Kermit Murray

Characterization of Amidine‐Based Degradation Products of Novichok A‐Series Nerve Agents Using Single‐Quadrupole GC–MS ABSTRACT Rationale The identification of Novichok A-series nerve agents depends primarily on the detection of stable degradation products, as intact agents are rarely encountered in environmental or forensic samples. However, limited GC–MS reference data exist for amidine-based degradation products of A-series nerve agents relevant to Chemical Weapons Convention (CWC) verification. This study aims to address a critical analytical gap supporting forensic attribution and international chemical weapons verification. Methods A series of eight N,N-dialkylethanimidamide compounds relevant to predicted Novichok degradation pathways was synthesized under controlled conditions. Samples were analyzed by GC–MS using electron ionization (EI) and positive chemical ionization (PCI) with isobutane reagent gas. Spectral data, comprising major fragment ions, protonated molecules, and adduct ions, were acquired using a GC–MS system under optimized analytical conditions. GCRI were determined using the Van den Dool and Kratz method with n-alkane standards. Results EI mass spectra showed reproducible fragmentation dominated by α-cleavage, producing characteristic low-mass ions, particularly m/z 42 [C2H4N]+ and m/z 71 [C3H7N2]+, with isomer dependent base peaks and relative abundances. As a complementary technique, PCI with isobutane yielded spectra with molecular mass confirmation via [M+H]+ ions, with minimal adduct formation (≤ 12%). RI ranged from 938 to 1145 and increased systematically with alkyl substitution, enabling discrimination of structural isomers. The combined EI and PCI data provide reliable spectral fingerprints and retention behavior for amidine-based degradation products of A-series nerve agents. Conclusions This work establishes comprehensive GC–MS reference data for amidine-based degradation products associated with Novichok A-series nerve agents. The developed method enabled the successful identification of spiked amidine compounds in the 56th and 57th OPCW proficiency test (PT) samples. These results will enhance the capability of forensic and verification laboratories to detect, confirm, and interpret degradation markers, thereby supporting OPCW PT, chemical weapons attribution, and compliance with the CWC.

(RCM) Characterization of Amidine‐Based Degradation Products of Novichok A‐Series Nerve Agents Using Single‐Quadrupole GC–MS: ABSTRACT




Rationale




The identification of Novichok A-series nerve agents depends primarily on the detection of stable… #RapidCommunMassSpectrom #MassSpecRSS

Small extracellular vesicles in clinical Cancer research - A quantitative proteomics perspective Publication date: Available online 8 March 2026 Source: Journal of Proteomics Author(s): Divya Pandey, Vineeta Tiwari, Dipanjana Ghosh

(J Proteom) Small extracellular vesicles in clinical Cancer research - A quantitative proteomics perspective: Publication date: Available online 8 March 2026

Source: Journal of Proteomics

Author(s): Divya Pandey, Vineeta Tiwari, Dipanjana Ghosh #MassSpecRSS

Simultaneous Quantification of Eight Chiral and Achiral Components in Notopterygii Rhizoma et Radix Extract and Rat Plasma Based on Chiral Stationary Phase‐HPLC‐MS/MS ABSTRACT A chiral stationary phase-high performance liquid chromatography-tandem mass spectrometry (CSP-HPLC-MS/MS) approach was developed and validated for the first time to quantify quantification of eight components: the enantiomers of three chiral components notopterol, oxypeucedanin hydrate, oxypeucedanin (in total six configurations), and achiral components nodakenin, imperatorin, isoimperatorin, bergapten, and ferulic acid, in Notopterygii Rhizoma et Radix. By adjusting the types of chiral stationary phase and the composition ratio of the mobile phase, methanol–acetonitrile (75:25, v/v) was selected as the mobile phase (flow rate 0.5 mL/min), and three chiral components notopterol, oxypeucedanin hydrate, and oxypeucedanin were successfully separated into their enantiomers with Chiralpak IG. The method was applied to analyze both plant extracts and rat plasma samples. A considerable variation in content was observed among the eight components in the plant extracts, with their individual concentrations covering a wide range from 1.04 to 20 400 µg/mL (see Section 3 for details). Similarly, their plasma concentrations also spanned from 0.2 to 262.76 ng/mL. The results demonstrated significant differences in the contents of the components. Notably, the chiral components exhibited marked differences between their enantiomers, suggesting that chiral components should be considered in the quality assessment and control of natural products.

