Nolan Tronche

Enterococcus faecalis redox metabolism activates the unfolded protein response to impair wound healing E. faecalis EET generates ROS, which induces the UPR in keratinocytes, inhibiting in vitro migration.

We’ve discovered how the superbug E. faecalis prevents chronic wounds from healing.

It’s not a toxin. It’s metabolism.

The bacteria use extracellular electron transport (EET) to electrochemically generate ROS, effectively "freezing" skin cells in place.

doi.org/10.1126/sciadv.aeb5297

Host infection selects for sRNA variants that drive bacterial social cheating https://www.biorxiv.org/content/10.64898/2025.12.19.695336v1

Intramacrophage RIL-seq uncovers an RNA antagonist of the Salmonella virulence-associated small RNA PinT Abstract. Salmonella virulence chiefly relies upon two major pathogenicity islands, SPI-1 and SPI-2, which enable host cell invasion and intracellular surv

RIL-seq boosts the study of sRNAs. Here comes its first application to intracellular bacteria. Plus, 10 years after its characterization, we now present an RNA sponge of Salmonella PinT.
Congrats to @kooshapour.bsky.social & great collaboration w/ @jorg-vogel-lab.bsky.social!
doi.org/10.1093/nar/...

Proud to share my first first-author article from my PhD!

A big thank to my chiefs @thicoz.bsky.social @cascaleslab.bsky.social and congratulations to all co-authors @audebertstephane.bsky.social @Caterina comas

YouTube video by Institute for Systems Biology Inside the Hidden Lives of Bacteria

It’s #PostdocAppreciation Week!

@isbscience.org Postdoc Dr. Dmitry Sutormin found gut bacteria form 3 guilds:

Stressors
Metabolizers
Growers

A survival “bet-hedging” strategy that helps them adapt.

🎥 Watch Dmitry explain: youtu.be/5LG_Ae0SiFg

SAVE THE DATE!

The 2026 UK CellularMicrobiologyNetwork meeting will be held 22nd - 23rd of June at @wwiem-qub.bsky.social in Belfast.

Researchers from the UK and beyond are invited to share exciting work on the mechanisms of host-pathogen interactions.

Follow us to stay tuned! www.ukcellmicro.org

A guide to single-particle tracking - Nature Reviews Methods Primers Single-particle tracking is a method for analysing different motion behaviours of individual proteins and protein complexes in live cells. This Primer outlines the experimental setup and labelling tec...

Really enjoying reading this primer on single particle tracking #microscopy by @svanteeffelen.bsky.social and colleagues www.nature.com/articles/s43... (pdf on Sven's website)

👏👏 @lcbofficiel.bsky.social

A Small RNA Derived From the 5′ End of the IS200 tnpA Transcript Regulates Multiple Virulence Regulons in Salmonella typhimurium We show deletion of an IS200-encoded sRNA (5′tnpA) in Salmonella impacts the expression of SPI-1, flagellar, cysteine, and phsABC regulons. Evidence is presented that, with regard to the SPI-1 regulo...

Trussler et al. use comparative RNA-seq to explore how an sRNA derived from a transposon affects >200 genes and acts as global regulator of Salmonella invasion and flagellar genes.
onlinelibrary.wiley.com/doi/10.1111/...
#MicroSky #sRNA

Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome Abstract. Recent evidence indicates that the bacterial Rho helicase regulates Bacillus subtilis gene expression in a growth-dependent manner. This regulati

New cool work from French friends and colleagues: identification of Rho termination sites in B. subtilis! academic.oup.com/nar/article/...

#SubtiWiki @narjournal.bsky.social @inrae-france.bsky.social

🚨🔥 Happy to share our latest Preprint! 🔥🚨

We describe some very simple experimental approaches employed in our group to assess CRISPRi effectiveness in Mycobacteria 🧬 🦠 🧫

Project co-led by Vanessa, Wafaa, Janïs & Eliana - Our Fantastic 4 Female Scientists💫🙌🧨

www.biorxiv.org/cgi/content/...

Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility Bacteria have evolved multiple strategies to thrive in diverse environments. These include the ability to make rapid transitions between motile and se…

Swimming or sessile: the interplay between c-di-GMP signalling and flagellar motility

Current Opinion in Microbiology from Victor Sourjik

www.sciencedirect.com/science/arti...

RtmR is a membrane-embedded RRM-family RNA-binding protein that regulates biofilm formation https://www.biorxiv.org/content/10.1101/2025.07.10.664275v1

Functional interplay between (p)ppGpp and RNAP in Acinetobacter baumannii https://www.biorxiv.org/content/10.1101/2025.07.04.663200v1

🎉 I'm thrilled to share that I’ll be joining the University of Arizona as an Assistant Professor starting January 2026!

