Alessandro Donada

The #ISEH2025 🏯 program is here and features world-renowned scientists showcasing the latest in experimental hematology. Don't miss these sessions, including the ISEH/JSH symposium on stress hematopoiesis with Drs. Toshio Suda and Hitoshi Takizawa ➡️ www.iseh.org/2025Scientif...

So interesting!

A gentleman and a scholar indeed! What a pleasant chat with @abattacks.bsky.social, make sure to check out our interview! Also, a submitted post from @lvchosen1.bsky.social and at least 25 preprints to review! #hemesky

Let's do this!

Best way to keep up with the newest research! #HemeSky

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This project was led with @perielab.bsky.social, with the help of amazing collaborators — shoutout to Gurvan, CSCO, Tiziana, and the clinicians.

Want to know more? Send me a message, or comment here!

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🔮 Where are we headed next?

Can we restore epigenetic structure in AML more robustly?

How stable is clonal memory over time, stress, or environment?

Let’s find out.

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🧨 In AML, the memory breaks down.
→ Cell families get chaotic
→ Division becomes erratic
→ Concordance is lost
But: tweaking the epigenetic landscape brings some order back.
🙏 Huge thanks to patients & clinicians who made this part possible.

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🧬 What about gene expression?

No one-size-fits-all “family module,” but expression fingerprints are shared within families. And I want to thank the @CSCO platform at Curie for the data & Tiziana Tocci for the beautiful network analysis!

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📐 Could this all be random?
We built a mathematical model (thanks to Gurvan Hermange) to test it.
Still:
✅ Homogeneity in fate
✅ Concordance in division
...emerged as essential features.

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🧬 It’s not just division.
Cell families also:
→ Look alike
→ Choose similar fates
The big differences are between families, not within.
🧭 Lineage tracing was key.

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👪 Cells from the same ancestor — “families” — often:
✔️ Divide the same number of times
✔️ At the same time ⏱️
And this holds across donors, media & time.

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🔬 We took a multi-omic, single-cell approach:

Live-cell imaging

Single-cell RNA-seq (thanks CSCO platform!)

Custom assay for lineage + division + differentiation --> introducing MultiGen (DOI: 10.3791/64918)

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Why HSPCs? 🩸
These stem cells build the entire blood system.
If they remember division patterns, it affects how we understand both healthy haematopoiesis and disease.

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Cells divide, differentiate, and move on… right?
🧠 But we asked: do blood stem cells remember their past divisions?
Spoiler: they do — and it’s surprisingly organized.

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🧬 What if your blood cells remembered how their ancestors divided?

Our new preprint (www.biorxiv.org/content/10.1...) explores clonal memory in human blood stem cells — and how it breaks down in leukaemia.

📄 "Clonal memory of cell division..."

This Article analyzes human hematopoiesis in humanized bone marrow in immune-deficient mice, showing dynamic structures supporting human hematopoietic development. Findings emphasize significance of the models for investigating human hematopoietic development & leukemogenesis👉https://bit.ly/42Czbaw

🚨🚨 We're hiring! 🚨🚨
The Single-cell Analytics Innovation Lab (SAIL) at @mskcancercenter.bsky.social is looking for a Junior Technician to join our wet lab team!
You'll work with spatial genomics and single-cell technologies, helping us push the boundaries of cutting-edge analysis in cancer research.

What are you waiting for? Best society you can be involved in!

Such a beautiful example of how little we know and understand about biology! In my Top3 together with tardigrades and the naked mole rat!

Make him pay ladies. And a lot of respect for a postdoc taking this type of stance, we can only hope to be like that.

Invited speaker @trowbridgelab.bsky.social‬ will share her work this September at #ISEH2025 🏯 Learn more about the incredible science to expect this year and register today! youtu.be/RnyyurVaZrc

ISEH Committees - International Society for Experimental Hematology

Calls for volunteers to serve on a variety of committees for the @isehsociety.bsky.social are open now! Check out these opportunities if you're interested in developing leadership skills and your professional network. It takes a village! www.iseh.org/page/ISEHCom...

Find 20 minutes of your time and listen to this interview, plenty of valuable insights!

That's a brilliant interview, with some genuine takes on this entire business of understanding blood cells :)

Cell-extrinsic factors contribute toward myeloid-biased expansion in Tet2-mutant clonal hematopoiesis Hematopoietic stem cells (HSCs) acquire somatic mutations throughout life, with the frequency of mutational incidence increasing with age [1]. Occasionally, certain mutations confer a fitness advantag...

Excited to see the first commentary in Experimental Hematology (@isehsociety.bsky.social)! Kudos to Aishwarya, Linde ‪@lindemileslab.bsky.social, and Grant @challenlab.bsky.social for highlighting recent papers in Exp Hem on cell-extrinsic factors in Tet2-mutant clonal hematopoiesis!! bit.ly/4k3pEAI