Zunlong Ke

Prohibitins aren’t hanging out just anywhere in the membrane — they prefer the 💁‍♀️💎✨VIP section💅🪩🥂

Lab all-star @mmedina300kv.bsky.social maps these microdomains using cryo-ET + surface morphometrics.

s/o to #teamtomo #cryoET co-authors
@hamid13r.bsky.social
@attychang.bsky.social
@baradlab.com

Lupus Linked To Virus That Over 95 Percent Of Us Carry – And Now We Finally Know How "We think it applies to 100% of lupus cases."

IFL Science: “Lupus Linked To Virus That Over 95 Percent Of Us Carry – And Now We Finally Know How”

"We think it applies to 100% of lupus”
cases."

www.iflscience.com/lupus-linked...

MPI-WIS postdoc application Max-Planck–Weizmann Postdoc (4-year, co-mentored) Together with Ori Avinoam (Weizmann), we’re recruiting a postdoc to uncover membrane remodelling mechanisms in muscle development using recombinant pr...

MPI–Weizmann Postdoc (4-year, co-mentored)
Together with Ori Avinoam (Weizmann), we’re recruiting a postdoc to uncover membrane remodelling mechanisms in muscle development using cryo-EM of recombinant proteins, and cell imaging/CLEM. Apply here by 21.11.25 forms.gle/ZtDWUjcTBu4d...

Excited for Adam Klivans and my other colleagues at UT working in the AI space!

An Asgard archaeon with internal membrane compartments

Brilliant study led by @fmacleod.bsky.social and Andriko von Kügelgen. Tight collaboration with @buzzbaum.bsky.social and lab. Congrats to all authors!

www.biorxiv.org/content/10.1...

Glad to see our antibody design paper finally out in
@nature.com
(and congrats to lead authors and everyone involved )!
At Xaira we are excited about pushing antibody design further to bind harder targets and make drugs for unmet medical needs.
Paper:
www.nature.com/articles/s41...

Lab’s first paper is out!! We show the first structures of #Asgard #chromatin by #cryo-EM 🧬❄️
Asgard histones form closed and open hypernucleosomes. Closed are conserved across #Archaea, while open resemble eukaryotic H3–H4 octasomes and are Asgard-specific. More here: www.cell.com/molecular-ce...

Glushkova, Böhm, & Beck @maxplanck.de develop a publicly available computational method to measure the thickness of biological membranes in cryo-electron tomograms. Analysis of algae & human cells reveals systematic membrane thickness variations within & across organelles rupress.org/jcb/article/...

Systematic membrane thickness variation across cellular organelles revealed by cryo-ET pubmed.ncbi.nlm.nih.gov/41186573/ #cryoEM

Figure 1

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Lessons learned from a Kaggle challenge for particle picking in cryo-electron tomography [new]
Kaggle cryo-ET picking: Over-pick tol, aug, & annot key.

Just completed 100 miles at Austin rolling through Texas Hill Country. The event is 2025 LiveStrong Challenge to support cancer research. Feeling blessed!

Amazing looking!!!

We are looking for a computational postdoc to work with us on new optimisation algorithms to make #RELION even better. Join our bubbly team at the @mrclmb.bsky.social in Cambridge, UK. 🤗 RTs appreciated.

mrc.tal.net/vx/appcentre...

Composite image showing a pumpkin used for microscopy. A photo of the whole pumpkin with a small 2mm square cut out indicates the area imaged. The main background shows the orange flesh captured with a Keyence digital microscope, with the darker ridge on the left marking the skin. An inset black graph displays the Raman spectrum with strong beta-carotene peaks, explaining the pumpkin’s orange colour. Another inset image from cryo-SEM reveals tightly packed oval cells that make up the pumpkin’s fleshy interior. Images by Raymond Wightman and Daniel Sparrow.

Pumpkin on the Raman microscope

Flash frozen pumpkin in the vacuum chamber of the cryo-SEM before undergoing cryofracture and then coating in gold/palladium. It was then passed in to the cryo-SEM itself for imaging.

