Eva-Maria Schentarra

Our article "Ångström-resolution imaging of cell-surface glycans" made it to the cover of Nature Nanotechnology! 🤩 @natnano.nature.com

www.nature.com/nnano/volume...

Localization of single molecules with structured illumination and structured detection - Light: Science & Applications Light: Science & Applications - Localization of single molecules with structured illumination and structured detection

Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field!

www.nature.com/articles/s41...

Mirror, mirror on the wall... 🪞 I'm super proud to have contributed to our latest work on left-handed DNA-PAINT! Here we introduce 6 new sequences using the left-handed enantiomers of our speed-optimized DNA-PAINT palette, enabling out-of-the-box 12-plex imaging without extra hybridization steps. 🌈🔬

Our new preprint is up! This is the main postdoc work of @wiesner-t.bsky.social focusing on exocytosis along the axon shaft and its regulation by the sub membrane actin-spectrin scaffold: www.biorxiv.org/content/10.1...
Read the thread below for a summary of our findings 🧵1/11

🚨 2 × PhD positions @EPFL! 🚨
Help us push the boundaries of fluorescence microscopy - DNA nanotech, custom optics & spatial omics in Lausanne 🇨🇭. Start Jan 2026. Send CV + motivation + 2 refs → fschueder@ethz.ch
#PhD #Hiring #microscopy #SuperResolution #SpatialOmics #DNAPAINT #FLASHPAINT

Congratulations to Ralf on your election as a new EMBO member:

❕Original press release from @embo.org : www.embo.org/press-releas...

Great science, great company and stunning views at our Lab retreat on Schloss Ringberg 🏰🧬🔬. Big thanks to our guests Sabrina Simoncelli, Sebastian Kobold, Thomas Schlichthärle, @massivephotonics.bsky.social & students from the @lfmilles.bsky.social and @mlsb-borgwardt.bsky.social Labs for joining!

Very excited to present our latest work: SPINNA, an analysis framework and software package for single-protein resolution data! 🖥️🤩

We can directly quantify stoichiometry and oligomerization from super-res (DNA-PAINT, RESI) images!! 🧬🎨

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution - Nature Communications Extracting quantitative information on biomolecular oligomerisation with high resolution remains a significant challenge. Here, the authors propose SPINNA, a framework that compares nearest-neighbour ...

Spatial and stoichiometric in situ analysis of biomolecular oligomerization at single-protein resolution

We are excited to present our latest work published in @natcomms.nature.com

www.nature.com/articles/s41...

Nanobinders for Synaptotagmin 1 enable the analysis of synaptic vesicle dynamics in rodent and human models. Synaptic neurotransmission is a critical hallmark of brain activity and one of the first processes to be affected in neural diseases. Monitoring this process, and in particular synaptic vesicle recycl...

Excited for our latest work! We developed nanobinders for the luminal domain of Synaptotagmin 1 (calcium sensor for synaptic vesicles). Thanks among others to @verstrekenlab.bsky.social @opazo.bsky.social @meunierlab.bsky.social @volkerhaucke-lab.bsky.social www.biorxiv.org/content/10.1...

IMAGING LIGAND-RECEPTOR INTERACTIONS AT SINGLE-PROTEIN RESOLUTION WITH DNA-PAINT🔬
Ever wonder how cells "talk"? It starts when ligands bind to receptors on cell surfaces. We have cracked the challenge of imaging small ligands on cell surfaces. #DNAPAINT #celltalk 💬
doi.org/10.1002/smtd...

Thanks to @eduardunterauer.bsky.social, @jekristina.bsky.social, @forna.bsky.social, @opazo.bsky.social and @carstenmarr.bsky.social for putting together this great step-by-step guide on how to achieve up to 30-color DNA-PAINT spatial proteomics at sub-15 nm resolution. 🚀

Cell Press: STAR Protocols STAR Protocols is an open access, peer-reviewed journal from Cell Press. We offer structured, transparent, accessible, and repeatable step-by-step experimental and computational protocols from all are...

One month ago today, I published my first paper with the @jungmannlab.bsky.social 🥳
What better way to celebrate #MicroscopyMonday than with this STAR Protocol on SUM-PAINT spatial proteomic imaging: a guide for highly multiplexed DNA-PAINT imaging in neurons. star-protocols.cell.com/protocols/4066

Polarity reversal of stable microtubules during neuronal development Neurons critically depend on long-distance transport orchestrated by motor proteins walking over their highly asymmetric microtubule cytoskeleton. These microtubules are organized uniformly in axons w...

My main PhD paper is on bioRxiv! 🥳 We used motor-PAINT, expansion microscopy, and live-cell imaging of StableMARK to map out how the microtubule cytoskeleton reorganizes during neuronal development.
www.biorxiv.org/content/10.1...

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

Whipped up a DNA-themed cake for the
@JungmannLab - sweet science at its finest! 🍰🔬 #PhDlife

Do you know what I love most about my PhD @JungmannLab? Actually seeing the things I learned from biology textbooks come to life🪄. Check out this DNA-PAINT image of the Golgi, showing both cis (GM130) and trans (TGN38) faces together with peroxisomes. Happy
#FluorescenceFriday😊

If you're curious about imaging more than 6 targets using DNA-PAINT, take a look at our recent publication on SUM-PAINT for high-throughput multiplexing

cell.com/cell/fulltext/…

Looking at this beautifully detailed neuron, stained for 9 different proteins using SUM-PAINT, feels like stepping into one of David S. Goodsell's paintings illustrating the crowded cellular environment. #FluorescenceFriday #phdlife @JungmannLab

Excited to attend my first ever conference on single molecule approaches to biology by @GordonConf in beautiful Maine!
Caught a fun 'Where's Waldo' moment in the hotel lobby - can you spot Ralf Jungmann and Luciano Masullo waving at me in the background? 🔍 @JungmannLab @l_masu

Happy Friday! To wrap up another exciting week of learning highly multiplexed DNA-PAINT in the @JungmannLab, here's a nice #cellfie of a rat hippocampal neuron stained for synaptic vesicle markers (Vamp2, VGlut1 and synaptotagmin), clathrin, neurofilament and peroxisomes.

With this my MINFLUX chapter may has concluded for now, but this was just the kick-off to my journey into super-resolution microscopy as a PhD student @JungmannLab 🚀

Here it is - fresh off the press. Tracking substeps of kinesin in mildly fixed neurons using MINFLUX 🔬.
I am proud to see my master thesis in close work together with Otto Wirth finally published!

nature.com/articles/s4200…

My first preprint as a joint first author together with Otto Wirth and I couldn't be more excited! 📚 🔬
Exploring kinesin with MINFLUX during my Master's thesis was a blast while working with such talented minds in the Hell Lab.

x.com/Stefan_W_Hell/… biorxiv.org/content/10.110…