🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish
20.06.2025 19:14 — 👍 0 🔁 0 💬 0 📌 1
Another query from Slack: Looking for ccdc40 mutant #zebrafish (aka locke). Preferably in the US. Any leads would be much appreciated! #AskZebrafish
09.06.2025 14:22 — 👍 1 🔁 1 💬 0 📌 2
Query from Slack: Does anyone have good recommendations for antibodies that label mitochondrial DNA encoded proteins and work well in #zebrafish? #AskZebrafish
09.06.2025 14:07 — 👍 1 🔁 1 💬 0 📌 2
Anonymous query from Slack: Does anyone have the TgBAC(foxp3a:TagCFP-NTR)vcc5 line swimming around, preferably in the US? Thanks in advance! #AskZebrafish 🤔
15.05.2025 19:55 — 👍 0 🔁 0 💬 0 📌 1
Still on the hunt for fish lines 🙏
13.05.2025 17:31 — 👍 0 🔁 1 💬 0 📌 0
Still searching for leads! 🙏
01.05.2025 20:23 — 👍 0 🔁 0 💬 1 📌 0
Head-scratcher from Slack: Does anyone know if it is possible to tell (by eye/microscope) if the developing oocytes in a zebrafish ovary are at the pre-, during, or post-vitellogenesis stage? 🤔 #AskZebrafish
29.04.2025 16:19 — 👍 2 🔁 4 💬 2 📌 0
Looking for anyone w/ experience in smRNA analysis who can provide someone with information or guidance? They already have their sequences, but can't find a clear methodology/protocol to help them analyze it. The sequences were obtained from NOVOGENE's Small RNA Sequencing service. 🤔 #AskZebrafish
26.04.2025 08:58 — 👍 3 🔁 1 💬 3 📌 0
Any leads would be appreciated 🙏
22.04.2025 12:38 — 👍 2 🔁 1 💬 0 📌 0
Any leads would be appreciated! Reposts welcome 🙏
08.04.2025 17:26 — 👍 2 🔁 2 💬 0 📌 0
Still searching for leads 👀
07.04.2025 17:46 — 👍 1 🔁 2 💬 0 📌 0
Still looking for leads 🕵️
04.04.2025 14:55 — 👍 0 🔁 0 💬 0 📌 0
Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish
04.04.2025 14:42 — 👍 1 🔁 2 💬 1 📌 1
Still looking for leads 🕵️
04.04.2025 09:22 — 👍 0 🔁 1 💬 0 📌 0
Re-upping
31.03.2025 17:58 — 👍 0 🔁 0 💬 0 📌 0
Ping @bazzinilab.bsky.social Didn’t your team do something like this?
31.03.2025 17:58 — 👍 0 🔁 0 💬 1 📌 0
Did you find this yet?
31.03.2025 17:55 — 👍 0 🔁 0 💬 0 📌 0
Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish
27.03.2025 15:39 — 👍 3 🔁 2 💬 1 📌 0
🤔 #AskZebrafish
27.03.2025 13:55 — 👍 0 🔁 0 💬 0 📌 0
Thanks but we don’t think this would be classified as ‘efficiently’
21.03.2025 14:20 — 👍 0 🔁 0 💬 0 📌 0
Still looking for guide sequences! Any leads and/or help appreciated!
21.03.2025 13:08 — 👍 0 🔁 0 💬 1 📌 0
🤔 #AskZebrafish
19.03.2025 18:30 — 👍 1 🔁 2 💬 0 📌 0
Query from Slack: Does anyone have scn8aa and scn8ab #zebrafish mutants and would be willing to share some embryos? (Preferably in Europe, if possible) 🔎 #AskZebrafish
18.03.2025 12:17 — 👍 1 🔁 2 💬 0 📌 1
🤔 #AskZebrafish
17.03.2025 18:49 — 👍 1 🔁 1 💬 1 📌 0
Re-upping 🙏
12.03.2025 18:16 — 👍 0 🔁 0 💬 0 📌 0
🤔 #AskZebrafish
12.03.2025 11:17 — 👍 3 🔁 1 💬 0 📌 0
Re-upping
11.03.2025 19:50 — 👍 5 🔁 4 💬 0 📌 0
Re-upping
11.03.2025 19:50 — 👍 0 🔁 0 💬 0 📌 0
Query from Slack tankspace: “Looking for transgenic lines [preferably in Europe] marking mesoderm/mesendoderm and ectoderm, with expression before 6hpf. Thinking of mezzo and sox3, if anyone has these? Any other suggestions would also be great.” #AskZebrafish
11.03.2025 19:49 — 👍 3 🔁 5 💬 1 📌 1
#AskZebrafish from Slack: "Does anyone have a vector they recommend for poisonous inserts? We are using a bacterial strain with high expression of LacI to prevent expression from our insert and the bacteria still mutate the construct - we assume to render it a non-functional channel."
10.03.2025 15:44 — 👍 0 🔁 1 💬 0 📌 1
