New England Biolabs

NEB is excited to announce that the winners for the 2026 Calendar Contest have been chosen! There were so many great photos to choose from, and we thank everyone who submitted. We appreciate the support to help make next year's calendar!

Revisit our blog post demystifying Golden Gate Assembly! Learn when and how to use it and gain everything you need to add this powerful cloning method to your toolbox: https://nebiolabs.com/4pTjS83

Using or intrigued by the concept of a single/universal annealing temperature in your #PCR? Our recent application note explores this topic and how it can be used with Q5. Read more: https://nebiolabs.com/4o3h6M0

Ready to learn more about a rapid, cell-free workflow that streamlines the DNA design to protein purification process? Don’t forget to register for our webinar tomorrow at 1 pm EDT! https://nebiolabs.com/46cLLz0

In our latest blog, "Advancing DNA Barcoding at the Royal Botanic Garden Edinburgh," (https://nebiolabs.com/48BWpSD) learn how Nattanon Meeprom, Ph.D., surmounted sample challenges to accelerate data collection as part of a team evaluating Saudi Arabian ecosystems

In our latest podcast (https://nebiolabs.com/3Wbbsv8), NEB bioinformaticist Vladimir Potapov talks through the open access Ligase Fidelity Tools which are used to aid in the design of DNA Assembly with the Golden Gate Assembly method.

Webinar: Streamlining DNA assembly to protein synthesis with rapid, cell-free workflows Speaker: Matthew Norton, Ph.D., Product Marketing Manager, New England Biolabs Time: Wednesday, Oct. 1, 2025, 1:00 pm EDT

Looking for new solutions to express protein quickly and efficiently? Join us for our webinar Oct. 1 at 1 pm EDT (https://nebiolabs.com/46cLLz0), where we’ll guide you through a rapid cell-free workflow to express your proteins of interest with confidence.

There’s still time to register for our upcoming webinar tomorrow at 1 pm EDT all about getting the most out of your NEBuilder HiFi DNA Assembly reactions! https://nebiolabs.com/46cLLz0

Webinar: Get the most out of NEBuilder HiFi DNA Assembly Wednesday, Sept. 24, 2025, 1:00 pm EDT

Webinar: Streamlining DNA assembly to protein synthesis with rapid, cell-free workflows Wednesday, Oct. 1, 2025, 1:00 pm EDT

Don’t miss our upcoming webinars on Sept. 24 and Oct. 1 to learn more about NEBuilder HiFi #DNAAssembly and how to streamline your assembly to #proteinsynthesis workflow with #CFPS!
https://nebiolabs.com/46cLLz0

Webinar: Get the most out of NEBuilder HiFi DNA Assembly Speakers: Lindsey Spiegelman, Ph.D., Production Scientist I, New England Biolabs Alison Dolan, M.S., Production Scientist II, New England Biolabs Jack Martz, M.S., Technical Support Scientist I, New England Biolabs Time: Wednesday, Sept. 24, 2025, 1:00 pm EDT

Ready to get the most out of NEBuilder HiFi DNA Assembly? Register for our webinar on September 24 at 1 pm EDT, where you’ll learn more about NEBuilder HiFi’s advantages and strategies to optimize your reactions for maximum efficiency and success. https://nebiolabs.com/46cLLz0

Fall is fast approaching in the Northern hemisphere and along with it comes Viral Respiratory Illness season. Be ready with the NEBNext #RSV Primer Module: https://nebiolabs.com/3HZayOZ

#NGS

TET3 regulates hematopoietic stem cell homeostasis during embryonic and adult hematopoiesis The ten‐eleven translocation family of enzymes (TET1/2/3) promotes DNA demethylation and is essential for hematopoiesis. While the roles of TET1 and TET2 are well‐studied in hematopoiesis, the require...

