 1Genome MrNCBI of 57 Mb (Ecuador Mr-NCBI, ID 10809). 2 Other M. perniciosa (Mp) genomes: MpTc1441, MpSl178 (from CEPLAC/CEPEC-Bahia, Brazil), MpTc4145, and MpTcE404, INIAP/Ecuador. Cc, Ceratocystis cacaofunesta; Cr, Clonostachys rosea; Fs, Fusarium spp.; La, Lasiodiplodia spp.; Phyto, Phytophthora spp.; and Tc, Trichoderma spp.

Sara Pereira Menezes Reis et al. developed species-specific primers for biomonitoring frosty pod rot of #cacao. Learn more: https://doi.org/10.1094/PHYTOFR-03-24-0032-R

FIGURE 1 Growth of Ceratocystis fungal fruiting bodies (perithecia) on fine roots of Metrosideros polymorpha after artificial inoculation. Fine roots supporting the growth of mycelia and perithecia

“In Vitro Assays Suggest Rapid ‘Ōhi‘a Death Fungi Can Colonize Metrosideros polymorpha Fine Roots,” by Dylan Hyun et al. Read the article in Volume 5, Number 3 of PhytoFrontiers: https://doi.org/10.1094/PHYTOFR-01-25-0008-SC

FIGURE 8 Confocal laser scanning microscopy analysis showing Pseudomonas protegens

Using tomato as a model, Salomé Lengrand et al. designed a novel culture system to study the seed-borne endophytic bacterial community from seed to seedling, investigating its colonization and impact on plant health. Learn more: https://doi.org/10.1094/PHYTOFR-01-25-0004-R

FIGURE 2 Principal coordinate analysis (PCoA) of bacterial Bray-Curtis Dissimilarity by different management factors subset and compared either to or within only standard practice treatments. Bacterial communities were extracted and characterized from soils using amplicons produced from the 16S region (Bokulich and Mills 2013; Klasek et al. 2024).

Katherine A. Ashley et al. investigated the impacts of various management practices on soil microbial communities, including rotation length, non-grain rotation crops, green manures, compost amendments, and a soil fumigant using 2 potato varieties: https://doi.org/10.1094/PHYTOFR-02-25-0015-R

FIGURE 1 Maximum likelihood phylogenetic tree of selected complete PPV-D isolates. The tree is rooted on two M isolates (at the top of the tree). Lines connecting the D and M clades have been shortened to 10% of their original length to facilitate display on a single page. Each isolate is labeled with country and, where known, region of origin and GenBank number. Sequences from the United States are labeled in blue text, and isolates reported in this work are marked with red arrows.

Plum pox virus (PPV), a major threat to stone fruits, was eradicated from the U.S. after its 1999 discovery in Pennsylvania. Elizabeth E. Rogers, Andrew L. Stone, and Diana J. Sherman present genome sequences of 6 PPV finds from the eradication effort: https://doi.org/10.1094/PHYTOFR-09-24-0097-SC

On this #WorldFoodDay, we celebrate the efforts of those who nourish our communities, protect our planet, and shape a better future through better food for all. Explore resources to learn more about food security and sustainability. #FAO80

https://bit.ly/4nqN3x7

Effects of Preharvest Application of Acibenzolar-S-Methyl and Potassium Silicate on Postharvest Diseases and Fruit Quality of Blueberries | PhytoFrontiers™ Blueberries are highly perishable and susceptible to storage spoilage, especially fruit rot diseases caused by fungal pathogens. Alternaria rot caused by Alternaria alternata and gray mold caused by Botrytis cinerea are the primary postharvest diseases ...

Blueberries are highly susceptible to storage spoilage. Saiya Saito, Fei Wang, and Chang-Lin Xiao conducted a 3-year field trial to evaluate the effects of preharvest application of ASM and potassium silicate on Alternaria rot and gray mold in blueberry: https://doi.org/10.1094/PHYTOFR-10-24-0116-R

Efficacy of Five 14α-Demethylation Inhibitors and Four Biorational Fungicides Against Geotrichum candidum and Sour Rot of Peach | PhytoFrontiers™ Sour rot, caused by Geotrichum candidum Link (1809), is a postharvest disease of stone fruits, including peaches. The 14α-demethylase inhibitor (DMI) fungicide propiconazole has been used for postharvest sour rot management. However, the recent emergenc...

