The bumbling biochemist (Bri Bibel)

A “Quick Start Guide” for starting off in research with good habits

Some things I recommend you do/learn/etc. as early as possible in your training…

blog version: bit.ly/quickstartsc... ; downloadable: bit.ly/quickstartre...

Someone had been asking about resources for starting researchers, so I thought I'd share this quick start guide to starting research with good habits. I updated it today to make sure it had the newest links and added a couple things. Hope it can help someone!

YouTube video by the bumbling biochemist Priming pumps on an AKTA

Priming your pumps on an AKTA is important for removing air from them that can damage your system and mess up your chromatography run!
youtu.be/0br_kJTDFV4

Can't wait!

www.instagram.com/p/DPXyUaskWs...

YouTube video by the bumbling biochemist Label your lids!!!!

Label your lids! One of the key lab tips I try to drill into my trainees: label the caps of tubes and bottles and all sides of boxes (and include date and initials) youtube.com/shorts/yjwkJ...

Huge thanks to Devin Feil, Loyola Marymount University for the new headshots!

YouTube video by the bumbling biochemist Lab tip: 80% glycerol - you can pipet it easily and it's fun to make!

I love getting to teach my students fun lab tricks. Today, 80% glycerol! The greatness of glycerol without the gunk! Try preparing an 80% glycerol solution. It’s way easier to work with! Here’s a practical look at how you can do it.  
youtu.be/ATlsl42x_-A

YouTube video by the bumbling biochemist Successful size exclusion chromatography run – nice symmetrical crisp peak :)

Pretty protein purification peak :) youtube.com/shorts/0Yr0m...

YouTube video by the bumbling biochemist Enzyme fundamentals (Enzymes Part 1)

ENZYME TIME!!!!!!!!!! Part 1: Enzyme Fundamentals youtu.be/5w8_zW396MA More on enzymes: bit.ly/enzymecataly...

YouTube video by the bumbling biochemist Injecting sample into a sample loop and starting SEC run on an AKTA

It's so fun teaching my lab members protein purification! Yesterday was injecting our protein sample into a sample loop, and starting SEC run on an AKTA. I'm working to build up a series of tutorial videos for my lab members, so thought I'd share here as well. youtu.be/r5AO5ojH5D8

Today, the entire Bibel lab was finally here at the same time. And, my chemicals order arrived! It was a lot of fun! More about the Bibel lab and what we do at LMU: bibellab.lmu.build

YouTube video by the bumbling biochemist AKTA flow paths and injection valve modes – controlling the plumbing on an FPLC!

AKTA flow paths and injection valve modes – controlling the plumbing on an FPLC!

youtu.be/Wn0dRwwocOc

YouTube video by the bumbling biochemist Putting the chrom- in size exclusion chromatography (SEC) standards

Putting the chrom- in size exclusion chromatography (SEC) standards! Today we ran gel filtration standards on our size exclusion chromatography (SEC) column. And a couple of them (vitamin B12 and myoglobin) are colored! youtube.com/shorts/981nO...

Size exclusion chromatography (gel filtration) No discussion of protein purifying would be complete if you excluded Size Exclusion Chromatography (SEC). Especially since, of all the types of protein chromatography you may be usin’, size exclusion ...

more on size exclusion chromatography: bit.ly/sizeexclusio...

YouTube video by the bumbling biochemist Running size exclusion chromatography (practical look at running gel filtration on an AKTA PURE)

Running size exclusion chromatography (practical look at running gel filtration on an AKTA PURE)
youtu.be/KJ4v1FwfyP4

YouTube video by the bumbling biochemist Connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an AKTA PURE

The keys to connecting a SEC (Size Exclusion Chromatography)/Gel Filtration column to an FPLC: don't let in air; don't let the column get dry; don't let the pressure get too high. youtu.be/o0ya3EpM86o

YouTube video by the bumbling biochemist Unmodeled regions of molecular structures (those grayed out regions and dashed lines in the models)

Mind the gaps! Beware parts of the model may not be there! Scientists build structural models into experimental maps. Sometimes the experimental data is not strong enough in places, so the model has gaps! youtu.be/Vl_jcEx3jS4

Protein expression and purification Recombinant protein purification workflow Where’s the protein?! Troubleshooting and optimizing recombinant protein expression Protein chromatography Protein chromatography Protein pu...

Much more on recombinant protein expression and purification:
- Blog page of posts: bit.ly/proteinpurif...
- YouTube playlist: youtube.com/playlist?lis...

As I've begun building my lab and introducing students to protein purification, seeing their excitement, it's really hit home how incredible proteins are and how immense a privilege it is to get to explore them. I love feeling that I'm empowering my students with skills that can take them far.

YouTube video by the bumbling biochemist Desalting columns for desalting and buffer exchange

I have a feeling this desalting column is going to be one of the best uses of my start-up money! No more dialysis?! youtu.be/7Hn2_VtmFEo

Alkaline lysis (mini preps) Work in a lab & there are some “boring” experiments you have to do A LOT in order to do the “exciting” experiments that require more thought. An example many people might be overly-familiar with if th...

Approach minipreps in style, not dread :) bit.ly/minipreps

YouTube video by the bumbling biochemist Minipreps (alkaline lysis)! A practical guide to what goes on in each of the steps

Minipreps (alkaline lysis)! A practical guide to what goes on in each of the steps youtu.be/HLhazQZcZog

Alkaline lysis (mini preps) Work in a lab & there are some “boring” experiments you have to do A LOT in order to do the “exciting” experiments that require more thought. An example many people might be overly-familiar with if th...

First mini preps of the Bibel lab @LMU! And first mini preps ever for Korina! She got great yields! She also helped me record a demo video for my students in next semester’s lab so stay on the lookout for a new miniprep tutorial video! bit.ly/minipreps

YouTube video by the bumbling biochemist Lab tips for syringe-filtering largish volumes and color-coding antibiotics

Lab tips for syringe-filtering largish volumes and color-coding antibiotics
youtu.be/N3zbRbjeSN4

Why are P10s such a rarity?

YouTube video by the bumbling biochemist Resuspending, diluting, and storing primers and other "oligos" (DNA or RNA oligonucleotides): Tips

Some tips for resuspending, diluting, & working with DNA & RNA oligos youtu.be/-EXdn8Zkgj8

YouTube video by the bumbling biochemist PyMOL fundamentals

This video covers basic navigation and features, and using Uniprot and PDB in combination with PyMOL to find and show a protein with key residues in stick. youtu.be/CeTTmm5DI_k

YouTube video by the bumbling biochemist Hemoglobin part 1: Cooperative binding, allostery and 2,3-BPG (including role in altitude and HbF)

Hemoglobin part 1: Cooperative binding, allostery and 2,3-BPG (including role in altitude and HbF) youtu.be/Y4gaUbIcsvA

Thank you!