(J Sep Sci) Simultaneous Quantification of Eight Chiral and Achiral Components in Notopterygii Rhizoma et Radix Extract and Rat Plasma Based on Chiral Stationary Phase‐HPLC‐MS/MS: ABSTRACT




A chiral stationary phase-high performance liquid chromatography-tandem mass… #JSepSci #MassSpecRSS

(Clin Chim Acta) Performance evaluation of automated magnetic beads extraction method for the measurement of 23 plasma steroids using dual liquid chromatography-tandem mass spectrometry (LC-MS/MS) method: Publication date: Available online 7 March 2026

Source: Clinica… #ClinChemActa #MassSpecRSS

(J Proteom) Transmutation learning: Breaking the reference database barrier to unlock single-cell proteomics: Publication date: Available online 7 March 2026

Source: Journal of Proteomics

Author(s): Aline A.M. Martins, Reynaldo Magalhães Melo, Lucas Sales, Marlon Dias Mariano dos… #MassSpecRSS

An Automated HDX-MS Platform for in situ characterisation of Membrane Proteins The structural study of membrane proteins has traditionally relied on detergent-based extraction from cellular membranes. Although native-like reconstitution approaches have advanced, fully understanding membrane protein dynamics requires examining them within their native membrane environment. Hydrogen-deuterium exchange mass spectrometry (HDX-MS) is a powerful method for probing structural dynamics in reconstituted systems, but the presence of the lipid bilayer introduces considerable complexity, limiting broader adoption under physiological conditions. Here, we present the first fully automated HDX-MS platform incorporating a two-stage delipidation workflow. We applied this approach to monitor the dynamics of the ABC transporter MsbA in isolated inner membrane vesicles (IIMVs) from Escherichia coli through its ATPase cycle. IIMVs revealed distinct dynamic features within the nucleotide binding domains and substrate binding cavity, highlighting physiologically relevant motions not observed with detergent solubilised MsbA. This platform significantly advances HDX-MS and underscores the importance of studying membrane proteins in native lipid environments.

(BioRxiv All) An Automated HDX-MS Platform for in situ characterisation of Membrane Proteins: The structural study of membrane proteins has traditionally relied on detergent-based extraction from cellular membranes. Although native-like reconstitution approaches have advanced,… #BioRxiv #MassSpecRSS

A multi-omics comparison unveils convergent and divergent antidepressant mechanisms of fluoxetine and St. John's wort extract Publication date: Available online 6 March 2026 Source: Journal of Proteomics Author(s): Jiale Zhang, Yuhang Cai, Panpan Zhang, Xurui Hao, Bowen Guo, Wanning Zhang, Yiying Wu, Jiawen Sun, Xiang Xu, Wanting Li, Bowen Zhang, Shuai Zhang, Wei Zhang, Dezhi Kong

(J Proteom) A multi-omics comparison unveils convergent and divergent antidepressant mechanisms of fluoxetine and St. John's wort extract: Publication date: Available online 6 March 2026

Source: Journal of Proteomics

Author(s): Jiale Zhang, Yuhang Cai, Panpan Zhang, Xurui Hao, Bowen… #MassSpecRSS

[ASAP] Oligomer-Dependent Gas-Phase Dissociation Behavior of 2-Butanone Peroxide (MEKP) Cations Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00450

(JASMS) [ASAP] Oligomer-Dependent Gas-Phase Dissociation Behavior of 2-Butanone Peroxide (MEKP) Cations: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00450 (RSS) #MassSpecRSS #JASMS

