Philippa Melamed

Deaminase-assisted single-molecule and single-cell chromatin fiber sequencing Gene regulation is mediated by the co-occupancy of numerous proteins along individual chromatin fibers. However, our tools for deeply profiling how proteins co-occupy individual fibers, especially at ...

What an amazing assay.

DAF-seq, the evolution of Fiber-seq from the Andrew Stergachis group, using the @pacbio.bsky.social platform:
www.biorxiv.org/content/10.1...

This will revolutionise our understanding of transcriptional regulation and functional non-coding variants.

Epigenetics and Gene Regulation in Health and Disease: Linking Basic Mechanisms with Therapeutic Opportunities | Keystone Symposia Join us at the Keystone Symposia on Epigenetics and Gene Regulation in Health and Disease: Linking Basic Mechanisms with Therapeutic Opportunities, March 2026, in Geneva, with field leaders!

On behalf of @ckadoch.bsky.social and myself, we are pleased to present our upcoming @KeystoneSymposia, #KSEpigenetics26 Epigenetics and Gene Regulation in Health and Disease, March 2-5, in Geneva, Switzerland. keysym.us/KSEpigenetic...

Is enhancer-driven gene regulation all wrapped up? - Nature Reviews Genetics In this Comment, Wendy Bickmore discusses mechanistic models of how 3D genome organization facilitates communication between distant enhancers and their target promoters to regulate gene expression.

www.nature.com/articles/s41...

FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics The FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics will take place 19–22 October 2026 in Budapest, Hungary. Here we give a taste of the scientific content, flag some of the speaker...

Do not forget to apply to the FEBS Advanced Lecture Course: 6th Danube Conference on Epigenetics (19–22 October 2026, Budapest, Hungary. Will be a great meeting as always. Check out the site: network.febs.org/posts/febs-a...

Pls add us also - chromatin, enhancers and transcription in the context of hormones. Thx.

FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediate Burgos-Bravo et al. use single-molecule optical tweezers to monitor the effect of the histone chaperone FACT on transcription through nucleosomes. FACT functions on nucleosomes initially unwrapped by ...

FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediate: Molecular Cell www.cell.com/molecular-ce...

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High-throughput transcriptome analysis reveals a developmental increase in Acvr1c which mediates epigenetic repression of the gene encoding the pubertal brake, Makorin ring finger protein 3 Abstract. Makorin ring finger protein 3 (MKRN3) acts as a “pubertal brake.” MKRN3 loss-of-function mutations are the most common genetic cause of central p

Latest from our lab - great work from Dor...for those interested in #Mkrn3, #puberty, #reproduction, #chromatin, #transcription, #bioinformatics.

Great to see this from a former student in my NUS lab - Adrian @drainteo.bsky.social - looks like a good read.

Optical control of gene expression using a DNA G-quadruplex targeting reversible photoswitch Nature Chemistry - Dynamic gene expression is crucial for coordinating diverse cellular activities, but challenging to modulate with high spatiotemporal precision. Now, a photoswitchable DNA...

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rdcu.be/egkqK

The R Graph Gallery – Help and inspiration for R charts The R graph gallery displays hundreds of charts made with R, always providing the reproducible code.

Ever stuck choosing the right plot or trying to figure out how to make it look just right? My go-to resource is the R Graph Gallery (r-graph-gallery.com), which is full of examples and clear code for everything from scatterplots to Sankey diagrams! #dataviz #rstats #rladies

First map of human brain mitochondria is ‘groundbreaking’ achievement Nature - Hundreds of cubes of human brain tissue help scientists to chart the energy-making capabilities of various brain regions.

🧪 Scientists have created the first map of mitochondria throughout the entire brain

https://go.nature.com/42j9YCV

CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks - Nature Communications Cleavage Under Targets & Tagmentation is a rapidly expanding technique, but thorough evaluation and benchmarking against established ChIP-seq datasets are required. This study shows that CUT&T...

We're so thrilled to finally share with you the published version of our CUT&Tag optimization and benchmarking paper "CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks" - out in @naturecomms.bsky.social today: www.nature.com/articles/s41...

our work on the molecular differences between transcription factor isoforms is out now in Molecular Cell!

key point: 2/3rds of TF isos differ in properties like DNA binding & transcriptional activity

many are "negative regulators" & misexpressed in cancer

www.sciencedirect.com/science/arti...

Two more weeks to send us your abstract! >20 abstract selected talks. Priority to junior scientists and cool biology on gene regulation (DNA/RNA/protein). Come to meet the world leaders in single molecule genomics and single molecule imaging! Help shaping the future of the field in the workshops!

