Carter Lab

Our latest work, in collaboration with the terrific @kashishsingh.bsky.social in the Carter lab @mrclmb.bsky.social is out. So proud of this study and everyone involved...

Cryo-EM structures of Egl-BicD-RNA complexes reveal how diverse mRNAs are selected for subcellular localization Localization of mRNAs is a widespread mechanism for controlling where proteins operate in cells and underpins many fundamental processes, from embryonic patterning to synaptic plasticity. This spatial...

Here is the correct link: www.biorxiv.org/content/10.1...

Excited to share our latest work with @simonbullock11.bsky.social! We looked at how diverse mRNAs get selected for subcellular localization and it turns out that a single protein can recognize different RNA elements using shared features that weren’t apparent before.
www.biorxiv.org/content/10.1...

Check out this exciting new study from the @rgassmann.bsky.social lab that we are thrilled to be part of — revealing that CDR2 is a dynein adaptor that regulates ER sheet organization!!

TinA enables kinesin-14/KlpA to exhibit processive minus-end-directed motility Kinesin-14 motors contribute to spindle assembly by localizing to spindle poles and anchoring the minus ends of spindle microtubules. Unlike other kinesin-14 motors, KlpA uniquely exhibits plus-end-di...

In Today's #journalclub, we had a look at how TinA can make the kinesin-14 KlpA to switch to minus-end processivity on microtubules in vitro:

www.biorxiv.org/content/10.1...

Today in #journalclub, we looked at this fantastic new method from the @doudna-lab.bsky.social in Nature Biotechnology! They show how CRISPR–Csm enables single-molecule live-cell imaging of endogenous RNAs. Super cool!
www.nature.com/articles/s41...

A new study reveals how alternative splicing fine-tunes dynein cargo specificity! ZYG-12/Hook uses different isoforms to recruit dynein to either early endosomes or the nuclear envelope, showcasing a clever mechanism for organelle-specific transport. #journalclub
www.molbiolcell.org/doi/abs/10.1...

Cryo-EM captures early intermediate steps in dynein activation by LIS1 Cytoplasmic dynein-1 (dynein) is an essential molecular motor in eukaryotic cells. Dynein primarily exists in an autoinhibited “Phi” state and requires conformational changes to assemble with its cofa...

Today in #journalclub, we looked at the new study from Nguyen et al. showing how Cryo-EM captures early steps in dynein activation by LIS1. They identified a novel ‘Pre-Chi’ intermediate, shedding light on how LIS1 relieves dynein autoinhibition!
www.biorxiv.org/content/10.1...

Kinetochore dynein is sufficient to biorient chromosomes and remodel the outer kinetochore - Nature Communications Mitotic chromosomes recruit multiple microtubule-directed activities to ensure their faithful segregation. Using an approach where only one major activity is present, the authors reveal sufficiency of...

First #JournalClub on the blue side: an exciting story about dynein at the kinetochore and its role in biorientation!

www.nature.com/articles/s41...

As my lab's first post on Bluesky. If you are a UK Structural Biologist please come to our one day meeting on Friday 6th December
Lots of talk slots to be selected from abstracts.
Deadline for abstracts is TOMORROW: Friday 15th Nov.
bit.ly/4fDEqfZ

❗️Exciting science alert!! Come join us at the BCA Winter Meeting at the MRC Laboratory of Molecular Biology, Cambridge, 6th December. Short talks available - a great opportunity for early career researchers to present.. bit.ly/4fDEqfZ

Thanks!

Could you add me too? Would like to start our regular Journal Club posts here on Friday.

Our new preprint from Briggs lab is out! We solved the in-virus #cryoEM structure of the HIV matrix protein by SPA. The structure led us to a discovery resolving a long mystery in HIV biology – a peptide SP2 triggers the maturation of the HIV matrix domain. A 🧵 1/x
Paper: tinyurl.com/c7szhtfa

Nice to see @pilhoferlab.bsky.social here !!