Assessing Temperature-Dependent DNA Cleavage by CRISPR-Cas9
Work by Alexa L. Knight, Jinping Luo and George P. Lisi at Brown University.
#LifeSciences #Reproducibility #MolecularBiology
14.10.2025 02:38 — 👍 0 🔁 0 💬 0 📌 0
Artificial Metalloenzymes in Artificial Sanctuaries Through Liquid–Liquid Phase Separation
Work by Kaixin Wang, Guangjie Zhang, Lei Zhang, Yugang Bai and Tong Wu at Hunan University.
#LifeSciences #Reproducibility #BiologicalEngineering
13.10.2025 02:41 — 👍 1 🔁 0 💬 1 📌 0
Rapid and Uniform NHS-Ester-Based Membrane Protein Labeling of Live Mammalian Cells
Work by Alyssa Burgess, Hirushi Gunasekara and Ying S. Hu at University of Illinois Chicago.
#LifeSciences #Reproducibility #CellBiology
11.10.2025 01:54 — 👍 0 🔁 0 💬 0 📌 0
A Protocol Guide to Micro Milling for Bio-Microfluidics
Work by Hannah L. Viola, Vishwa Vasani and Shuichi Takayama at Georgia Institute of Technology.
#LifeSciences #Reproducibility #BiologicalEngineering
09.10.2025 13:50 — 👍 2 🔁 0 💬 0 📌 0
Bio-protocol's next #webinar on, Optimizing live-cell imaging: From probe to FLIM-STED integration, is just a week away!
Have you registered yet? 🔗s.bio-protocol.org/4e8ab88f7f28...
🗓 Date: October 15, 2025
⏰ Time: 11:00AM EST
Speaker: @magaliebenard.bsky.social ,
Moderator: Christophe Chamot
08.10.2025 23:05 — 👍 1 🔁 1 💬 0 📌 0
Check out the latest step-by-step protocols in various #LifeSciences fields.
#BioinformaticsandComputationalBiology #BiologicalEngineering #CancerBiology #CellBiology #MolecularBiology #PlantScience #StemCell
06.10.2025 11:07 — 👍 0 🔁 0 💬 0 📌 0
Bio-protocol's new issue is now live! 🎉
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06.10.2025 11:07 — 👍 0 🔁 0 💬 1 📌 0
YouTube video by Bio-protocol Webinars
Proteome Birthdating: A single-sample approach to measuring protein turnover and age | Webinar
The recording of our recent webinar, "Proteome Birthdating: A single-sample approach to measuring protein turnover and age" by Michael Meadow, PhD and Emilie Besnard, PhD, is now available!
Watch the recording on our YouTube channel!
🔗 👉 youtu.be/NGdjN9xlkWY
#Webinar #LifeSciences
05.10.2025 11:51 — 👍 2 🔁 0 💬 0 📌 0
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02.10.2025 14:22 — 👍 0 🔁 0 💬 0 📌 0
📢 Reminder: Don't miss our upcoming webinar, "Optimizing live-cell imaging: From probe to FLIM-STED integration"!
🗓 Date: October 15, 2025
⏰ Time: 11:00 AM EST
Speaker: Magalie Benard (@magaliebenard.bsky.social)
Moderator: Christophe Chamot
🔗 Register now!
#Webinar #LifeSciences
02.10.2025 14:21 — 👍 1 🔁 0 💬 1 📌 0
Preparation and Characterization of Lipid Nanoparticles Co-loaded With DNA and Nitro-Oleic Acid
Work by Manthan N. Patel, Sachchidanand Tiwari and Jacob S. Brenner at University of Pennsylvania.
#LifeSciences #Reproducibility #BiologicalEngineering
01.10.2025 17:35 — 👍 0 🔁 0 💬 0 📌 0
Work by Yunqi Li and Min Wu at Chengdu Medical College, and Ruoyang Zhao at University of Chinese Academy of Sciences.
#LifeSciences #Reproducibility #MolecularBiology
28.09.2025 05:21 — 👍 1 🔁 0 💬 0 📌 0
Cost-Effective and Reproducible Preparation of mRNA-Loaded Lipid Nanoparticles Using a Conventional Laboratory-Scale Microfluidic Assembly System:https://bio-protocol.org/en/bpdetail?id=5458&type=0&source=Bluesky
28.09.2025 05:21 — 👍 0 🔁 0 💬 1 📌 0
Isolation and Co-culture of Paneth Cells and Intestinal Stem Cells
Work by Ryosuke Isotani, Masaki Igarashi, Masaomi Miura and Toshimasa Yamauchi at The University of Tokyo.
#LifeSciences #Reproducibility #StemCell
26.09.2025 15:51 — 👍 0 🔁 0 💬 0 📌 0
Practical Guide to In Vitro Clonal Propagation of Nicotiana benthamiana Using Axillary Shoot Induction
Work by Pradeep Chand Deo at Plant Cell & Tissue Culture Professional.
#LifeSciences #Reproducibility #PlantScience
25.09.2025 03:43 — 👍 0 🔁 0 💬 0 📌 0
Work by Phuong Nguyen and Erliang Zeng at University of Iowa.