Researchers at Albert Einstein College of Medicine investigated how TET3 mediates stem cell homeostasis during hemaopoiesis; An excellent showcase for NEBNext EM-seq™ and NEBNext E5hmC-seq™!

https://pmc.ncbi.nlm.nih.gov/articles/PMC12053453/

Webinar: From Templates to Discoveries: Using the NEBExpress Cell-free E. coli Protein Synthesis System for Protein Generation and High-Throughput Screening Speakers: Dr. Paula Magnelli, Ph.D. Dr. Emily Chen, Ph.D.

Missed our live webinar on using the NEBExpress Cell-Free E. coli Protein Synthesis System for quick, easy in vitro #proteinexpression? View the recording for more on #CFPS & increasing workflow efficiency through template design, experimental flexibility & automation. https://nebiolabs.com/4617BW4

Thank you to everyone who attended the West Coast NEBNext User Group Meeting! We had a great time meeting our #NGS community, listening to our amazing list of speakers and talking all things next-gen sequencing.

35,000 citations and counting! What will you discover Next?

#NEBNext products aren’t just trusted — they're published. With over 35,000 scientific peer-reviewed publications featuring NEBNext, researchers around the world are turning data into discovery. We’re proud to support your breakthroughs, every step of the way. What will you discover Next?

#NGS

Whole-genome sequencing of 490,640 UK Biobank participants - Nature A study reports whole-genome sequences for 490,640 participants from the UK Biobank and combines these data with phenotypic data to provide new insights into the relationship between human variation a...

Recent findings from the ukbiobank.bsky.social Whole-Genome Sequencing Consortium are providing the deepest analysis of the human genome to date. We’re honored that they trusted NEBNext Ultra II PCR-free reagents for the monumental task! https://www.nature.com/articles/s41586-025-09272-9

Need timely #RSV variant surveillance? Our Primer Monitor Tool continually monitors registered primer sets for overlapping #SARSCoV2 and RSV variants. https://primer-monitor.neb.com/

Regulation of a phage defence island by RptR, a novel repressor that controls restriction–modification systems in diverse bacteria Abstract. Bacteria encode a panoply of defence systems to overcome phage infection. In recent years, over 100 defence systems have been identified, with th

University of Oxford and NEB scientists identify a novel family of phage defense regulators in Nucleic Acids Research: https://academic.oup.com/nar/article/53/13/gkaf645/8198042

Old mitochondria regulate niche renewal via α-ketoglutarate metabolism in stem cells - Nature Metabolism Andersson et al. show that intestinal stem cells enriched for old mitochondria are metabolically distinct and have enhanced ability to regenerate the epithelial niche.

What can the hydroxymethylome tell us about the life and times of intestinal stem cells? Recent work from the University of Helsinki and Karolinska Institutet, featuring NEBNext E5hmC-seq™, illuminates how α-ketogluterate is a key mediator in the crypt. https://nebiolabs.com/4nijw97

Interview with Samuel Ogunsola: Celebrating African Women in STEM In this podcast, Samuel Ogunsola, co-founder of Shaping African Women in STEM, shares what inspired him to launch the program and how it has grown.

2024 Passion in Science Winner Samuel Ogunsola shares the origin of Shaping African Women in STEM (SWIS Africa), an initiative co-founded by Ogunsola to empower young African women to pursue careers in STEM through mentorship, resources and networking opportunities. https://nebiolabs.com/41AwtCR

✨ Product of the Month ✨

The NEBNext RSV Primer Module is designed for use in cDNA synthesis, amplification and library prep before RSV sequencing.

https://nebiolabs.com/3HZayOZ

Design, Assemble and Express with Confidence: Enabling High-throughput DNA Assembly and Protein Expression Workflows Cell-free protein synthesis (CFPS) can permit workflow time savings and high throughput, while avoiding several limitations associated with in vivo protein expression systems. Learn more about how you...

#Cellfree #proteinsynthesis workflows avoid several limitations associated with in vivo protein expression systems. With the right design tools, #CFPS workflows can take you from design to protein purification, save time & increase throughput. Learn more in our blog: https://nebiolabs.com/4lIf6Hd