G-quadruplexes and their unexpected ability to fold proteins | PNAS G-quadruplexes and their unexpected ability to fold proteins

Have a look at our newest commentary in @pnas.org :

"G-quadruplexes and their unexpected ability to fold proteins"

Full text: www.pnas.org/doi/10.1073/...

#G4 #quadruplex #proteins #folding

Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex Many genes physically interact with the nuclear pore complex (NPC), which requires transcription factors (TFs). Ge et al. show that the exportin Crm1 binds TFs and nuclear pore proteins to dock chromatin at the NPC and to stimulate transcription. This function of Crm1 is distinct from its role in nuclear export.

Exportin-1 functions as an adaptor for transcription factor-mediated docking of chromatin at the nuclear pore complex

A GnRH neuronal population in the olfactory bulb translates socially relevant odors into reproductive behavior in male mice - Nature Neuroscience Studying GnRH neuroendocrine cells in the mouse olfactory bulb (GnRHOB neurons), Decoster et al. show that these cells respond to female odors and their activation regulates males’ female-odor prefere...

Congratulations to Laurine Decoster and collaborators from our lab on getting the The SNE Impact 2024 on their paper "A GnRH neuronal population in the olfactory bulb translates socially relevant odors into reproductive behavior in male mice" 👏👏🎉🎊

www.nature.com/articles/s41...

Short-term evolutionary implications of an introgressed size-determining supergene in a vulnerable population Nature Communications - The thorny skate is a vulnerable species in the northwest Atlantic ocean with a discreet size polymorphism. Here, the authors have sequenced 49 thorny skate individuals,...

This is cool
rdcu.be/eeGyj

Women twice as likely to develop Alzheimer's disease as men - but scientists do not know why - Nature Medicine Changes to the female brain during menopause, together with social and cultural factors, might affect disease risk.

Women are twice as likely to develop Alzheimer’s disease as men. A feature in Nature Medicine reports how the changes to the female brain during menopause, along with social and cultural factors, might affect disease risk. #medsky #alzsky 🧪

New followers = time for a fresh intro! 👋

I’m Christopher, a reproductive biologist and group leader at @IBDMmarseille 🇫🇷

My lab uses #LiveImaging to explore how the dynamic #ovary produces healthy #eggs 🔬🥚

Please share & follow as we push the boundaries of #fertility research!

🔗 bit.ly/3zQPEh7

🚨 PhD Opportunity! 🚨

Passionate about ovarian biology? This cutting-edge PhD project combines live-cell microscopy & biophysical modelling to explore the dynamic ovary! 🧬🔬

I'm happy to chat more—DM or email me to set up a Zoom call 📩 And please share! 🙌🏼

christopher.thomas@univ-amu.fr

🤣🤣🤣

We have 3 openings for ambitious postdocs or PhD students in our #CDlab - for exciting single-molecule biophysics research on nuclear pores, peroxisomes, or archaeal divisomes.

Check it out and apply: ceesdekkerlab.nl/come-join-us/

RT=nice!

Fantastic study and collaboration led by stellar postdoc @ioansarr.bsky.social, it was a pleasure to host you in the lab (& thanks to @embo.org for the visiting fellowship)

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

How do replisomes walk on DNA? And what happens when they run into a G-quadruplex? @sahilbatra.bsky.social and @benallwein.bsky.social provide unexpected insight in our latest paper with Richard Hite @mskcancercenter.bsky.social @science.org www.science.org/doi/10.1126/.... Congrats to all authors!

Sensory input, sex and function shape hypothalamic cell type development - Nature Paired transcriptomic and chromatin accessibility profiling are used to examine the developmental trajectories of neuronal populations in the hypothalamic preoptic region, including cell types with ke...

Sex related development of the hypothalamus 👇

www.nature.com/articles/s41...

This was an awesome meeting last time out- #enhancer folks should register now!

Developmental timing across species | EMBO Workshop | 6-9 May - the Node Join us at the EMBO Workshop "EvoDevoTempo – Developmental Timing Across Species: From Mechanisms to Evolutionary Insights". Topics will include

"Developmental Timing Across Species"

@embo.org Workshop organised by @trayon.bsky.social @bassemh.bsky.social @mdiazcuadros.bsky.social @vanderhaeghenp2.bsky.social

📅6-9 May 2025
📍Paris

Submit abstract and register by 1 April:
thenode.biologists.com/developmenta...

#EvoDevoTempo

PRC1 and PRC2 proximal interactome in mouse embryonic stem cells Zijlmans and Stelloo et al. profiled in vivo proximal interactomes of PRC1 and PRC2 in mouse embryonic stem cells, identifying >100 proteins, including transcription factors and RNA-binding proteins. ...

PRC1 and PRC2 proximal interactome in mouse embryonic stem cells: Cell Reports www.cell.com/cell-reports...