(J Chrom A) Characterization of siRNA Product-Related Impurities by Hydrophilic Interaction Liquid Chromatography (HILIC) Coupled with Mass Spectrometry: Publication date: Available online 5 March 2026

Source: Journal of Chromatography A

Author(s): Micaela Graglia, Sean Brian… #JChrom #MassSpecRSS

[ASAP] Exploring the Impact of Two DNA Minor Groove Binder Compounds on HCT-116 Cells: A Comprehensive Multiomics Analysis Using Mass Spectrometry Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.5c01039

(J Proteom Res) [ASAP] Exploring the Impact of Two DNA Minor Groove Binder Compounds on HCT-116 Cells: A Comprehensive Multiomics Analysis Using Mass Spectrometry: Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.5c01039 #MassSpecRSS

Identification of Quality Markers in Viola kunawaresis Royel Using HPLC Fingerprints, Chemometric Analysis, and Network Pharmacology ABSTRACT Violae tianshanicae herba (VTH), a widely used crude drug in Uyghur medicine in China, is renowned for its immunomodulatory, anti-inflammatory, antibacterial, antiviral, and antidiabetic activities, with notable efficacy in treating asthma. However, systematic research on its quality markers (Q-markers) remains limited, underscoring the need to analyze its chemical composition to enable effective quality control. A high-performance liquid chromatography (HPLC) fingerprint method was developed for 21 VTH batches in order to identify the common peaks of 23 flavonoids and esculetin, which were verified using liquid chromatography-mass spectrometry (LC–MS) and reference standards. In this study, chemometric methods were employed to differentiate samples from four geographical origins and to screen for differential components. In addition, a thorough examination of virtual target prediction in network pharmacology, in conjunction with molecular docking validation, has identified Q-markers with potential antiasthmatic effects. A group of six representative Q-markers (including kaempferol 3-O-sophorosyl-7-O-glucoside, esculetin, kaempferol 3-O-rutinosyl-7-O-glucoside, nicotiflorin, narcissoside, and astragalin) was selected for the purpose of developing a method to quantitatively analyze these components simultaneously in VTH samples. This study provides important references for the comprehensive quality control of VTH and establishes the foundation for researchers to explore the chemical basis and mechanisms of action of VTH.

(Biomed Chrom) Identification of Quality Markers in Viola kunawaresis Royel Using HPLC Fingerprints, Chemometric Analysis, and Network Pharmacology: ABSTRACT




Violae tianshanicae herba (VTH), a widely used crude drug in Uyghur medicine in China, is renowned for its… #massSpecRSS #biomedchrom

[ASAP] Hydrogen–Deuterium Exchange Mass Spectrometry Reveals an Antibody-Induced Allosteric Pathway Driving SARS-CoV-2 Spike Trimer Disassembly Journal of the American Chemical SocietyDOI: 10.1021/jacs.5c18784

(J Am Chem Soc) [ASAP] Hydrogen–Deuterium Exchange Mass Spectrometry Reveals an Antibody-Induced Allosteric Pathway Driving SARS-CoV-2 Spike Trimer Disassembly: Journal of the American Chemical SocietyDOI: 10.1021/jacs.5c18784 #JAmChemSoc #MassSpecRSS

(J Proteom) Editorial Board: Publication date: 20 April 2026

Source: Journal of Proteomics, Volume 326

Author(s): #MassSpecRSS

Comparison of extracellular vesicles and mechanically induced vesicles for structure determination of membrane proteins The structural and functional characteristics of membrane proteins can be influenced by the composition of the membrane. Consequently, native membranes are most relevant for the study of receptors and other membrane proteins. In this study, we investigated two types of cell-derived vesicles: natively shed extracellular vesicles (EVs) and mechanically derived vesicles (MVs). To this end, we utilized the human breast cancer cell line SKBR3, which strongly overexpresses the receptor HER2. We designed a protocol based on designed ankyrin repeat proteins (DARPins) to purify EVs and MVs enriched in HER2, and to ensure the native orientation of the HER2 receptors within the vesicle. The isolated HER2-containing EVs and MVs were characterized by cryo-EM, cryo-electron tomography (cryo-ET) and mass spectrometry (MS), which revealed fundamental differences between the different vesicle types. Our study highlights the greater structural diversity of EVs over MVs. A single particle cryo-EM analysis and classification of all visible receptors on the vesicle surface yielded electron density consistent with HER2 at modest resolution. Taken together, our results suggest that MVs can serve better than EVs as a suitable platform for the structure determination of membrane proteins within their native membrane environments.

