Makoto T Hayashi

Thrilled to see our work published! Huge thanks to all my co-authors for the great collaboration!!

10/10 For a more detailed explanation, check out bsky.app/profile/thec... for an in-depth thread on our findings.

9/10 These insights not only advance our understanding of telomere biology but also opens potential therapeutic avenues for targeting mitotic death in cancer cells.

8/10 Our findings support a dynamic model where coordinated post-translational modifications of shelterin promote t-loop unwinding, aiding in the removal of damaged cells from the cell cycle.

7/10 This phosphorylation is essential for BTR-mediated double Holliday junction dissolution and contributes to mitotic telomere deprotection, highlighting a previously underappreciated role of the shelterin complex.

6/10 Shelterin is a telomere-binding protein complex that includes TRF1 and TRF2, crucial for maintaining telomere structure and function. During mitotic arrest, Aurora Kinase B (AURKB) of the CPC phosphorylates TRF1 and TRF2, leading to telomere linearity and a telomere-specific DDR.

5/10 Our study uncovers a sophisticated mechanism involving the Chromosome Passenger Complex (CPC), the shelterin complex, and the BLM-TOP3A-RMI1/2 (BTR) complex in regulating MAD-telomere deprotection.

4/10 Telomeres safeguard chromosome ends by forming t-loops, preventing ATM activation and unwarranted DNA damage responses (DDR). However, during mitotic arrest, telomere linearity and a localized DDR emerge—a phenomenon known as "Mitotic Arrest Dependent (MAD)-Telomere Deprotection."

3/10 Additional key contributors include Ronnie Low, Scott Page, Blake Lane, Andrew Robinson, and Lucy French from @thecesarelab.bsky.social. Fuyuki Ishikawa from Kyoto University and Shunya Kosaka from my lab.

2/10 This work highlights the power of collaboration. Special thanks to our fantastic collaborator @thecesarelab.bsky.social. Diana Romero from my lab and Sam Rogers from Cesare’s lab contributed equally to this work. Their perseverance truly deserves immense recognition!

A CPC-shelterin-BTR axis regulates mitotic telomere deprotection Nature Communications - Here the authors reveal how telomeres signal mitotic stress. A key protein network alters their structure exposing telomere ends to signal mitotic stress, ultimately...

🧵 1/10 Excited to share our latest study in @naturecomms.bsky.social
"A CPC-shelterin-BTR axis regulates mitotic telomere deprotection" t.co/VypPsLBJCR