Bio-protocol

Thermus thermophilus CRISPR Cas6 Heterologous Expression and Purification

Work by Junwei Wei, Mohamed Motawaa and Yingjun Li at Huazhong Agricultural University.

#LifeSciences #Reproducibility #Microbiology

To learn more about the work from our latest issue, click on the full protocol link 👉https://bio-protocol.org/en/bpdetail?id=5383&type=0&source=Bluesky

#Reproducibility #LifeSciences

On the cover ✨:
Merged GFP (green) and mCherry (red) fluorescence in rice protoplasts co-expressing both proteins from a single plasmid. The image, enhanced with AI due to microscope limits, showcases cells transformed using an optimized transient system.

Using Beads as a Focus Fiduciary to Aid Software-Based Autofocus Accuracy in Microscopy

Work by Isabel Gibson, Elizabeth Julie Osterlund and Ray Truant at McMaster University.

#LifeSciences #Reproducibility #CellBiology

Work by Jade Jansen, Teunis B.H. Geijtenbeek and Neeltje A. Kootstra at University of Amsterdam.

#LifeSciences #Reproducibility #Microbiology

Inducible HIV-1 Reservoir Reduction Assay (HIVRRA), a Fast and Sensitive Assay to Test Cytotoxicity and Potency of Cure Strategies to Reduce the Replication-Competent HIV-1 Reservoir in Ex Vivo PBMCs:
bio-protocol.org/en/bpdetail?...

Our Special Issue on RNA Detection Technologies is accepting presubmission inquiries. 🔗 bio-protocol.org/en/psi?source=
Highlight your work in RNAseq, FISH, CRISPR-tagging, click chemistry, and more!

#RNA #OpenScience #LifeSciences #Technology

Bio-protocol is freely accessible to readers but there are additional benefits of becoming a registered user. Register at bio-protocol.org/nlogin.aspx!

Isolation and Culture of Ferret Airway Stem Cells

Work by Ziying Yan, John F. Engelhardt and Feng Yuan at University of Alabama at Birmingham.

#LifeSciences #Reproducibility #StemCell

A Novel Composite Method of Post-stroke Epilepsy Induction

Work by Yiting Guo and Raymond Tak Fai Cheung at The University of Hong Kong.

#LifeSciences #Reproducibility #Neuroscience

Flow Cytometric Quantification of HIV-1-Infected Cells Expressing Either Abortive or Elongated HIV-1 Transcripts Using Flow-FISH

Work by Shirley Man, Teunis B.H. Geijtenbeek and Neeltje A. Kootstra at Amsterdam UMC Location University of Amsterdam.

#LifeSciences #Reproducibility #Microbiology

Workflow for Fluorescence-Targeted Lamella Milling From Vitrified Cells With a Coincident Fluorescence, Electron, and Ion Beam Microscope

Work by Elise G. Perton, Daan B. Boltje, Arjen J. Jakobi and Jacob P. Hoogenboom at Delft University of Technology.

#LifeSciences #Reproducibility #Biophysics

Quantifying Intracellular Distributions of HaloTag-Labeled Proteins With SDS-PAGE and Epifluorescence Microscopy

Work by Julia Shangguan and Ronald S. Rock at University of Chicago.

#LifeSciences #Reproducibility #CellBiology

✨Special Issue Editors:
@kifla.bsky.social‬ | @marionhogg.bsky.social | Renate Weizbauer PhD

#LifeSciences #RNA #OpenScience #Technologies

From single-cell RNA-seq to in situ imaging & click chemistry — this issue will spotlight innovations advancing how we detect and study RNA.
✨ Interested in contributing?
Submit a pre-submission inquiry here!

📢 Call for Submissions!
We’re excited to launch a Special Issue on Technologies for RNA Detection at Bio-protocol!

#LifeSciences #RNA #OpenScience #Technologies

Check out the latest step-by-step protocols in various #LifeSciences fields.

#Biochemistry #BioinformaticsandComputationalBiology #BiologicalEngineering #Biophysics #CellBiology #Microbiology #MolecularBiology #Neuroscience #PlantScience #StemCell

Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...

Quantification of Neural Progenitor Cells From Zika Virus-Infected Zebrafish Embryos

Work by Yago C. P. Gomes, Aïssatou Aïcha Sow, Shunmoogum A. Patten and Laurent Chatel-Chaix at Institut National de la Recherche Scientifique.

#LifeSciences #Reproducibility #Neuroscience

To explore more of our webinar series, New Tools of the Trade in Life Sciences: Technologies that Enable Breakthroughs, please visit: bio-protocol.org/webinar
Follow Bio-protocol to stay informed on the latest methods and protocols in #lifesciences.

YouTube video by Bio-protocol Webinars Synapse by synapse: High-speed cortical connectomics using optomapping | Webinar | Neuroscience

Now watch the recording of our webinar, "Synapse by synapse: High-speed cortical connectomics using optomapping", on our YouTube channel! ​

🔗 👉 youtu.be/8FvBlf1iR1o?...

#Webinar #LifeSciences

Protocol for Generation of Single-Gene Knockout in Hard-to-Transfect THP1 Cell Lines Using CRISPR/Cas9

Work by Kaveri Srivastava and Bhaswati Pandit at BRIC-National Institute of Biomedical Genomics.

#LifeSciences #Reproducibility #MolecularBiology

Love RNA biology and care about reproducibility? I’m co-editing a @bio-protocol.bsky.social special issue with @marionhogg.bsky.social & Renate Weizbauer —focusing open access and transparent RNA detection protocols.
Submit yours today - en.bio-protocol.org/content17
#OpenScience #ReproRNAbiology

Submit a protocol to the Bio-protocol journal at bio-protocol.org/en/psi.
In line with our mission to improve research reproducibility, we welcome submissions of both newly developed and classic protocols that lack detailed descriptions elsewhere in the literature.

To learn more about the work from our latest issue, click on the full protocol link 👉
bio-protocol.org/en/bpdetail?...

#Reproducibility #LifeSciences

On the cover ✨:
The image displays six kidney glomeruli with fluorescent labeling of podocytes, imaged by the authors. These visuals highlight the target cells isolated using the protocol featured in this article.

APEX2 RNA Proximity Labeling in Mammalian Cell Lines With Low Biotin Permeability

Work by Adrian Beat Tschan, Arpan Kumar Rai, Lucas Pelkmans, Alexa B.R. McIntyre at University of Zurich, Zurich.

#LifeSciences #Reproducibility #Molecular Biology

Evaluation of In Vitro Cytotoxic Activity of CAR-T Cells Using Patient-Derived Organoids

Work by Lei Yu at Shenzhen Institutes of Advanced Technology.

#LifeSciences #Reproducibility #Immunology

Derivation and Culture of Enriched Phrenic-Like Motor Neurons From Human iPSCs

Work by Louise Thiry, Julien Sirois, Thomas M. Durcan and Stefano Stifani at McGill University.

#LifeSciences #Reproducibility #Neuroscience

An Alternative Gene Editing Strategy Using a Single AAV Vector

Work by Jamie C. Moffa, Vani Kalyanaraman and Bryan A. Copits at Washington University School of Medicine in St. Louis.

#LifeSciences #Reproducibility #Neuroscience