(BioRxiv All) Comparison of extracellular vesicles and mechanically induced vesicles for structure determination of membrane proteins: The structural and functional characteristics of membrane proteins can be influenced by the composition of the membrane. Consequently, native… #BioRxiv #MassSpecRSS

(Angew Chem) Dynamic Decoupling of Pt─H Intermediates Formation From Water Dissociation for Efficient Alkaline Hydrogen Evolution: Conventional platinum-based catalysts for alkaline hydrogen evolution reaction (HER) are hindered by the sluggish water dissociation,… (RSS) #AngewChem #MassSpecRSS

Salt‐Regulated Confinement of FeO Microcrystallites on Amorphous Mn3CoOx for Boosting Sustainable Acidic Water Oxidation ABSTRACT Nanomaterials with amorphous surface have attracted significant attention in the oxygen evolution reaction (OER), which still needs further investigations. In this work, we developed a novel Salt-regulated confinement loading method to prepare amorphous Mn3CoOx support confined FeO microcrystallites at a relatively low-temperature (623 K). The confined FeO microcrystallites showed strong interfacial electronic interactions with Mn3CoOx matrix (abundant defect sites and flexible local environments), enabling efficient charge transfer and enhanced intermediate stabilization for efficient OER in acidic media. The FeO/Mn3CoOx exhibits remarkable OER performance, with a low overpotential of 252 mV@10 mA cm−2 with a significantly lower Tafel slope of 79 mV dec−1, outperforming the commercial IrO2 (∼ 290 mV@10 mA cm−2). Mechanistic studies reveal that the incorporation of FeO microcrystallites, as electron reservoirs to stabilize high-valence intermediates and facilitate continuous turnover, induces a synergistic transition from a purely lattice oxygen-mediated mechanism (LOM) to a dual LOM and oxygen pathway mechanism (OPM).These results are well corroborated by in situ attenuated total reflection surface-enhanced infrared spectroscopy, differential electrochemical mass spectrometry, and density functional theory calculations. Our work provides a robust strategy to design amorphous, non-precious-metal OER catalysts capable of stable operation in acidic media, offering a scalable route toward efficient hydrogen production.

(Angew Chem) Salt‐Regulated Confinement of FeO Microcrystallites on Amorphous Mn3CoOx for Boosting Sustainable Acidic Water Oxidation: ABSTRACT




Nanomaterials with amorphous surface have attracted significant attention in the oxygen evolution reaction (OER), which… (RSS) #AngewChem #MassSpecRSS

Metabolite Profiling and Identification of Semaglutide in Liver S9 Across Species and Rat Plasma ABSTRACT Semaglutide, a glucagon-like peptide-1 receptor agonist, stands as a paradigm of successful peptide drug development. Although absorption, distribution, metabolism and excretion characteristics of semaglutide have been thoroughly studied in both animals and humans, a reliable in vitro screening model for evaluating metabolic behavior of semaglutide and other peptides remains an unmet need in drug development. This study established a novel ultrahigh performance liquid chromatography coupled to high-resolution mass spectrometry method to elucidate the species-specific metabolic pathways of semaglutide. Semaglutide was incubated with liver S9 fractions from human, monkey, dog, rat, and mouse for 1 h, respectively. For the in vivo study, rats were subcutaneously injected with semaglutide (10 mg/kg) for subsequent plasma collection. Data-dependent acquisition was performed to obtain the fragment ions, enabling identification of drug-related materials. Notably, a diagnostic fragment ion at m/z 469, formed from the cleavage of side chain, facilitated metabolite screening. Consequently, a total of 31 metabolites were detected and characterized, constituting a comprehensive metabolic profile of semaglutide in liver S9 fractions and rat plasma. Major metabolic pathways involved hydrolysis of peptide backbone. Our findings provided a robust methodological framework for the screening and metabolism prediction of peptide candidates, supporting the comprehensive safety and efficacy assessment.

