Juan C. Landoni

Rare diseases are not so rare. Make a difference: www.rarediseaseday.org #RareDiseaseDay

Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction Lisci et al. present a unified model revealing how cells escape glutamine addiction, a metabolic vulnerability of several cancers. Combining systematic genetic and nutrient screens, they show that bio...

Our story on #FBXW7 and #biotin in #glutamine_addiction is now peer-reviewed and published in Molecular Cell! Heroic work led by the very first postdoc of the lab @miriamlisci.bsky.social. Have a look!

www.cell.com/molecular-ce...

Vitamins: a tool to exploit against cancer A research group at Unil has identified a new mechanism that exposes the vulnerability of tumor cells when they are deprived of vitamin B7.

🥜🦀 A research group @jourdainlab.bsky.social @dib-unil.bsky.social @fbm-unil.bsky.social @unil.bsky.social has identified a new mechanism that exposes the vulnerability of #tumor #cells when they are deprived of #vitamin B7, also known as #biotin.

Press release here 👉 www.unil.ch/news/en/1770...

Excited to share our new preprint exploring how Paramecium achieves diverse flow functions, i.e. feeding and swimming, simultaneously. This work was spearheaded by our ExM expert, PhD student Daphne Laan @daphnelaan.bsky.social :
www.biorxiv.org/content/10.6...

The "PIs of Tomorrow" competition at #LS2AM26 was such a fantastic experience, huge thanks to the org team and jury who shared their invaluable advice with us! And thanks to the audience for voting me the popular vote winner! 🎉

If you have the chance to participate of #PIOT you definitely should!

The "PIs of Tomorrow" competition was such a fantastic experience, huge thanks to the great jury who shared their invaluable advice with us, and all the @lifesciswitzerland.bsky.social AM organisers!

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence!

www.nature.com/ncomms/edito...

@natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

Organelles do NOT have a single uniform pH.
And if you think they must, because “protons diffuse fast,” this paper is for you.
A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

🎸Why this is exciting!!🦖

Quantitative super-resolution often requires genetically encoded tags to get labeling sufficient for quantitative imaging. Here with qExM we use commercial antibodies for endogenous targets.

This opens HUGE opportunities to count in tissues, organisms, or clinical samples!

We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ!

We measured the abundances for Complex I, III, and IV.

How they differ in absolute abundances in metabolically distinct subpopulations

And even how T-cell activation changes supercomplex density

How does qExM work?

We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples.

The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!

We validated qExM first on nuclear pore complexes, attempting to quantify the 8 fold symmetry without using this prior knowledge.

We achieved a mean error of just 9.4% 😍🔬🎸

With these tools in place we moved to tackle protein complex abundances that were unknown....

🔬🚨New preprint alert! 🚨🔬

We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology

www.biorxiv.org/content/10.6...

#SuperResolution #CellBiology

@lycasworks.bsky.social looks familiar? 🧐

#Mitochondria or Alien script? 🧪🔬👽

Thank you!! 🔬

My new ImageJ / Fiji toolkit is out 🔥! The goal is to make image handling & visualization easy, with an intuitive interface! Install it on Fiji with the "Image Viewer" update site
#microscopy #ImageJ #FluorescenceFriday #microscopyMonday
imagej.net/plugins/imag...

Accidentally festive #Celegans gonad while trying out some awesome LUTs by @kwolbachia.bsky.social for #FluorescenceFriday 🧪🔬

The PIs of Tomorrow session is part of the LS² Annual Meeting 2026. We have now chosen our 4 finalists:

Theodora Constantin
Juan Cruz Landoni
Olimpia Bompadre
Alaz Özcan

More info: annual-meeting.ls2.ch/2026/special...

Join our AM to see their talks: annual-meeting.ls2.ch/2026/registr...

Review title

Review sections graphic

Sub compartments of mitochondria graphic

Mitochondrial.dynamics graphic

🔬 Curious about #mitochondria #imaging but unsure where to begin? Our review might help! 🚀📜🧪

www.annualreviews.org/content/jour...

#Quantitative #Microscopy #SuperResolution #AcademicSky #ScienceSky #CellBiology #mtDNA

#StarterPack for the #Mitochondria research community! Very much a work in progress, comment below/reach out to be added!

#Mito #Mitochondriac

go.bsky.app/J6Bu5fL

Asymmetric mitochondrial trafficking maintains network morphology by balancing perinuclear biogenesis In functionally polarized cells, mitochondria can form distinct subpopulations, positioned at sites of varying metabolic and energetic demands. Thus far, the potential presence of such subpopulations ...

Co-led with @jclandoni.bsky.social and fantastic support from Keaton Holt, Elena Koslover, @tatjanakleele.bsky.social , @sulianamanley.bsky.social , Sheda Ben Nejma, and Berna Durmus, you can find the preprint here: www.biorxiv.org/content/10.6...
It's wonderful to see this project out in the wild

Using photoconversion, volumetric imaging, mitochondrial segmentation, and network simulations, we identified a distinct, proliferative mitochondrial subpopulation positioned around the nucleus. From there, single mitochondria scatter new material across the cell, maintaining network in balance

🔬🥟 What do empanadas and #mitochondria have in common?
More than you'd think. Our "empanada-shaped" cells just helped uncover hidden subpopulations of mitochondria in your everyday, unpolarized cell lines. Curious how mitochondrial trafficking shapes network health?

Fantastic collaboration led by
@julius-w.bsky.social, with the Koslover lab
@ucsdphysci.bsky.social and @tatjanakleele.bsky.social @sulianamanley.bsky.social and other non-bsky peeps.

Asymmetric mitochondrial trafficking maintains network morphology by balancing perinuclear biogenesis In functionally polarized cells, mitochondria can form distinct subpopulations, positioned at sites of varying metabolic and energetic demands. Thus far, the potential presence of such subpopulations ...

www.biorxiv.org/content/10.6...

doi.org/10.64898/2025.12.08.692722

Preprint alert 🚨 Ever wondered what role fast #mitochondria trafficking plays in the simple cells we mostly use? In @julius-w.bsky.social et al. we use mitodynamics + simulations to identify the importance of outwards transport to distribute new material and keep network balance! Link below!

Tune in! 🔬

At this week's #VirtualPub, we welcome Juan Cruz Landoni, EPFL, on "Pearling drives mitochondrial DNA nucleoid homeostasis."
🗓️ Friday, Dec 12 @ 13:00 CET
Register here🔽
www.eurobioimaging.eu/events/pearl...

A cloning strategy as depicted in the OpenCloning website

#OpenCloning is a an Open Source alternative to SnapGene/Benchling that supports automation and integration with other software

✅ Free
🔓 Open Source
🧬 More cloning methods than SnapGene
🤖 Can be automated with python
👨‍🔬 Built by a researcher — for researchers!

👉 Check it out at opencloning.org