I’ll be recruiting PhD students, lab technicians, and postdocs—so if you're interested in host-microbe interactions, I’d love to hear from you!

Flagellar switch inverted repeats impact heterogeneity in flagellar gene expression and thus C. difficile RT027/MLST1 virulence Clostridioides difficile (C. difficile) RT027 strains cause infections that vary in severity from asymptomatic to lethal, but the molecular basis for …

Flagellar switch inverted repeats impact heterogeneity in flagellar gene expression and thus C. difficile RT027/MLST1 virulence www.sciencedirect.com/science/arti...

RNA-RNA Interactome Approaches Provide in vivo Evidence for a Critical Role of the Hfq Rim Face in sRNA-mRNA Pairing https://www.biorxiv.org/content/10.1101/2025.06.26.660752v1

A novel mechanism for bacterial sporulation based on programmed peptidoglycan degradation https://www.biorxiv.org/content/10.1101/2025.06.26.661752v1

Ingénieur de recherche en microbiologie, Laurent Loiseau explore les rouages du monde microbien depuis plus de trente ans. Il est récompensé par la médaille de cristal du @cnrs.fr

👉 www.insb.cnrs.fr/fr/cnrsinfo/...

How a Biofilm’s Strange Shape Emerges From Cellular Geometry | Quanta Magazine Micro decisions can have macro consequences. A soft matter physicist reveals how interactions within simple cellular collectives can lead to emergent physical traits.

Reading:

How a Biofilm’s Strange Shape Emerges From Cellular Geometry www.quantamagazine.org/how-a-biofil... via @QuantaMagazine

𝔟α𝐜𝐓𝕖Ř𝒾ⒶĹ ＳＨⓐ𝓹Ẹ 丂ħ𝓲ƒ𝓉ⒺⓇร 🦠

Ultra-sensitive RNA-seq and FISH show 𝘉𝘢𝘤𝘵𝘦𝘳𝘰𝘪𝘥𝘦𝘴 𝘵𝘩𝘦𝘵𝘢𝘪𝘰𝘵𝘢𝘰𝘮𝘪𝘤𝘳𝘰𝘯 cells adopt different shapes reflecting distinct gene expression profiles and metabolic specializations

A deliberate survival strategy for the ever-changing gut environment?

www.cell.com/cell-reports...

#microsky #rnasky

Postdoctoral Fellow position–Investigating the Escherichia coli efflux network and cell envelope to advance next-generation antimicrobial development in the Cox lab, Department of Molecular and Cellular Biology, UoG, Canada. Please share! www.coxlab.ca/job-opportun... @uofguelph.bsky.social

Detection of stress-dependent m5C rRNA dynamics in E. coli using m5C-Rol-LAMP: qPCR-based method to quantify site-specific m5C in total bacterial RNA =>stress (heat, stressox) dependent changes in rRNA, from @kirstenjunglab.bsky.social lab 🦠 #rnasky
www.biorxiv.org/content/10.1...

A shared alarmone–GTP switch controls persister formation in bacteria - Nature Microbiology Alarmone synthesis depletes GTP levels leading to a GTP-dependent switch that controls triggered, spontaneous and antibiotic-induced persister formation in Bacillus subtilis.

Read the corresponding full paper here:

www.nature.com/articles/s41...

Lon-dependent proteolysis in oxidative stress responses #microsky review from the @chienlab.bsky.social 🦠 journals.asm.org/doi/10.1128/...

There have been a lot of new additions to the starter pack. If you are into any aspects of microbial cell biology or cell biology in general, now’s a great time to follow and engage with the new arrivals 🦋
go.bsky.app/L3L2iFz

Marie Curie fellowship, post-doc hosting oppotunity in my lab. MSCA Post-doctoral Fellowship at IBPC Paris on RNA modifications in bacteria (FRANCE) 🦠💫 euraxess.ec.europa.eu/jobs/hosting...

École Thématique de Microbiologie Moléculaire 2025 - LCB L’ETMM vise à diffuser les nouveaux concepts et méthodologies en microbiologie en présentant des recherches de pointe inédites. Dans ce but, l’ETMM réunit des

​📢 Dernière places disponibles !!!

Du 6 au 10 octobre 2025 à Carry-le-Rouet, rejoignez-nous pour une semaine dédiée aux avancées en microbiologie moléculaire.

lcb.cnrs.fr/etmm2025/

#MicrobiologieMoléculaire #ETMM2025 #FormationScientifique #CNRS #Recherche #Science

Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms c-di-GMP and HipH function as a toxin-antitoxin-like module to regulate persister cell formation in bacterial biofilms.

Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms
-in #eLife
elifesciences.org/articles/99194