(Biomed Chrom) Metabolite Profiling and Identification of Semaglutide in Liver S9 Across Species and Rat Plasma: ABSTRACT




Semaglutide, a glucagon-like peptide-1 receptor agonist, stands as a paradigm of successful peptide drug development. Although absorption,… #massSpecRSS #biomedchrom

(Analyst) Study on low temperature enrichment-headspace sampling-proton transfer reaction-mass spectrometry (LTE-HS-PTR-MS) for highly sensitive analysis of trace exhaled VOCs: Analyst, 2026, Advance Article
DOI: 10.1039/D6AN00175K, PaperJie Jin, Wei Xu, Ning Zhang, Qi… (RSS) #MassSpecRSS #analyst

Early Identification of At-Risk Patients: ProteomicSignature Predicts Progression to DecompensatedCirrhosis Background & Aims: Early identification of decompensation in patients with cirrhosis is important to enable timely detection, management of complications and for effective treatment. This study investigates the biology of decompensation and aim to identify protein biomarkers for identification of high-risk patients. Methods: The primary analysis included plasma samples from 46 patients with metabolic dysfunction associated steatotic liver disease (MASLD) related cirrhosis. Plasma samples were depleted for the top 14 most abundant proteins and the proteome was measured by liquid chromatography tandem mass spectrometry. The dataset was divided into a training (14 compensated, 10 decompensated) and a test cohort of compensated patients (11 progressing to decompensation, 11 remaining compensated). Changes in protein levels were determined by ANCOVA and a prognostic model was developed using logistic regression. External validation was performed in an independent cohort of 120 patients with alcohol-related cirrhosis. Time-to-event analyses were conducted in this cohort using Cox regression. Results: 52 proteins involved in impaired hepatic function, fibrogenesis, immune activation, and metabolic changes were significantly different between compensated and decompensated patients. A prognostic model with four proteins (NBL1, LTBP4, APOC4, GHR), demonstrated predictive ability for future decompensation (AUC=0.93, 73% sensitivity, 100% specificity). In the external validation cohort, the model demonstrated generalizability (AUC=0.78, 72% sensitivity, 82% specificity). Validation cohort time-to-event analyses showed that higher baseline scores were associated with shorter time to liver-related events (HR 1.32; log-rank p = 0.027), underscoring the panel's prognostic value. Conclusion: Our study indicates that patients with decompensated cirrhosis are characterized by proteomic signatures of fibrogenesis and metabolic dysfunction. Capturing these signatures could help identify patients at risk of complications and potentially those eligible for aetiology directed treatment.

(BioRxiv All) Early Identification of At-Risk Patients: ProteomicSignature Predicts Progression to DecompensatedCirrhosis: Background & Aims: Early identification of decompensation in patients with cirrhosis is important to enable timely detection, management of complications… #BioRxiv #MassSpecRSS

B-Lactamase-Responsive Probe for Rapid and Non-invasive Detection of Tuberculosis through Induced Breath Analysis Pulmonary tuberculosis (PTB) remains a major global public health challenge. Existing diagnostic approaches are generally limited by suboptimal sensitivity, prolonged turnaround times, high costs, and reliance on sputum samples. These constraints hinder large-scale implementation in resource-limited settings and substantially impede early screening and timely intervention. Targeting the characteristic expression of {beta}-lactamase in Mycobacterium tuberculosis, this study employed systematic screening of a molecular compound library and identified cefapirin sodium as a specific molecular probe. Upon selective hydrolysis by {beta}-lactamase, the probe releases hydrogen sulfide (H2S), which can be detected using a highly sensitive H2S sensing system, thereby enabling rapid and noninvasive identification of the pathogen. Gas chromatography-mass spectrometry (GC-MS) and colorimetric assays were used to validate the specificity of the enzymatic reaction and to confirm the accuracy of the generated product, collectively demonstrating the feasibility of the proposed detection platform. This method offers several advantages, including noninvasiveness, rapid response, and low cost, making it particularly suitable for application in resource-constrained regions. It provides a promising new strategy for early, point-of-care, and large-scale screening of pulmonary tuberculosis, with important implications for improving TB control efforts in high-burden settings.