#LifeSciences #Reproducibility #BioinformaticsandComputationalBiology
24.09.2025 05:31 — 👍 0 🔁 0 💬 0 📌 0
A Protocol for Weighted Gene Co-expression Network Analysis With Module Preservation and Functional Enrichment Analysis for Tumor and Normal Transcriptomic Data:
bio-protocol.org/en/bpdetail?...
24.09.2025 05:31 — 👍 0 🔁 0 💬 1 📌 0
📢Bio-protocol Webinars present: Optimizing live-cell imaging: From probe to FLIM-STED integration
🔗Register now!
Date: October 15, 2025
⏰ Time: 11:00 AM EST
Speaker: Magalie Bénard, Ph.D.
Moderator: Christophe Chamot
This webinar is supported by Leica Microsystems. @leicamicrosystems.bsky.social
23.09.2025 22:40 — 👍 3 🔁 1 💬 0 📌 1
Check out the latest step-by-step protocols in various #LifeSciences fields.
#Biochemistry #BioinformaticsandComputationalBiology #BiologicalEngineering #CancerBiology #CellBiology #DevelopmentalBiology #Microbiology #MolecularBiology #Neuroscience #PlantScience #StemCell
23.09.2025 05:31 — 👍 0 🔁 0 💬 0 📌 0
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/en/archive?v...
23.09.2025 05:31 — 👍 3 🔁 0 💬 1 📌 0
NanoPDLIM2-Based Combination Therapy for Lung Cancer Treatment in Mouse Preclinical Studies
Work by Thi Hoa Le et al. at University of Southern California Keck School of Medicine, and Fan Sun at University of Pittsburgh School of Medicine.
#LifeSciences #Reproducibility #CancerBiology
22.09.2025 04:31 — 👍 1 🔁 0 💬 0 📌 0
Training Mice to Perform Attentional Set-Shifting Under Head Restraint
Work by John J. Stout et al. at University of Connecticut School of Medicine, and Katarina Kalajzic at University of Connecticut.
#LifeSciences #Reproducibility #Neuroscience
19.09.2025 05:14 — 👍 1 🔁 0 💬 0 📌 0
Quantitative Microscopy for Cell–Surface and Cell–Cell Interactions in Immunology
Work by Beatriz Díaz-Bello, Dalia El Arawi, Rémy Torro, Patrick Chames, Kheya Sengupta and Laurent Limozin at Turing Center for Living Systems.
#LifeSciences #Reproducibility #Immunology
18.09.2025 10:05 — 👍 0 🔁 0 💬 0 📌 0
Novel Experimental Approach to Investigate Immune Control of Vascular Function
Work by Taylor C. Kress, Candee T. Barris, Simone Kennard and Eric J. Belin de Chantemèle at Medical College of Georgia at Augusta University.
#LifeSciences #Reproducibility #Immunology
17.09.2025 03:34 — 👍 0 🔁 0 💬 0 📌 0
Simultaneous RNA Fluorescent In Situ Hybridization and Immunofluorescent Staining of Mouse Muscle Stem Cells on Fresh Frozen Skeletal Muscle Sections
Work by Vedant R. Lakkundi et al. at University of Southern California.
#LifeSciences #Reproducibility #StemCell
16.09.2025 10:12 — 👍 0 🔁 0 💬 0 📌 0
New Approach to Detect and Isolate Rhamnogalacturonan-II in Arabidopsis thaliana Seed Mucilage
Work by Dayan Sanhueza and Susana Saez-Aguayo at Universidad Andrés Bello.
#LifeSciences #Reproducibility #PlantScience
15.09.2025 05:46 — 👍 0 🔁 0 💬 0 📌 0
Ultrafast Isolation of Synaptic Terminals From Rat Brain for Cryo-Electron Tomography Analysis
Work by Rong Sun and Qiangjun Zhou at Vanderbilt University.
#LifeSciences #Reproducibility #Neuroscience
12.09.2025 07:01 — 👍 1 🔁 0 💬 0 📌 0
Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbow
Work by Thomas J. Versosky, Dilshodbek U. Nishonov and Li-Chun Tu at Ohio State University.
#LifeSciences #Reproducibility #Biophysics
11.09.2025 03:15 — 👍 1 🔁 0 💬 0 📌 0
Use of a High-Affinity Ubiquitin-Binding Domain to Detect and Purify Ubiquitinated Substrates and Their Interacting Proteins
Work by Nitu Saha, Mengwen Zhang and Mark Hochstrasser at Yale University.
#LifeSciences #Reproducibility #Biochemistry
10.09.2025 06:49 — 👍 1 🔁 0 💬 0 📌 0
Bio-protocol's new issue is now live! 🎉
bio-protocol.org/cn/archive?v...
Check out the latest step-by-step protocols in various #LifeSciences fields.
#Biochemistry #Biophysics #CancerBiology #Immunology #MolecularBiology #Neuroscience #PlantScience
09.09.2025 05:00 — 👍 1 🔁 0 💬 0 📌 0
