Sergio Cruz-León

The scientific part of the event starts with a talk by @sergiocruzleon.bsky.social on bridging in-situ cry-ET and multi scale simulations

We resolved the molecular architecture of a condensate—inside cells. 🤯 By combining in situ cryoET with multiscale simulations, we show how a single point mutation modifies molecular interactions, shifts the condensate’s material properties, and alters its biological function. Preprint 👇

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

We developed a 'smart STED microscope' to detect rare objects/events in cells, and unraveled the 3D morphology of a nuclear membraneless organelle (paraspeckle). Amazing collaboration with Sjoerd Stallinga, Bernd Rieger (TU Delft) and @mixmue.bsky.social 🙏 (1/3) www.nature.com/articles/s41...

Just published! We share an open-source workflow to measure membrane thickness from tomograms, including a tutorial with 3D visualizations. We analyze thickness variations across organelles and reflect on where to define a membrane boundary. @becklab.bsky.social #teamtomo rupress.org/jcb/article/...

Structure of the ribosome-bound SND3 translocon complex from the heat-tolerant fungus Chaetomium thermophilum. The SND3 translocon inserts proteins into the endoplasmic reticulum membrane as they are produced by the ribosome.

SNDing proteins into the membrane! Our new publication from @melaniemcdowell.bsky.social ’s group identifies the SND3 protein as a new route for membrane protein insertion! 🍄 📘 Read more here: www.mpg.de/25599408/102... Image: Louise Duever.

cryoTIGER: deep-learning based tilt interpolation generator for enhanced reconstruction in cryo electron tomography - Communications Biology Deep learning-based interpolation using cryoTIGER enhances angular sampling in cryoET, improving 3D reconstructions, particle localization, and structural recovery without increasing electron dose.

Deep learning-based interpolation using cryoTIGER developed by the authors in this new Communications Biology manuscript enhances cryoET reconstructions. @mpibp.bsky.social
@becklab.bsky.social
@jpkreysing.bsky.social
@sergiocruzleon.bsky.social

doi.org/10.1038/s420...

Photo of Sergio Cruz and text: Sergio Cruz-León receives the 2026 Theory & Computation Postdoctoral Research Award

🎉 Congratulations to our postdoc @sergiocruzleon.bsky.social for receiving the 2026 Theory & Computation Postdoctoral Research Award from the Biophysical Society! 🎉 Sergio will present his work at the Theory & Computation subgroup Symposium during the 2026 Biophysical Society meeting.

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That's a wrap! The results of the first #cryoEM heterogeneity challenge are up on biorxiv!
biorxiv.org/content/10.110

Proud to share our first lab pre-print: “SND3 is the membrane insertase within a fungal multipass translocon” where @tzujingyang.bsky.social solved the structure of a ribosome-associated SND3-translocon complex involved in ER membrane protein insertion ➡️ doi.org/10.1101/2025...

Thanks 😀🧬

🧬😄! Thanks!

😄 thank you!

😄 thanks!

Thanks!

Thanks!

Thanks!

Thanks!

New preprint on 3D heterochromatin architecture in human cells! Great collab with @sergiocruzleon.bsky.social & @johannesbetz.bsky.social from @hummerlab.bsky.social, @marinalusic.bsky.social & the Turoňová lab. Many thanks to my supervisor @becklab.bsky.social. bioRxiv: tinyurl.com/3a74uanv 🧵👇

Excited to share our preprint on the molecular architecture of heterochromatin in human cells 🧬🔬w/ @jpkreysing.bsky.social, @johannesbetz.bsky.social,
@marinalusic.bsky.social, Turoňová lab, @hummerlab.bsky.social @becklab.bsky.social @mpibp.bsky.social

🔗 Preprint here tinyurl.com/3a74uanv

Our paper on prediction of phase-separation propensities of disordered proteins from sequence is now published:
www.pnas.org/doi/10.1073/...

The paper has been substantially updated compared to the preprint including new experimental data and using the neural network to finetune CALVADOS. 1/n

✨✨ It's #glycotime for #HIV Env ✨✨

N-linked glycans modulate flexibility & MPER epitope exposure #glycotime

Huge effort by @shehata92.bsky.social @lcasalino88.bsky.social with cryoET of Env in VLPs by @thevillalab.bsky.social & team 💪

Would love feedback!

www.biorxiv.org/content/10.1...

Max Planck Research Group Leader (W2) in Molecular Design We are looking for exceptional early-career scientists conducting computational research with a proven record of accomplishment. The primary focus of this call is on candidates proposing research on b...

Job alert: Join us in Mainz as

Max Planck Research Group Leader (W2) in Molecular Design

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@mpip-mainz.mpg.de

Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...

Nuclear pores safeguard the integrity of the nuclear envelope Nature Cell Biology - Taniguchi et al. structurally analyse nuclear pore complex architecture in situ during differentiation, which is associated with mechanical constraints on the nuclear...

Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9
Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳

Happy to share our manuscript on the in situ visualization of the copia retrotransposon in its final form today published in @cellcellpress.bsky.social www.cell.com/cell/fulltex.... What’s new?

Interested in #RNA modelling across multiple scales? Join us for the @cecamevents.bsky.social meeting at SISSA, Trieste, May 19-22! www.cecam.org/workshop-det... We still have slots for contributed talks. Deadline March 23. Co-organized with @magistratolab.bsky.social and @marcodevivo.bsky.social

🤩 Highly optimized software running on a SOA #HPC cluster provides sufficient #computing power to #simulate #biomolecular systems at the mesoscale of viruses and organelles, and potentially small cells in the near future... Want to know more? Check out our paper 📑 in #JCC 🔗 doi.org/10.1002/jcc....

Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4