(BioRxiv All) B-Lactamase-Responsive Probe for Rapid and Non-invasive Detection of Tuberculosis through Induced Breath Analysis: Pulmonary tuberculosis (PTB) remains a major global public health challenge. Existing diagnostic approaches are generally limited by suboptimal… #BioRxiv #MassSpecRSS

(J An Atom Spec) HCl-selective ionization reactions for improved Cl detection robustness in post-inductively coupled plasma chemical ionization: J. Anal. At. Spectrom., 2026, Accepted Manuscript
DOI: 10.1039/D5JA00483G, Paper Open Access &nbsp This article is licensed… #jaas #MassSpecRSS #AtomicSpec

[ASAP] Integrating Ion Beam Control into a Commercial Platform for Improved Multimodal SIMS/MALDI Imaging Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00423

(JASMS) [ASAP] Integrating Ion Beam Control into a Commercial Platform for Improved Multimodal SIMS/MALDI Imaging: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00423 (RSS) #MassSpecRSS #JASMS

[ASAP] Interpretable Machine Learning to Decipher Myelodysplastic Syndrome-Associated Alterations of the Extracellular Matrix by Time-of-Flight Secondary Ion Mass Spectrometry Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00343

(JASMS) [ASAP] Interpretable Machine Learning to Decipher Myelodysplastic Syndrome-Associated Alterations of the Extracellular Matrix by Time-of-Flight Secondary Ion Mass Spectrometry: Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.5c00343 (RSS) #MassSpecRSS #JASMS

Total Breakthrough Strategy in Hydrophilic Interaction Chromatography for IP‐RPLC × HILIC Separation of Oligonucleotides ABSTRACT Solvent mismatch between the two dimensions is one of the main limitations in two-dimensional liquid chromatography (2D-LC) and presents a significant challenge for method development. Although 2D-LC provides a powerful means to increase peak capacity for oligonucleotides analysis compared to conventional one-dimensional LC, solvent incompatibility remains a major obstacle that discourages broader development of such methods. In this work, we investigate a technical solution that can be easily implemented and that eliminates solvent mismatch effects during 2D-LC analysis of oligonucleotides. This approach is based on the total breakthrough behavior of oligonucleotides, which is a phenomenon that allows the injection of large volumes into the second dimension (2D) without peak distortion. In this work, we showed that under appropriate conditions, oligonucleotides exhibit total breakthrough behavior in HILIC. This behavior in HILIC is particularly advantageous, as the IP-RPLC × HILIC configuration offers improved mass spectrometry (MS) compatibility compared to IP-RPLC or HILIC × IP-RPLC. Assuming an IP-RPLC × HILIC configuration, we systematically investigated the composition of first-dimension (1D) fractions and the 2D-HILIC parameters influencing total breakthrough to identify the key factors promoting this behavior. Our results offer clear guidance for implementing successful IP-RPLC × HILIC conditions that avoid mismatch effects for oligonucleotides analysis while maintaining a high injection volume in the second dimension. This work demonstrates the potential of the total breakthrough strategy for implementing 2D-LC methods with HILIC as the second dimension.

