bioRxiv Biochemistry

Thymine DNA Glycosylase Binds to R-Loops and Excises 5-Formyl and 5-Carboxyl Cytosine from DNA/RNA Hybrids https://www.biorxiv.org/content/10.1101/2025.08.05.668694v1

Characterization of bacterial fucokinase/GDP-fucose pyrophosphorylase (FKP) enzymes supports the evolution of interdomain communication and modularity in the FKP family https://www.biorxiv.org/content/10.1101/2025.08.05.668714v1

Live-cell imaging and lipidomics of low density lipoprotein containing intrinsically fluorescent cholesteryl esters https://www.biorxiv.org/content/10.1101/2025.08.05.668602v1

Structural mechanisms for the recruitment of factor H by Streptococcus pyogenes https://www.biorxiv.org/content/10.1101/2025.08.05.668778v1

Structural and dynamic insights into phosphate uptake by PHT1 transporter in rice https://www.biorxiv.org/content/10.1101/2025.08.04.668567v1

Purification of amylase from local isolate Bacillus subtilis A4 https://www.biorxiv.org/content/10.1101/2025.08.04.668472v1

In-cell structure of a LINC complex reveals the molecular basis for membrane remodelling and head-to-tail coupling in sperm cells https://www.biorxiv.org/content/10.1101/2025.08.04.668116v1

Dual E3 ligase recruitment by monovalent degraders enables redundant and tuneable degradation of SMARCA2/4 https://www.biorxiv.org/content/10.1101/2025.08.04.668513v1

Cardiac metabolite exchange measured in pigs during myocardial ischemia and reperfusion https://www.biorxiv.org/content/10.1101/2025.08.04.668556v1

DRUMBEAT: Temporally resolved interpretable machine learning model for characterizing state transitions in protein dynamics https://www.biorxiv.org/content/10.1101/2025.08.04.668534v1

Covalent Degraders of Immune Regulatory Transcription Factors IRF8 and IRF5 https://www.biorxiv.org/content/10.1101/2025.08.03.668300v1

Design of Orthogonal Far-Red, Orange and Green Fluorophore-binding Proteins for Multiplex Imaging https://www.biorxiv.org/content/10.1101/2025.08.03.668343v1

Quantitative Membrane Binding Assays Reveal an Inhibitory Role for the BRAF-Specific Region in CRD and Lipid Interaction https://www.biorxiv.org/content/10.1101/2025.08.03.668294v1

A combinatorial mutational map of active non-native protein kinases by deep learning guided sequence design https://www.biorxiv.org/content/10.1101/2025.08.03.668353v1

Dynamic allostery drives acetyl-CoA-mediated activation of Mycobacterium tuberculosis isocitrate lyase 2 https://www.biorxiv.org/content/10.1101/2025.08.02.651367v1

The structure of Egalitarian in complex with the K10 mRNA localization signal reveals a modular binding surface required for function https://www.biorxiv.org/content/10.1101/2025.08.03.668247v1

Selectivity of the time-dependent M. tuberculosis LeuRS inhibitor ganfeborole is driven by target vulnerability https://www.biorxiv.org/content/10.1101/2025.07.31.667927v1

Post-Translational Modifications Remodel Proteome-Wide Ligandability https://www.biorxiv.org/content/10.1101/2025.07.31.667978v1

RPA directly stimulates Mer3/HFM1 helicase processivity to ensure normal crossover formation in meiosis https://www.biorxiv.org/content/10.1101/2025.08.02.668043v1

Structural framework for the assembly of the human tRNA ligase complex https://www.biorxiv.org/content/10.1101/2025.08.01.668197v1

Vacuolar Phosphatidylinositol 3,4,5-trisphosphate controls fusion through binding Vam7, and membrane microdomain assembly https://www.biorxiv.org/content/10.1101/2025.08.01.668199v1

Structure-Guided Design of a Bioactive Covalent Small Molecule Targeting a Riboswitch https://www.biorxiv.org/content/10.1101/2025.08.01.668122v1

Efficient spatial gene expression profiling using split-probe ligation and rolling circle amplification https://www.biorxiv.org/content/10.1101/2025.08.01.668044v1

Structural and mechanistic insights into Dis3L2 mediated degradation of structured RNA https://www.biorxiv.org/content/10.1101/2025.08.01.668158v1

Temporal dynamics of RNA metabolic enzyme interactome lay the foundation for riboregulation mediated circadian metabolism https://www.biorxiv.org/content/10.1101/2025.07.31.667909v1

Structures of Naked Mole-Rat, Tuco-Tuco, and Guinea Pig Ribosomes: Is rRNA Fragmentation Linked to Translational Fidelity? https://www.biorxiv.org/content/10.1101/2025.08.01.667930v1

Enzyme-free biochemical production of seamlessly N-to-C cyclized peptides from natural or recombinant proteins https://www.biorxiv.org/content/10.1101/2025.07.31.667801v1

The competitive interplay of 12-oxophytodienoic acid (OPDA), protein thiols and glutathione https://www.biorxiv.org/content/10.1101/2025.07.31.667886v1

Definitive benchmarking of DDA and DIA for host cell protein analysis on the Orbitrap Astral in a regulatory-aligned framework https://www.biorxiv.org/content/10.1101/2025.07.31.667876v1

Specialization and adaptation in pollen sterol use by wild bees https://www.biorxiv.org/content/10.1101/2025.07.31.667884v1