Here, Yours Truly, sermoning about #lightsheet_courses #LISH25

@cuencam15.bsky.social synthesizes what was said today about using lightsheet in research based on her experiences in prior courses, in my lab and now at EMBL Barcelona. #LISH25 #LoPaT_alumni

@ereynaud.bsky.social is here and with him comes the #sample #prep chalktalk.

@whyvinca.bsky.social lecture on FAIR data, full of practical tips on file naming, metadata management and the importance of writing good README.txt files. #LISH25 #LoPaTs

Olaf Selchow teaches us how to deal with the #data #deluge that became synonymous with #lightsheet microscopy. #LISH25

Today, the EMBO @embo.org Practical Course on light sheet microscopy started @mpi-cbg.de. Organized by @paveltomancak.bsky.social, @ereynaud.bsky.social, Sebastian Bundschuh, and @cuencam15.bsky.social. Follow #LISH25 for updates!

Day 1 of the EMBO #LISH25
Nice to be back in Dresden!
Intense 11 days ahead of us.
@paveltomancak.bsky.social
@ereynaud.bsky.social
@events.embl.org
@mpi-cbg.de

Benedict Diederich takes us on a tour of #open_hardware solutions for light sheet microscopy, centred on his #cube design, including live demos (see toys in front of the picture). #LISH25

Followed by Sebastian Bundschuh's deep dive into #lightsheet_formation, #resolution, #phototoxicity, #configurations, and more. This is a Universe by now, and it's hard to fit it into one hour lecture, but this was the right level of introduction. #LISH25 (shorter is better 😏).

We began with an excellent lecture on the basics of microscopy in general by Riccardo Maraspini, which included demonstrations of fundamental concepts such as #diffraction, #refractive_index, #magnification, #fluorescence, and #Nyquist_sampling. #LISH25

Light sheet microscopy Light sheet microscopy is a technology that enables near-isotropic imaging of large biological specimens over time. It opens new avenues to study biological processes with unprecedented imaging speed…

We are today kicking off the two-week @embo.org practical course on #Lightsheet # Microscopy at @mpi-cbg.de in #Dresden. This is the fifth iteration of the course. Hashtag #LISH2025

meetings.embo.org/event/25-lsm

Looks like private funders, HHMI,CZI etc., are not going to “save” US research. Europe is setting up mechanisms to attract US researchers & it may work. But, common, target junior talent, don’t shower money on superstars who will be flying professors & return to US when the science winter passes.

HHMI seems to always be doing the politically correct thing. What now? Are they going to reverse all thei DEI initiatives just because the wind blows in the other direction?

Paper celebrations. Or rather papers celebration. 🎉 Inclusive. #Hydra #CephalicFurrow @mpi-cbg.de #LoPaTs

Cryptic, but promising.

Tissue bending through localized BM growth or dissolution.

Alteration in tissue morphology by intrinsic stresses in an assembling BM network.

Branching morphogenesis driven by localized deposition of interstitial matrix.

Asymmetric tissue expansion through anisotropic BM remodeling.

Maybe it's time we rethink the idea that development is a cell centric process? In this beautiful review, María-del-Carmen and @stramerlab.bsky.social discussed how the ECM underlies and influences many morphogenesis processes from wing unfolding to mammary gland development.
doi.org/10.1016/j.cd...

Start-Shape-Stop: Cell communication mechanisms controlling organ size Accurate growth control is critical for the achievement of proportional organs during animal development and repair processes. Either extra or deficie…

Hot off the press!!! Proudly presenting our lab's new review on how do cells communicate to control organ size :) We focus specially on dynamic connections that operate at different timescales to regulate organ growth and morphogenesis. #devbio #SizeandShape www.sciencedirect.com/science/arti...

The Book!

Dangerous territory, Henry. 😅

Getting ready for my train ride to the 5th EMBO course on Light Sheet MIcroscopy at @mpi-cbg.de with @paveltomancak.bsky.social, Sebastian Bundschuh, @olafselchow.bsky.social and @cuencam15.bsky.social. Got gluten free food, a book to read (biology of course), a book to show (Blaschka!)...

Silviana Amethyst from our ASB group is teaching the Computational Algebraic Geometry course at the Gene Golub #SIAM summer school in Montréal (August 4-15th: www.siam.org/programs-ini...). Topics: foundations of, software of, & advances & open problems in computational algebraic geometry 😊. Enjoy!

and I thought six years was long…

Heading to the International Congress on Invertebrate Morphology in Chile? 😄
Don’t miss the talk by our PhD student Alena Kizenko on Aug 12, on how an ancient cytoskeletal gene found a new role in tunicate development 🧬 #EvoDevo #Invertebrates
🎥👇 Developing Phallusia embryo

Latest paper elifesciences.org/articles/107... closes an important cycle in our efforts to study regeneration: week-long recordings allow us to observe the behaviour of cells during the entire course of regeneration in a crustacean leg – bright objects in movie are fluorescent nuclei of cells. 1/6

On Monday next week the amazing 5th EMBO Practical course Light Sheet Microscopy will take place so some peace and quiet before the storm! @paveltomancak.bsky.social

Join our crazy beautiful project of ExM Atlas !
One more position to fill, this time for a Research Technician at @embl.org in @gautamdey.bsky.social lab.

Come have fun with us !.

N: Kristineberg?
E: Japan
S: Cape Town
W: San Diego

Hope the hearing loss is not permanent. Otherwise, choose wisely where you sit at a table. About rest, don’t overdo it. Adrenaline down makes me almost always sick.

Hi bsky!

Do embryonic tissues have backup plans🔀? Gastruloids (model of body elongation) can build an axis through different cellular mechanisms when on adherent substrates instead of free floating. A case of developmental plasticity!

w/ A. Delahaye & ‪@bensteventon.bsky.social‬

shorturl.at/v01DO

Journalists should know that words matter. Can we please consider replacing “mainstream” with “evidence” whenever appropriate. It has a completely different connotation and in science mainstream is very often due to all evidences pointing in that direction. Especially for vaccines. Thanks!