(J Sep Sci) Total Breakthrough Strategy in Hydrophilic Interaction Chromatography for IP‐RPLC × HILIC Separation of Oligonucleotides: ABSTRACT




Solvent mismatch between the two dimensions is one of the main limitations in two-dimensional liquid chromatography (2D-LC) and… #JSepSci #MassSpecRSS

Spatial selection of ions separated in a multi-reflection time-of-flight mass analyzer Publication date: Available online 5 March 2026 Source: International Journal of Mass Spectrometry Author(s): Mikhail I. Yavor, Kirill A. Koval

(IJMS) Spatial selection of ions separated in a multi-reflection time-of-flight mass analyzer: Publication date: Available online 5 March 2026

Source: International Journal of Mass Spectrometry

Author(s): Mikhail I. Yavor, Kirill A. Koval #ijms #MassSpecRSS

Rewiring of 3D Enhancer-Promoter Interactome Underlies Diabetic Endothelial Dysfunction Background: Diabetic vascular complications are driven by endothelial dysfunction, yet the role of 3D genome organization in this process is unknown. We sought to define the alterations in chromatin architecture in diabetic endothelium and identify the key regulators involved. Methods: We generated a high-resolution 3D epigenomic atlas of diabetic endothelial cells from mouse models and human subjects using H3K27ac HiChIP, complemented by ChIP-seq, ATAC-seq, and RNA-seq. A human cohort was used to assess protein expression in diabetic versus non-diabetic endothelial cells. To identify JUNB-interacting proteins, we performed rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME), with protein-protein interaction validated by co-immunoprecipitation. Functional validation was performed using in vitro, ex vivo, and in vivo approaches, including endothelial-specific knockdown in a diabetic hindlimb ischemia model. Results: Multi-omics profiling revealed extensive enhancer reprogramming in diabetic endothelium, with AP-1 binding motifs being consistently and selectively enriched in downregulated enhancers across three distinct diabetic models. Analysis of a human cohort confirmed significantly reduced JUNB protein levels in diabetic endothelial cells. We identified widespread disruption of JUNB-anchored enhancer-promoter interactions, which underlies transcriptional repression of key endothelial genes. RIME and co-immunoprecipitation established the E3 ubiquitin ligase RBBP6 as a direct JUNB interactor that promotes its polyubiquitination and proteasomal degradation in response to hyperglycemia. Human cohort analysis further showed reciprocal elevation of RBBP6 in diabetic endothelial cells. Either JUNB overexpression or RBBP6 knockdown restored enhancer-promoter connectivity, reactivated vasoprotective transcriptional programs, and rescued endothelial function. Critically, endothelial-specific knockdown of Rbbp6 in diabetic mice restored endothelium-dependent vasorelaxation and improved perfusion recovery after hindlimb ischemia, independent of systemic glucose levels. Conclusions: Our study unveils a novel mechanism whereby hyperglycemia induces enhancer reprogramming and disrupts endothelial 3D genome architecture through RBBP6-mediated degradation of JUNB. The RBBP6-JUNB axis is established as a crucial link between metabolic stress and epigenomic reprogramming in vascular disease, presenting a promising therapeutic target for diabetic vasculopathy.

(BioRxiv All) Rewiring of 3D Enhancer-Promoter Interactome Underlies Diabetic Endothelial Dysfunction: Background: Diabetic vascular complications are driven by endothelial dysfunction, yet the role of 3D genome organization in this process is unknown. We sought to define the… #BioRxiv #MassSpecRSS

Cation‐Dependent Multilayered Host–Guest Assemblies Formed by a Xanthene‐Based Dinuclear Nickel(II) Macrocycle Chemistry – A European Journal, Volume 32, Issue 9, 2 March 2026.

