Aashrith Saraswathibhatla

Saraswathibhatla lab is open for business!!

Have you ever thought about inflating tissues?
Or maybe quickly deflating those inflated tissues?

New #EpithelialMechanics pre-print: doi.org/10.1101/2025...
🧵 with pressure control, multiscale buckling, controlled wrinkling

Thank you for highlighting our preprint, Sharvari!! @prelights.bsky.social

Hypertrophic adipocytes increase extracellular vesicle-mediated lipid release and reprogram breast cancer cell metabolism Obesity worsens cancer-specific survival and all-cause mortality for women diagnosed with breast cancer. Rich in adipose tissue, the breast exhibits increased adipocyte size in obesity, which correlat...

Preprint alert! Excited to share this manuscript from my PhD which aims to answer an age-old question. Does size matter? For fat cells, or adipocytes, the answer seems to be yes. We found that larger adipocytes promote aggressive behavior in breast cancer cells. 1/5 www.biorxiv.org/content/10.1...

Stretch-induced endogenous electric fields drive directed collective cell migration in vivo - Nature Materials Electric fields guide collective cell migration in developing embryos of Xenopus laevis via a voltage-sensitive phosphatase.

REPOSTING this here just to officially leave "the other place"

Our work on the role of endogenous electric fields in guiding collective cell migration during #morphogenesis is out @naturematerials.bsky.social
nature.com/articles/s41...

Thank you!

Non-linear rheology of melted cheddar cheese The rheology of melted cheese is a fundamental parameter in the preparation of cheese for consumer foods, but remains poorly understood. We show that …

Understanding the physics of our foods can inform the design of future foods - plant-based or otherwise! Check out the article here: www.sciencedirect.com/science/arti...

Thank you, Jake!

Someone awesome in my circle shared some terrific news with me today but was hesitant to do so because of all that is going on in the science world.

It may appear insensitive that you are celebrating when other colleagues are struggling but I entreat you to celebrate & share good news. 1/

Thank you, Jacky! Nice meeting you recently at the GRC.

Thank you!!

Swirling motion of breast cancer cells radially aligns collagen fibers to enable collective invasion In breast cancer (BC), radial alignment of collagen fibers at the tumor-matrix interface facilitates collective invasion of cancer cells into the surrounding stromal matrix, a critical step toward met...

I want to thank all the co-authors (particularly Md Foysal Rabbi from Taeyoon Kim's lab), collaborators, and funding sources for all the help! Here is the link to the paper (11/11): biorxiv.org/content/10.1....

Finally, using an organotypic model mimicking DCIS, we demonstrate that the basement membrane acts as a mechanical insulator, preventing swirling cells from aligning collagen. Thus, after breaching the BM, swirling of BC cells radially aligns collagen (10/11)

Using traction deformation microscopy and fiber model simulations, we confirmed our hypothesis of shear-induced radial contractile stresses in collagen-rich ECMs (9/11)

Based on this, we hypothesized that shear stresses from the swirling motion of cancer cells at the tumor-matrix interface result in radially contractile stresses due to negative normal stress, and such radial stresses, in turn, align col1 radially to facilitate invasion (8/11)

Interestingly, fibrous networks exhibit the property of negative normal stress, where shear forces generate significant contractile forces perpendicular to the shear direction. This was described by Paul Janmey in 2006 and is similar to shear-normal coupling (7/11)

To our surprise, cells migrated tangentially to the tumor-matrix interface in a swirling-like motion. This raised the question: How does the swirling, or tangential, motion of cancer cells at tumor-stroma interface align collagen radially? (6/11)

Prior to the collective invasion, cells radially aligned collagen at the tumor-matrix interface in a TACS-3-like manner, which enabled their collective invasion (5/11)

Consistent with our reasoning, increasing mechanical plasticity of collagen-rich ECMs facilitated invasion, with increasing stiffness potentiating a transition from single cell to collective invasion (4/11)

We reasoned that mechanical plasticity of collagen-rich ECMs will enable cells to remodel collagen and enable their collective invasion. For this, we characterized the mechanics of human tissues and developed collagen-rich biomaterials that mimicked them (3/11)

The radial alignment of collagen fibers (TACS-3) at the tumor-matrix interface is a pathological signature of breast cancer. This was first described by Prof. Paolo Provenzano in late Keely's lab in 2006. However, it is unclear how cancer cells can radially align collagen fibers (2/11)

Despite the current uncertainty of being a scientist, I am excited to share our manuscript demonstrating an interesting mechanism of how swirling motion of breast cancer cells align collagen fibers radially at the tumor-stroma interface to enable their invasion (1/11) @thechaudhurilab.bsky.social

Our latest paper providing a biophysical mechanism of collagen fiber alignment that enables collective invasion in breast cancer!