@saqlainh.bsky.social
PhD. candidate, Farrona lab, University of Galway
We are super proud of Nora @norapasqualimedici.bsky.social and her poster award at the College of Science & Engineering Research & Innovation Day last week ๐คฉ๐ฑ๐ฅณ @episeedlink.bsky.social @uniofgalway.bsky.social
16.05.2025 09:16 โ ๐ 3 ๐ 1 ๐ฌ 0 ๐ 1
Two text bubbles in yellow and blue on the left and the Node logo on the right. Text reads "Honest conversations: creating frank conversations around less-discussed topics on the Node"
Our 'Honest conversations' series is a place for people to write about the ups and downs in their academic journey and create frank conversations around less-discussed topics.
Read the posts, keep the conversations going, and perhaps write a post yourself:
thenode.biologists.com/honest-conve...
Arabidopsis INHIBITOR OF GROWTH 2 (ING2) promotes flowering by regulating NuA4-dependent histone H4 acetylation levels at FT and SOC1 chromatin loci https://www.biorxiv.org/content/10.1101/2025.03.10.642062v1
13.03.2025 14:03 โ ๐ 0 ๐ 2 ๐ฌ 0 ๐ 0(7/n)
Overall, this is a first study of its kind that explores the PWO-PRC2 interaction and how PWOs might have contributed to PRC2 evolution in vascular plants.
(6/n)
Finally, we introduced SmPWOs (SmPWOa and SmPWOb) in At pwo1, pwo2 mutants as a way to analyze if representative of ancestral PWOs could complement the defects in At. Interestingly, SmPWOa could partially complement all the defects observed in At pwo1:pwo2 mutants.
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Further, PWOs from At and Sm could interact with moss PRC2 components, despite the absence of PWOs in Physcomitrella patens. Next, a short C-terminal motif was identified in PWOs, which is proposed to provide/ contribute to an interaction interface for PWO-PRC2 binding.
(4/n)
As a representative of ancestral PWOs, we worked on Selaginella moellendorffii (Sm) PWOs (SmPWOa/b) and showed their interaction with Arabidopsis thaliana (At) PRC2 catalytic subunits (SWN/CLF).
(3/n)
PWOs have been proposed to play a role in transcriptional activation as well as repression through their association with PEAT and PRC2 complexes. To better understand the PWO-PRC2 interaction, a range of PPI studies were conducted, including Y2H, BiFC, and FLIM-FRET.
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Unlike PRC2 components that are present in all plants, PWOs are absent in lower plants (Green algae and Bryophytes) and first emerged in vascular plants, i.e., Lycophytes (Selaginella moellendorffii), coinciding with the transition to land
New Preprint Alert
This work explores the origin of PWO proteins in plants and their association with PRC2 components throughout evolution.
(1/n)
Photo of a gloved hand holding a pcr tube strip with 20uL of clear liquid in each in a biosafety cabinet.
To me, this is incredibly beautiful and wonderous. Such clean, small liquids are holding a plethora of molecules I leveraged nature to create exactly as I want them. Picturing it in my mind then making them in these invisible liquids is just incredible to me. This is why I'm a molecular biologist.
21.03.2025 21:17 โ ๐ 364 ๐ 22 ๐ฌ 15 ๐ 2PWO proteins are associated with PRC2 since their emergence in vascular plants https://www.biorxiv.org/content/10.1101/2025.03.15.643013v1
17.03.2025 21:01 โ ๐ 6 ๐ 4 ๐ฌ 0 ๐ 1
