bioRxiv Biochemistry

Public Cohort Analysis Identifies Thyroglobulin Variants as Hypothyroidism Risk Factors https://www.biorxiv.org/content/10.1101/2025.11.21.689591v1

Coordinated regulation of the metaboproteome by Hsp90 chaperones controls metabolic plasticity https://www.biorxiv.org/content/10.1101/2025.11.21.689798v1

Fluorescent Biosensor-Guided Engineering of Enzyme Cascades for Electrochemical Applications https://www.biorxiv.org/content/10.1101/2025.11.21.689824v1

2D HSQC-derived "dark forest" image with enhanced local resolution via first derivative processing-logarithmic cosine transformation (FDP-LCT): Demonstration on per-O-ethylated kappa- and iota-carrageenans https://www.biorxiv.org/content/10.1101/2025.11.21.689839v1

Systematic characterization of cancer-associated SPOP mutants reveals novel and reprogrammable degradative activities https://www.biorxiv.org/content/10.1101/2025.11.21.689851v1

Early Host-Virus RNA Interactions Reveal SPEN-Driven m6A Regulation as a Major Determinant of Henipavirus Infection https://www.biorxiv.org/content/10.1101/2025.11.21.689838v1

Engineered Transdermal Peptide-Recombinant Type III Collagen Hydrogel with Biological Efficacy in Cell Proliferation and Wound Healing https://www.biorxiv.org/content/10.1101/2025.11.22.689886v1

Alirocumab attenuated plaque inflammation and PCSK9-induced proinflammatory signalling in M1 macrophages independently of lipid lowering https://www.biorxiv.org/content/10.1101/2025.11.20.689631v1

Genetic variation of human G6PD impacts Red Blood Cell transfusion efficacy https://www.biorxiv.org/content/10.1101/2025.11.21.689741v1

Computational design of cysteine proteases https://www.biorxiv.org/content/10.1101/2025.11.21.689808v1

Rapid aging and disassembly of actin filaments from two evolutionary distant yeasts https://www.biorxiv.org/content/10.1101/2025.11.21.689671v1

Design and analysis of synthetic carbon fixation pathways based on novel enzymatic reactions https://www.biorxiv.org/content/10.1101/2025.11.21.689712v1

Structural and energetic insights into human rhomboid proteases reveal a unique lateral gating mechanism for orphan family members https://www.biorxiv.org/content/10.1101/2025.11.21.689725v1

ApoA-I dissociated from human HDL retains its acceptor properties in ABCA1-mediated cholesterol efflux from RAW 264.7 macrophages in coronary artery disease https://www.biorxiv.org/content/10.1101/2025.11.21.689699v1

A stress-dependent TRIM28-ALKBH2 feedback loop modulates chemotherapy response https://www.biorxiv.org/content/10.1101/2025.11.21.689678v1

Computational design of metalloproteases https://www.biorxiv.org/content/10.1101/2025.11.20.689622v1

NUDT5 regulates the global efficacy of nucleoside analog drugs by coordinating purine synthesis and PRPP allocation https://www.biorxiv.org/content/10.1101/2025.11.20.689348v1

The trimeric Shu complex in C. elegans is an ATPase that remodels RAD51 filaments in the homologous recombination-associated DNA damage response. https://www.biorxiv.org/content/10.1101/2025.11.17.685403v1

De Novo Design of a Protein Binder to Probe Gas Channel and Enhance the Oxygen Tolerance of [NiFe] Hydrogenase https://www.biorxiv.org/content/10.1101/2025.11.19.689374v1

Cycling Molecular Assemblies (CyMA) for Ultrasensitive Golgi Imaging https://www.biorxiv.org/content/10.1101/2025.11.19.689330v1

Fix or Freeze? Spectral Differences Arising from Tissue Preparation in Chemical Imaging https://www.biorxiv.org/content/10.1101/2025.11.19.689284v1

Linker-rigidified VHL homodimerizers convert degraders into stabilizers of non-ubiquitinable ternary complexes https://www.biorxiv.org/content/10.1101/2025.11.18.689129v1

A nucleic acid labeling chemistry reveals surface DNA on exosomes https://www.biorxiv.org/content/10.1101/2025.11.18.689134v1

Charting the Undiscovered Metabolome with Synthetic Multiplexing https://www.biorxiv.org/content/10.1101/2025.11.18.689170v1

Biosynthesis of peptidic thiooxazole metallophores installed by multinuclear nonheme iron enzymes. https://www.biorxiv.org/content/10.1101/2025.11.18.689057v1

The electrophilic metabolite of kynurenine, kynurenine-CKA, targets C151 in Keap1 to derepress Nrf2. https://www.biorxiv.org/content/10.1101/2025.11.18.689077v1

High-throughput functional profiling and evolutionary covariation analysis of entire riboswitch sequences https://www.biorxiv.org/content/10.1101/2025.11.18.689055v1

Pho-Tip: one-pot dephosphorylation for rapid and sensitive analysis of DIA phosphoproteomics data https://www.biorxiv.org/content/10.1101/2025.11.17.687597v1

A universal buffer system for native LC-MS analysis of antibody-based therapeutics https://www.biorxiv.org/content/10.1101/2025.11.17.688837v1

Induced ubiquitination of the partially disordered Estrogen Receptor alpha protein via a 14-3-3-directed molecular glue-based PROTAC design https://www.biorxiv.org/content/10.1101/2025.11.17.688825v1