"Efficacy of Five 14α-Demethylation Inhibitors and Four Biorational Fungicides Against Geotrichum candidum and Sour Rot of Peach,” by Bingyu Zhao et al. 🍑 Read the article in Volume 5, Number 3 of PhytoFrontiers: https://doi.org/10.1094/PHYTOFR-12-24-0137-SC

Figure 1

Sensor-based techniques show potential as alternatives to visual rating techniques of plant diseases. Ronald Tapia et al. evaluated methods for canopy reflectance as a phenotyping approach that can be applied across many research contexts: https://doi.org/10.1094/PHYTOFR-06-24-0063-SC

FIGURE 1 The decision intelligence process flow has three phases: A, establishing requirements; B, mapping the decision; and C, assessing and improving the causal decision model. A and B comprise decision modeling, the process of reducing stakeholders’ knowledge and preferences to causal decision models (CDMs), and inserting artificial intelligence and other models into CDMs. B and C comprise decision reasoning, the process of using decision models to discover how actions lead to outcomes and to find better actions.

David L. Roberts et al. introduce decision intelligence to enhance nematode management in sweetpotato cultivation. 🪱🍠 Learn more: https://doi.org/10.1094/PHYTOFR-09-24-0099-P

FIGURE 1 The tails of A, Meloidogyne chitwoodi and B, Meloidogyne hapla (photos by Inga Zasada 2022).

Meloidogyne chitwoodi is one of the most important soilborne pests of potato. Michelle Soule et al. developed a qPCR assay for the identification and quantification of M. chitwoodi. Learn more: https://doi.org/10.1094/PHYTOFR-09-24-0107-FI

FIGURE 1 Overview of the MiFi pipeline for generating e-probes. First, complete or partially assembled genomes are aligned for target pathogen and near-neighbor candidates to produce raw e-probes. Then, raw e-probes are curated using BLAST against the entire NCBI database to eliminate potential cross-reactivity.

New EDNA e-probes developed by D. M. Nascimento et al. detect 20 citrus pathogens from raw HTS data. Validated as accurate and scalable tools, they support global citrus germplasm protection and biosecurity. https://doi.org/10.1094/PHYTOFR-12-24-0140-FI

Review of 25 studies applying the universal peptide nucleic acid (PNA) blockers developed by Lundberg et al. (2013) in 16S rRNA gene sequencing across different plant materials and organs reveals substantial variability in concentrations used

Lena Flörl and Nicholas A. Bokulich evaluated the efficacy of universal mitochondrial and chloroplast PNA blockers across a range of concentrations and estimated their impact on alpha and beta diversity. Learn more: doi.org/10.1094/PHYT...

Phylogenic tree inferred from the full-length sequence of the elongation factor 1-α gene (EF1-α) for Fusarium oxysporum f. sp. vasinfectum isolates. Included in the tree are isolates for which whole-genome sequences are available. The analysis was performed using sequences obtained from the NCBI GenBank. Sequences were aligned using the Clustal Omega alignment tool in Geneious Prime 2023.2.1 (https://www.geneious.com). Bayesian inference was performed using the MrBayes plugin in Geneious Prime 2023.2.1 using default settings and 1,100,000 bootstraps (Ronquist and Huelsenbeck 2003).

Timothy O. Jobe et al. screened #Fusarium oxysporum isolates for 14 “secreted in xylem” (SIX) effector genes—finding SIX9 shows potential for rapid detection and race-specific quantification in infected plants. Learn more: doi.org/10.1094/PHYT...

Signs and symptoms of drippy blight disease in Colorado and drippy nut disease in California. A, Symptomatic red oak branch displaying necrosis and oozing in Colorado; B, a common fly feeding off a dripping branch cutting in Colorado; C, symptomatic coast live oak displays necrotic acorn caps in California; D, symptomatic coast live oak acorn caps dripping with foamy bacterial ooze in California.

Editor’s Pick! Hope Raymond et al. compared the microbial communities of drippy blight in Colorado to those of drippy nut in California and to determine whether other pathogenic fungal or bacterial taxa are associated with the diseases. 🌳 Learn more: doi.org/10.1094/PHYT...

Dieback symptoms in Xylella fastidiosa-inoculated olive plants after regrowth. Inoculated plants were put through a vernalization process between 12 and 15 months postinoculation, after which new growth appeared. Dieback symptoms were still apparent in inoculated plants (Xf) as compared with healthy control plants (Ctl). Cultivar Mission was not included in comparisons at this point since several plants died of other causes.

Lindsey Burbank et al. evaluated several risk factors for Xyella fastidiosa disease in the California olive industry, including infection of the common cultivars and acquisition by insect vectors. 🫒 Learn more: doi.org/10.1094/PHYT...