The bumbling biochemist (Bri Bibel)

Off-target and on-target activity & effects "Adverse events" and "side effects" can be caused by off-target and/or on-target activity https://youtu.be/YSraOb15xA0 https://youtu.be/YSraOb15xA0 On-target activity/on-target effects: A mole...

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YouTube video by the bumbling biochemist Off-target and on-target activity/effects & strategies wrt CRISPR, pharmaceuticals, & PCR

Off-target and on-target activity & effects youtu.be/YSraOb15xA0

Biochemistry word parts: prefixes, suffixes, roots (with examples) to help figure out terms a non-exhaustive list of some key prefixes, suffixes, roots, etc. you may see (some lots!) https://www.youtube.com/watch?v=i3EYjveeGl4 https://youtu.be/i3EYjveeGl4 First things first – prefixes...

bit.ly/biochemwordp...

YouTube video by the bumbling biochemist Some of the most important word parts to know

I strongly believe that one of the most useful things you can do if you want to learn science is to learn your word parts! Here's a start-s! youtube.com/shorts/vBTtF...

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YouTube video by the bumbling biochemist A practical guide to scientific figures and their legends/captions

Be legendary! Scientific figures & legends, a practical guide youtu.be/E7zkEqmMzmU

bit.ly/scientificco...

YouTube video by the bumbling biochemist Scientific conferences: What to expect, how to prepare, & how to make the most of your time there!

Scientific conferences: What to expect, how to prepare, & how to make the most of your time there! (PS – hope to see some of you at #ASBMB26! youtu.be/61cjGgh-r6M

YouTube video by the bumbling biochemist Quick tip: Improve the linearity & sensitivity of a Bradford assay by taking 595(or 590)/450 ratio

Quick tip: Improve the linearity & sensitivity of a Bradford assay by taking 595(or 590)/450 ratio.
youtu.be/CUTxzcHagFE

Measuring protein concentration - the many ways... Today I finished a couple of protein purifications that I started yesterday, and I wanted to figure out what my yield was - how much pure protein did I end up with? And, if I were to look inside the l...

bit.ly/proteinmeasu...

YouTube video by the bumbling biochemist Overview of protein concentration determination methods (Bradford, BCA, UV280, etc.) – pros & cons

When it comes to protein biochemistry, you'd think measuring protein concentration would be the easy part . . . youtu.be/nSkdz_Mso7E

Elution: terms, strategies, & practical tips “Elution” is basically just a fancy word for “un-bind” or “come off.” You frequently see it in the context of chromatography as well as various solid phase extraction methods like silica-based micro s...

bit.ly/elution

YouTube video by the bumbling biochemist Gradient elution strategies for protein chromatography

Gradient elution strategies for protein chromatography
youtu.be/odwswRAM7ew

YouTube video by the bumbling biochemist Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen!

Don't be fooled by imidazole! It absorbs UV280, just like protein – on chromatogram it'll be seen! Imidazole is great for purifying His-tagged proteins, but it's a pain for when you want to detect and measure concentration of proteins using UV280 absorbance! youtu.be/JZeTMXfOtGU

YouTube video by the bumbling biochemist The bumbling biochemist’s favorite under-appreciated lab equipment

The bumbling biochemist’s favorite under-appreciated lab equipment. . . youtu.be/7ektJiflKoU

Unintentional error: Avoiding common experimental artifacts (especially batch & positional effects) Although outright fraud and research misconduct definitely does occur in science sometimes, many “reproducibility” problems in science stem from poor experimental design. And some of this comes from l...

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YouTube video by the bumbling biochemist Unintentional error: Avoiding common experimental artifacts (especially batch & positional effects)

Unintentional error: Avoiding common experimental artifacts (especially batch & positional effects): Although outright fraud and research misconduct definitely does occur in science sometimes, many “reproducibility” problems in science stem from poor experimental design. youtu.be/pas66G5gtmg

Recommended article: Hartung, T. Food for Thought Look Back in Anger – What Clinical Studies Tell Us About Preclinical Work. ALTEX 2013, 30 (3), 275–291. doi.org/10.14573/alt....

Nonfiction science book recommendations Looking for a summer reading list? I haven’t read anything other than papers and textbooks in a while, so these aren’t new books, but they are some that have stuck with me. Last updated 1/2/26 htt...

These and other book recs: bit.ly/bbbookrecs

YouTube video by the bumbling biochemist Book recs involving pharma companies acting badly (or just selfishly) &/or scientific fraud

Non-conspiracy theory books involving pharma acting badly (or just selfishly) &/or scientific fraud & how to be critical consumers: Bad pharma, Doctored, Rigor Mortis, Unreliable, Empire of Pain, No More Tears, Wonder Drug, Vaccine Race, Patenting Life, Dangerous Miracle youtu.be/e7z4_D0CrXw

Techniques for buffer exchange (Desalting columns, Dialysis, & Diafiltration): Pros, cons, & going-ons The 3 D’s of buffer exchange! There are 3 main techniques for buffer exchange of proteins (or nucleic acids): Desalting (via gel filtration/size exclusion chromatography), Dialysis, and Diafiltration ...

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YouTube video by the bumbling biochemist Techniques for buffer exchange (Desalting columns, Dialysis & Diafiltration): Pros, cons & going-ons

Techniques for buffer exchange (Desalting columns, Dialysis, & Diafiltration): Pros, cons, & going-ons youtu.be/jWBDJcPzaEU

Thanks! I will probably stick with the pre-made for now and just reuse it oodles of times :)

Thanks!

Do you have a recipe you like? I tried making my own once and just made a crappy stain (and a big mess)

I promise this isn't a paid endorsement, but InstantBlue protein stain is hands-down my favorite if anyone's looking for an SDS-PAGE stain. Bands basically appear instantaneously without background and you can save and reuse multiple times. I used it in grad school and am so glad I got some again!

YouTube video by the bumbling biochemist Reusing spin concentrators (centrifugal filters)

If you’re going to reuse a spin concentrator . . . youtu.be/flsjAarv9RA