Pacific Optica

Photo of Eccles. “Because [science] is the most effective way of learning about the physical world, it erodes superstition, ignorance and prejudice, which have been at the root of the denial of human rights throughout history…” -Baron Taverne

“Because [science] is the most effective way of learning about the physical world, it erodes superstition, ignorance and prejudice, which have been at the root of the denial of human rights throughout history…” -Baron Taverne

Photo of Rita Levi-Montalcini. "Because scientific knowledge is tentative and provisional, it is the enemy of dogma.” -Baron Taverne

“Because scientific knowledge is tentative and provisional, it is the enemy of dogma.” -Baron Taverne

Photo of Cajal. “Because science rejects claims to truth based on authority and depends on the criticism of established ideas, it is the enemy of autocracy.” -Baron Taverne

“Because science rejects claims to truth based on authority and depends on the criticism of established ideas, it is the enemy of autocracy.” -Baron Taverne

In 2025, we unlocked new frontiers in multiphoton optics and imaging.

In 2026, you can get the best 2p systems you’ve ever seen. Accelerating your work. Higher throughput. Easy to use. Focus on your neuro work, and leave the 2p to PacOp.

A person dressed up as a Cousa objective.

Happy Halloween!
pacificoptica.com/cousa/

Check out some sweet Ventana Wide + Cousa imaging.
pacificoptica.com/ventana/

Live raw data over a 3.2 mm wide field-of-view with 16,000 lines/s scanning and submicron resolution. Demo now at @omibs-mbl.bsky.social

Thx @ewadorotaz.bsky.social @mbfbioscience.bsky.social @crisrodtsou.bsky.social !

@pacificoptica.bsky.social @leicamicrosystems.bsky.social @thorlabs.bsky.social

Dover XYR and a Z elevator. dovermotion.com/xy-stages/#m...

Scientists explore, measure, gather evidence, figure things out, and share openly. Their work benefits us all.

Demo imaging at MBL/Woods Hole and CSHL this summer. Explore further. pacificoptica.com

Put whatever objective you want on the Ventana Wide. It will give you the largest field of view with high speed scanning: eg, 3.2mm x 3.2mm with a 10x. Fast, flexible 2p imaging.

Che-Hang Yu is helping MBL courses with this Ventana Wide system pacificoptica.com/ventana/

Just landed in Woods Hole for summer courses: a Full Ventana Wide system. pacificoptica.com/ventana/ with a Cousa pacificoptica.com/cousa/

www.mbl.edu/education/ad...

www.mbl.edu/education/ad...

Thanks, Coherent! Thanks, MBF! Thanks to course leadership and MBL staff!

Widespread cortical representations of innate behaviors in the mouse. www.biorxiv.org/content/10.1... Using the Diesel2p pacificoptica.com/diesel2p/, they found that "more directed and unilateral grooming movements had greater cortical associated responses than more
stereotyped bilateral movements"

We will start gathering at about 3:00.

吉祥寺 日曜日。2-photon, optics, 生ビルと　TexMex 食べ物。 maps.app.goo.gl/xoRrGNX7xvK1... ふるってご参加ください。

No more stitching artifacts. Just image the whole field-of-view, at high resolution, in one pass. The Ventana systems give submicron resolution over huge fields-of-view almost 10 square mm. At high speed. Cover a 3.2 mm x 3.2 mm area in 4k resolution in 0.26 s. pacificoptica.com/ventana/

Explore further. pacificoptica.com/diesel2p/

Cool!! This is an exciting new Neuro AI #NeurIPS competition.

By the way, the large scale 2p data for this NeurIPS competition was taken with a Diesel2p system. pacificoptica.com/diesel2p/

🌌 Curious how visionary engineering shaped one of humanity’s greatest scientific instruments? Discover the architectural origins of the JWST in this in-depth #SPIE_JATIS article: www.spiedigitallibra...

YouTube video by Spencer LaVere Smith Non-inertial scan angle multiplier - AI-generated podcast with actual human commentary

ALERT: New journal club style!

S. L. Smith listens to and provides commentary on an AI-generated fake podcast on their new preprint.

Hilarious in parts, and fun and chill.

Everyone: Please do this for your papers too!
m.youtube.com/watch?v=qO1n...

So excited to hang the ‘Acknowlegement Guidelines’ poster up in Chapel Hill! Grateful to be part of the community working towards equitable recognition for imaging scientists!!

Read more and download the poster (in 10 languages!): lnkd.in/eSQRugJX

#CreditWhereItsDue
#AcknowledgementGuidelines

Now published in Plos Biology journals.plos.org/plosbiology/...

Animals sense in complex environments, and neuronal responses to olfactory/scent stimuli can be shaped by recent history going back ~ 30 s. Here, Subramanian, Storace, et al. used the Cousa objective pacificoptica.com/cousa/ to neural activity in vivo. doi.org/10.1093/chem... #microscopymonday

Multinucleated giant cells (MNGCs) emerge as ovaries age, and are hard to study. Here, Converse, Pritchard, Duncan et al. used 2p imaging with the Cousa objective pacificoptica.com/cousa/ in their comprehensive study of age-associated MNGCs. #microscopymonday www.biorxiv.org/content/10.1...

This system has tons of fascinating tech— optics, detectors, software— so many scientists and engineers collaborated on this international project, enjoy the fruits of their talents!

Thanks, Bruno! ♥️

Fantastic work (as always) from Che-Hang Yu and colleagues in Spencer LaVere Smith's lab. Very neat method to increase scan speed and/or FOV in 2p microscopy. Commercially available from @pacificoptica.bsky.social. I'd love to build this for a client at some point! www.biorxiv.org/content/10.1...

Rubin Observatory's car-sized digital camera mounted on the telescope. Seen at an angle face-on, the camera is a long black tube cutting vertically through the top two-thirds of the image. Its giant clear glass lens is visible toward the top. To the left and right of the camera, scaffolding for staff access is set up. The background is an array of black and teal steel, both part of the telescope and beyond as part of the dome.

🚨THIS IS NOT A DRILL🚨We are 6 days away from the world's First Look at images from Rubin! #RubinFirstLook 🔭🧪

NSF–DOE Rubin Observatory will take a new 3200-megapixel image every ~40s, yielding ~20 terabytes of data every night! The deluge of data is about to begin 🌊

YouTube video by Spencer LaVere Smith Non-inertial scan angle multiplier - AI-generated podcast with actual human commentary

ALERT: New journal club style!

S. L. Smith listens to and provides commentary on an AI-generated fake podcast on their new preprint.

Hilarious in parts, and fun and chill.

Everyone: Please do this for your papers too!
m.youtube.com/watch?v=qO1n...

So the optical invariant for scanning is not so invariant!

Here's a link to the preprint. www.biorxiv.org/content/10.1... Be sure to check out the videos online. www.biorxiv.org/content/10.1... Raw data videos included. 9/9

There is a bunch more in the paper, including this variation for doubling the phase modulation off of an adaptive optic element. Limited range on your deformable mirror? Not a problem anymore. 8/9