Ask Zebrafish?

There's still some left of the 3rd edition, for those interested! These are handcut linographs not prints that are sure to impress 😍 #ZebrafishGear

🔗: www.thewildprintstudio.com/shop/p/zebra...

Black sheet with nine Danio species on it including zebrafish and Giant danios

Single drawing postcards of danio species. The the top right is Danio rerio

Give a unique fishy gift with these Danio drawings! Made by @rfallax on X. Available in groups or single fish. Many other types of fish types on website: osakanacraft.thebase.in #ZebrafishGear

Two adult zebrafish necklaces in gold and silver. Made by Nikola Jewelry on Etsy.

The perfect gift for the #zebrafish fanatic in your life! Designed by Nikola Jewelry on Etsy. Available in multiple metals. To purchase, visit www.etsy.com/uk/listing/1... #WhyAmIOnEtsy #ZebrafishGear

Image of a white coffee mug with the text 'MAY THE EMBRYOS BE EVER IN YOUR FAVOR'

A gift for the embryologist in your life 🥚 t.co/InkPoS26Nf #ZebrafishGear #WhyAmIOnEtsy

"Twelve danios" design. The common names of these fish are: Zebra danio, Meghalaya danio, spotted danio, gold-ring danio, celestial pearl danio, emerald dwarf rasbora*, glowlight danio, moustached danio, rosy danio, pearl danio, blue danio, and yoma danio. * Despite the name, it is still a member of the Danio genus! Credit text to rah-bop (Redbubble)

We may be pro-rerio, but we're pro-Danio, too! To purchase this design on various media, visit: redbubble.com/i/postcard/T... #ZebrafishGear

🤔 #AskZebrafish: "We are interested in doing some heart explants from zebrafish at 24 hpf as described in Noël ES, et al PMID: 24212328 (pubmed.ncbi.nlm.nih.gov/24212328/). Has anyone had experience doing this that might be able to help my student become proficient? Thanks!"

Q: What do you call two #zebrafish destined for each other? #ZebrafishJokes

A: Shoalmates 🥰

🤔 #AskZebrafish from Slack: Looking for a supplier of hatchfry encapsulon and microfine spirulina that has good customer service. Preferably available in the US.

👀 #AskZebrafish: Some members of the community are looking for a few plasmids. If you have any of the following and willing to share, please reach out 🙏

mNeonGreen
VenusPEST
gfap and ptf1a promoters

🤔 #AskZebrafish: Does anyone have experience extracting (tissue-specific) cell monolayers for culture from ZF embryos? Looking for a contact to pick their brain.

🤔 #AskZebrafish: "Do any of you have a protocol or a kit recommendation to do subcellular fractionation of zebrafish embryos. Ideally I'd like to separate the mitochondria and the nucleus (and as a bonus the rest of the cell parts)"

Thanks for the reply 🙏 We’ll ask them and circle back.

🤔 #AskZebrafish: “Wondering if anyone has recommendations on a relatively inexpensive device to measure water flow rates in a swim tunnel?”

#AskZebrafish from Slack: What is the best promoter to use to specifically tag microglia in #zebrafish? Thinking of using mpeg1.1 but just wanted to double check if there are any better options?

Query from Slack: Looking for Tg(pax3:GAL4) and any driver line for ascl1:GAL4 or Cre? Preferably in Europe. Any help and/or leads would be appreciated! #AskZebrafish

🤔 #AskZebrafish: Looking for a zebrafish version of the Mito-RiboTag mouse line. Has anyone made one or might have a vector they could share? Any leads would be thoroughly appreciated!

We got a bite on our Slack channel. Appreciate your help!

Question from Slack: Looking for antibodies against mscarlet-i that people have good experience with? #AskZebrafish

Query from Slack: Looking for notch2 mutant zebrafish and someone willing to share them? Preferably in the US. 🔍 #AskZebrafish

#AskZebrafish query from Slack: Looking for contact that has recently shipped transgenic eggs from Europe to the US. Re: any hurdles you or your lab had to go through. If this describes you or someone you know, please reach out 🙏

Initial trawl came up empty 🤷‍♂️ Any leads or feedback appreciated!

Query from Slack: “I was wondering if anyone has a spi1b (pu.1) HCR probe that looks good in whole mount [zebrafish] tissues, the one I designed has a lot of pesky background in the embryo.” #AskZebrafish

Query from Slack: Has anyone successfully used a c-fos antibody for whole mount immuostaining in zebrafish? #AskZebrafish 🤔

Two queries from Slack:

Anyone know of any good antibodies that work well in whole mount 5-7dpf for labeling mitochondria?

Does anyone in the community work with mitochondria based assays for larval zebrafish? Such as Alamar blue or JC10? Or anything else?

Any leads appreciated!

#AskZebrafish

🤔 #AskZebrafish from Slack: "Does anyone have a favorite protocol for staining myelin in the brain, especially in fish >21 dpf? We would like a dye, not a line. We're looking into CMC or fluoromyelin but are unsure about penetration into the brain." Any feedback or leads would be appreciated!

🤔 #AskZebrafish

This is really helpful. We will give them your contact info in case they have any questions. Thank you!

#AskZebrafish from Slack: For folks studying metabolism, have you done chemical screens in recent years? If so, which libraries will you recommend? 🤔

#AskZebrafish: Does anyone know of a good antibody recognizing mTagBFP2? Preferably available in North America. Any help would be appreciated 🙏

Perfect! I’ll have them reach out to your email!