(CEJ) Cation‐Dependent Multilayered Host–Guest Assemblies Formed by a Xanthene‐Based Dinuclear Nickel(II) Macrocycle: Chemistry – A European Journal, Volume 32, Issue 9, 2 March 2026. (/CEJ)

Endosome motility controls light-responsive reproductive development and secondary metabolite production in Aspergillus Filamentous fungi, such as Aspergillus species, use microtubule transport to move early endosomes. Other cargos, such as peroxisomes and mRNAs, "hitchhike" on early endosomes to move throughout the long hyphae of these organisms. In Aspergillus nidulans, peroxisomes hitchhike on early endosomes using the endosomal protein PxdA and the peroxisomal protein AcbdA. The HookA adaptor protein links endosomes to microtubule motors. Here, we set out to explore the physiological functions of peroxisome hitchhiking and endosome motility. A. nidulans has a complex life cycle that includes asexual and sexual reproduction. A. nidulans and other fungi within the Pezizomycotina subphylum are also notable for the vast number of secondary metabolites they produce. Light and other environmental conditions influence developmental decisions and secondary metabolite production. Here, we found that sexual reproduction is favored in the absence of endosome motility, even in the light, which normally promotes asexual reproduction. RNA sequencing of strains lacking endosome motility showed altered expression of genes involved in development. Unexpectedly, we observed altered expression of genes involved in secondary metabolism in strains lacking endosome motility and peroxisome hitchhiking. Using mass spectrometry, we found that the loss of endosome motility affected the biosynthesis of secondary metabolites, including sterigmatocystin, a carcinogenic mycotoxin that is a food contaminant. Finally, in a pathogenic species, Aspergillus fumigatus, we found that deletion of its PxdA homolog also significantly altered secondary metabolite production. Our work uncovers an unexpected link between organelle motility, developmental decisions in response to light, and secondary metabolite production in filamentous fungi.

(BioRxiv All) Endosome motility controls light-responsive reproductive development and secondary metabolite production in Aspergillus: Filamentous fungi, such as Aspergillus species, use microtubule transport to move early endosomes. Other cargos, such as peroxisomes and… #BioRxiv #MassSpecRSS

(Angew Chem) Breaking Activity‐Stability Trade‐Off by Switching Reaction Pathway for Efficient Electrosynthesis of Glycerate at Industrial‐Scale Current Density: Benefiting from modulated electronic structure of glyceraldehyde intermediate, which stabilizes C─C bond… (RSS) #AngewChem #MassSpecRSS

Direct Oxidation of Methane to Formaldehyde With Molecular Oxygen Catalyzed by Gold‐Tungsten Oxide Cluster Cations The cluster catalyst, AuWO2 +, being able to catalyze the reaction of CH4 + O2 → CH2O + H2O at room temperature has been successfully identified. The Au−W center undergoes thermal reaction with O2 to generate the (O···O)−• species that works together with the Au atom in a relay-manner to directionally cleave two C−H bonds of methane for CH2O and H2O production. ABSTRACT Catalytic direct oxidation of methane with molecular oxygen can be highly exothermic in thermodynamics. It offers a promising green method for production of value-added chemicals such as formaldehyde (CH2O), an indispensable feedstock in industry, under mild conditions. However, it faces a long-standing grand challenge due to the intrinsic kinetic inertness of methane. Herein, an active gold-tungsten oxide cluster catalyst, AuWO2 +, being able to spontaneously catalyze CH4 + O2 → CH2O + H2O at room temperature has been successfully identified by mass spectrometry, which is distinctly different from the related condensed phase catalysis wherein the photo-excitation of catalysts in the presence of H2O or H2 was prerequisite to initiate catalytic reactions. The previously unrecognized mechanisms of direct methane oxidation have been unveiled: the interfacial Au−metal centers can undergo thermal reaction with O2 to spontaneously generate the active (O···O)−• hole that subsequently works together with Au atom in a relay-manner to easily cleave two C−H bonds of methane directional for CH2O and H2O production. This finding lays a solid foundation for future design of better-performing catalysts for direct oxidation of methane (or other molecules) with O2 without the need of any external energy or co-feeding with alien molecules.

(Angew Chem) Direct Oxidation of Methane to Formaldehyde With Molecular Oxygen Catalyzed by Gold‐Tungsten Oxide Cluster Cations: The cluster catalyst, AuWO2
+, being able to catalyze the reaction of CH4 + O2 → CH2O + H2O at room temperature has been successfully… (RSS) #AngewChem #MassSpecRSS