Ever seen a pumpkin under a microscope? 🎃🔬

This Halloween, Raymond Wightman & Daniel Sparrow used @slcuplants.bsky.social’s powerful imaging tools to reveal:
🔬 Skin detail with the #Keyence microscope
💡 Beta-carotene via #Raman spectroscopy
❄️ Flash-frozen oval cells via cryo-SEM

#HappyHalloween

Cryo-EM maps and atomic models of the biotin-containing 3-methylcrotonyl-CoA carboxylase (MCC) complex and long-chain acyl-CoA carboxylase (LCC) complex

New lab preprint!
@zestytoast.bsky.social tagged a scarce mycobacterial protein in M. smegmatis with TwinStep but got… something? @kjamali.bsky.social's ModelAngelo built models & @martinsteinegger.bsky.social's FoldSeek IDed them as the biotin-containing MCC & LCC complexes
🧵
tinyurl.com/ukny4ptz

The biophysics class I taught recently did kinesin walks hand-over-hand model (2004, Science), with reference to the inchworm model (2002,Science). Students discussed danced to mimic the two models, super fun!
Thanks to the shortage of giant squid due to meteorological disturbance El Niño in 1983!

My class recently did kinesin walks hand-over-hand model (2004, Science), with reference to the inchworm model (2002, Science)! Students danced to mimic the two models, super fun!

Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility Axoplasm from the squid giant axon contains a soluble protein translocator that induces movement of microtubules on glass, latex beads on microtubules…

Another undergraduate paper discussion today! This time we are doing Ron Vale's 1985 kinesin trifecta. Focusing on [1] & [3]. 🧪🔬

[1] www.sciencedirect.com/science/arti...
[2] www.sciencedirect.com/science/arti...
[3] www.sciencedirect.com/science/arti...
[4] www.ibiology.org/cell-biology...

Icecream: High-Fidelity Equivariant Cryo-Electron Tomography Cryo-electron tomography (cryo-ET) visualizes 3D cellular architecture in near-native states. Recent deep-learning methods (CryoCARE, IsoNet, DeepDeWedge, CryoLithe) improve denoising and artifact cor...

Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Excited to share a new chapter of my scientific journey in Chris Russo's group — developing a way to freeze proteins faster than they diffuse, paving the way for cryo-EM without air–water interface damage.

During these uncertain times, I’m very happy to see that my institution, @scripps.edu has an open tenure-track Assistant Professor position. Any field in Chemistry or Biology is welcome. I’d especially love to see fellow neuroscientists apply. Please repost!

apply.interfolio.com/174756

Assistant Professor in Structural Biology and Biochemistry, Department of Molecular Biology & Biochemistry University of California, Irvine is hiring. Apply now!

Our home department of Molecular Biology & Biochemistry at UC Irvine is looking to hire a new tenure-track assistant professor in the broad area of structural biology. Come be our colleague! recruit.ap.uci.edu/JPF09887

Please apply and/or share this post.

Hey folks! I got a tattoo of my beloved PIEZO ion channel on my arm! Now I can show how they activate (flatten) under pressure by flexing—Will add a bit of performance art to my science talks 🧪🧠.

🚀 Introducing fully automated data processing for repeat-target #cryoEM.

Using new tools in #CryoSPARC, it is now possible to obtain resolutions & map quality equal to or better than manual processing, with zero user intervention.

Preprint: www.biorxiv.org/content/10.1...

Yikes! In this paper the AlphaFold model of human plasminogen (PDB-8uq6) has been refined into noise (EMD-42462) using Phenix & ISOLDE. For some reason the authors even interpret side-chain interactions:
#cryoEM

Looks like apoB100 to me, especially the ordered CE core are quite the same

possibly stupid question - does anyone in #teamtomo or #cryoem recognize these little stacks (from EMPIAR-12549, U2OS cell lamellae)? The green circle is 300Å, and the spacing between the layers is ~50Å. The fourth, bell-shaped thing is something else, also not sure of that one..

It was wonderful to have Bijun with us @mpi-mp-potsdam.bsky.social & @incavirus.bsky.social to finally "use our microscope to its full potential" as our core-facility put it! 🔬

Congrats to all, esp. @bijuntang.bsky.social and @xanderjones.bsky.social ! Super happy to be a part of this great story!

I was tremendously lucky to be in @xanderjones.bsky.social's lab to watch @bijuntang.bsky.social move from technician to PhD to Postdoc and produce this magnum opus. #SalicS1 is the just reward for years of brilliance and hard work.Well done Bijun, Xander, Jing, and everyone involved! #SalicylicAcid