Rosy Favicchio

“I treated that access as something to be quiet about rather than discussed freely with others”

Peter Attia, explaining how to move in philanthropic circles and raise funds.

Desk reject.

🧪 #academicsky

Rooting for it! 🍀

Designing and testing CRISPRi-based synthetic gene circuits in plants Nature Protocols - This protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits...

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CRISPRi-based circuits can integrate different signals to achieve programmable gene expression in plants, while the high-throughput protoplast-based assay permits rapid and scalable testing of circuit components across different species.

The procedure covers engineered CRISPRi genetic circuits in plants, and outlines the isolation of protoplasts from different plant species and tissues, and for testing genetic parts and gene circuits using a plate-based, high-throughput, dual luciferase protoplast transfection assay.

Designing and testing CRISPRi-based synthetic gene circuits in plants - Nature Protocols This protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant spec...

Where is the #Arabidopsis community on @bsky.app? Any #Brassica fans? See our recent protocol covering design principles and considerations for building and testing programmable and reversible CRISPRi-based gene circuits in plants.
🧪 #plantscience @natprot.nature.com @ryanlister.bsky.social

Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas Nature Protocols - This protocol details transsynaptic tracing approaches using retrograde and anterograde viral tracers to map and manipulate neuron–tumor circuits in xenografts, brain...

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The procedure covers diverse experimental models to study neuron–GBM interactions, including the generation of GBO-cortical organoid assembloids, the transplantation of GBM cells into mouse brains and the co-cultures of GBOs with human primary brain tissue surgical slices.

This protocol describes rabies virus-mediated retrograde transsynaptic tracing and HSV-mediated anterograde transsynaptic tracing with GBM cells in xenografts and co-cultures, providing a platform for the systematic characterization of neuron–tumor connectomes.

Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas - Nature Protocols This protocol details transsynaptic tracing approaches using retrograde and anterograde viral tracers to map and manipulate neuron–tumor circuits in xenografts, brain organoid models and co-cultures.

A Protocol for characterizing the interactions between tumors and neurons in patient-derived GBM organoids:

🧪🧠 @natprot.nature.com

Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET Nature Protocols - A protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian...

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Montage parallel array cryo-electron tomography produces montages and individual tile tomograms, thereby eliminating possible mechanical adjustments to the transmission electron microscope.

Coupling 3D correlative focused ion beam milling with montage cryo-electron tomography enables the precise localization of regions of interest deep within cells and the acquisition of larger fields of view that preserve high-resolution structural details.

Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET - Nature Protocols A protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary ...

A workflow for 3D correlative cryo-fluorescence light microscopy-FIB-ET that streamlines fluorescence light microscopy-guided FIB milling, improving throughput while preserving both structural and contextual information
🧪🔬 #imaging @natprot.nature.com @erwright73.bsky.social

Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance Nature Protocols - A protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations...

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Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance - Nature Protocols A protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations required for designing multid...

Interested in magnetoencephalography? This protocol covers the planning of MEG studies, the execution of pilot experiments, the procedure implementation and the quality control required to ensure consistency in training and data acquisition and preprocessing.
🧠🧪 #neuroimaging @natprot.nature.com

A comprehensive framework for statistical testing of brain dynamics Nature Protocols - This protocol covers a package for running statistical testing on the temporal dynamics of neural activity data obtained from various functional neural recording modalities.

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A protocol on methods for testing statistical inference of brain dynamics and behavior. Variants of a hidden Markov model to characterize latent states in brain activity recorded from functional MRI, magnetoencephalography, electroencephalography, electrocorticography and local field potentials.

A comprehensive framework for statistical testing of brain dynamics - Nature Protocols This protocol covers a package for running statistical testing on the temporal dynamics of neural activity data obtained from various functional neural recording modalities.

Check out our recent protocol on a framework for statistical testing of brain dynamics based on an open-source Python package built on a generalization of the hidden Markov model (HMM).
🧪🧠 #neuroimaging @natprot.nature.com

Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles Nature Protocols - This protocol covers the acoustics-based separation of biological particles such as viruses and single extracellular vesicles from biofluids, including plasma and saliva, with...

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Acoustic separation and isolation of viruses, small extracellular vesicles and other nanoscale bioparticles - Nature Protocols This protocol covers the acoustics-based separation of biological particles such as viruses and single extracellular vesicles from biofluids, including plasma and saliva, with high purity.

A guide for designing and fabricating an acoustic separation device, including channel fabrication, the experimental setup and acoustic field optimization, as well as the biofluid preparation for the isolation of viruses, extracellular vesicles and other nanoparticles.

🧪 @natprot.nature.com

CellRank: consistent and data view agnostic fate mapping for single-cell genomics Nature Protocols - CellRank enables cell fate mapping from single-cell RNA sequencing data in a consistent and scalable manner, relying on different views of the data such as pseudotime or stemness...

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Cell–cell transition probabilities and terminal states can be inferred to study cell fate priming in the corresponding lineages. The approach incorporates complementary views of the data such as pseudotime, RNA velocity and experimental time points while facilitating newly emerging ones.

CellRank: consistent and data view agnostic fate mapping for single-cell genomics - Nature Protocols CellRank enables cell fate mapping from single-cell RNA sequencing data in a consistent and scalable manner, relying on different views of the data such as pseudotime or stemness potential, RNA veloci...

Did you catch our recent Protocol describing the inference of cell fate from single-cell RNA sequencing data using CellRank?

🧪 @natprot.nature.com @fabiantheis.bsky.social

and, in case you missed this recent news about AZ investing in China, here is their press release:

This post from a year ago has a good list of companies based in China, in case you are looking for one:

PS Dennis Gong doesn't seem to have a bsky account (but is @GongDennis on X)

China turns the tables in biotech For at least a century, major scientific research initiatives have reliably occurred in the United States, usually in response to geopolitical competition. This helped establish the country’s status a...

"all scientific knowledge is a public good, whatever its national origin" @holdenthorp.bsky.social writes about innovation in China's biotech, highlighting the need for cooperation between Eastern and Western hemispheres.

Scientific progress must rise above power politics.

In @natbiotech.nature.com a Comment discusses drug development in China which leverages the ability to undertake large-scale high quality cohort studies, sponsor-driven investigator-initiated trials, and the role of AI in speeding up precision medicine research.

🧪 1.5 years ago I asked whether the rise of research in China was going to influence the biomedical/healthcare sector, following similar success in other areas. Find below an update:

Multiplexed genetic tags for electron and fluorescence microscopy Nature Protocols - This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the...

Find the readcube link here: rdcu.be/eRElg