Jonathan Coxhead

YouTube video by Richard J Murphy Britain’s property addiction: how politics has built a broken market

youtube.com/watch?v=PvkH...

YouTube video by Red Bull Bike TOP 3 RUNS from Men's Finals Red Bull Rampage 2025 I SPOILER ALERT!

Top three runs from men's finals - Red Bull Rampage 2025

youtu.be/9Rcf_uS8QTQ?...

Cardinal Black at the Wylam Brewery with Dan tonight - fantastic band, live 👏👏👏

📷 Chris Buck 🎸🧙🙇

🎵 Geese - Getting Killed 🔊🔊

Government U-turns on support for bird-friendly swift bricks in new homes Housing minister Steve Reed refuses to back mandating measure, despite giving support while in environment job

Exasperated. Gutted. Furious. Terrified. Sometimes swear words are the only ones that fit.
Our delightful government - who we put our trust in - is u-turning on THE easiest Nature action *ever*.
www.theguardian.com/environment/...

On the 'Keel Crossing' with Dan 😍 headed to the SoL.

#HawayTheLads ♥️🤍⚽ #SAFC

🛋️📺🍕🍷👍

Teaching finished for this year 🏌️🧬🧬

Evaluating the potential and limitations of nanopore adaptive sampling for targeted transcriptome sequencing. #LongRead #AdaptiveSampling #TargetedSequencing #Transcriptomics #Genomics @nanoporetech.com 🧬
genomebiology.biomedcentral.com/articles/10....

Environmental community transcriptomics: strategies and struggles Abstract. Transcriptomics is the study of RNA transcripts, the portion of the genome that is transcribed, in a specific cell, tissue, or organism. Transcri

Environmental community transcriptomics: strategies and struggles academic.oup.com/bfg/article/...

Job done @nigella.bsky.social - tonight's tea 😋

The promises and pitfalls of automated variant interpretation: a comprehensive review Abstract. The interpretation of DNA variants enables personalized medicine through precise diagnosis and treatment selection. To address the challenges of

The promises and pitfalls of automated variant interpretation: a comprehensive review academic.oup.com/bib/article/...

Impact of RNA extraction on respiratory microbiome analysis using third-generation sequencing - BMC Genomics The respiratory microbiome, which comprises bacteria, fungi, and viruses, plays a crucial role in respiratory health and disease. However, its study is limited by the low microbial biomass in respiratory samples and the dominance of host RNA. Metatranscriptomics offers comprehensive insights into active microbial communities and their interactions with the host but requires optimized RNA extraction protocols for robust and unbiased analysis. This study evaluated two RNA extraction kits—one employing chemical lysis (CL) and another combining chemical and mechanical lysis (CML)—to determine their effectiveness for metatranscriptomic analysis of respiratory samples. The CML protocol significantly increased double-stranded DNA (dsDNA) library yields, leading to higher sequencing read counts for both sample types (p < 0.0001). The read length was unaffected by the lysis protocol for the BAL and NPS samples. Taxonomic profiling revealed that CML enhanced the detection of robust microorganisms, such as gram-positive bacteria and fungi, without compromising viral detection. The CML protocol demonstrated superior recovery of genetic material, particularly for fungi and gram-positive bacteria, making it better suited for comprehensive metatranscriptomic analyses. These findings underscore the need for tailored RNA extraction strategies on the basis of sample type and research objectives. Optimized metatranscriptomic protocols are pivotal for advancing our understanding of the respiratory microbiome and its role in health and disease.

Impact of RNA extraction on respiratory microbiome analysis using third-generation sequencing bmcgenomics.biomedcentral.com/articles/10....

A scalable approach for genomic-first rare disorder detection in a healthcare-based population Beyond commonly screened disorders, genomic-first ascertainment of genetic disorders remains underexplored. We developed a scalable framework for 2,701 additional rare genetic disorders, identifying p...

A scalable approach for genomic-first rare disorder detection in a healthcare-based population www.cell.com/ajhg/abstrac...

The utility of ultra-deep RNA sequencing in Mendelian disorder diagnostics RNA sequencing has emerged as a powerful tool for Mendelian disorder diagnostics, but the optimized sequencing depth has not been determined. Using Ultra-high-depth RNA sequencing (up to 1 billion rea...

The utility of ultra-deep RNA sequencing in Mendelian disorder diagnostics www.cell.com/ajhg/abstrac...

Forensic genetics in the omics era - Nature Reviews Genetics Recent advances in forensic genetics have improved the range, precision and reliability of forensic information&nbsp;obtainable from biological trace material. The author reviews how non-targeted and targeted&nbsp;omics approaches and&nbsp;methods are improving crime scene analyses being applied for the identification of perpetrators and their relatives or victims, the prediction of phenotypic traits, and the determination of trace characteristics.

Review | Forensic genetics in the omics era www.nature.com/articles/s41...

NEW: The British politician, his Russian intelligence handler & a Kremlin plot against the US & Ukraine.

My new piece about Nathan Gill and Nigel Farage for @thenerve_news in which we ask:

Why, even now, is no-one asking questions?

t.co/BUTtpK9C4S

‘Verging on unwatchable’: Guardian writers on their most stressful movies With the US release of Rose Byrne’s anxiety-inducing motherhood spiral If I Had Legs I’d Kick You, writers relive their most uncomfortable movie watches

‘Verging on unwatchable’: Guardian writers on their most stressful movies

Do you ring batman often 😉

In praise of “rip-off” degrees | The Observer Kemi Badenoch’s attack on the arts and humanities isn’t just culturally short-sighted, it’s economically illiterate

Source: The Observer
search.app/QRu5a

Wolf Alice: 'A good album is one of the highest forms of art' - BBC News With a fourth consecutive Mercury Prize nomination, the band share their philosophy of album making.

Wolf Alice: 'A good album is one of the highest forms of art' www.bbc.co.uk/news/article...

Buddhist Hipsters — our NEW ALBUM is here!

Listen or own it on Ltd. edition vinyl & CD: orb.lnk.to/BuddhistH

"Dreamt of an escalator from the clouds - Buddhists, hipsters & Roger Eno calling me down. Now he’s on the record with friends."

Enjoy… and welcome to the future!

The US hardly seems like the most peaceful of places, under it's current president 🤷

A brilliant set by 'The Wedding Present' at @boilershop.bsky.social tonight with @earprotectreq.bsky.social David, and Anth.

👏👏👏

Save the microbes? The world’s smallest species may soon get protections. Researchers are calling for the conservation of teeny tiny organisms that normally get overlooked, and conservationists are listening.

Thanks Annie Roth! Great article on our new IUCN Microbial Conservation Specialist Group in National Geographic. www.nationalgeographic.com/science/arti...

The Energy Export Race Has a Clear Winner: China China has positioned itself as the world’s clean tech export hub while the US — especially under President Donald Trump — has pitched itself as a global gas station. That’s set up a battle for energy export supremacy. It’s solar, wind and batteries vs. oil and gas.

China is winning the energy exports race. Read more about how it's pulled far ahead of the US in today's Green Daily newsletter.

Gut microbiome comparability between DNA extraction kits The recent commentary by Manning et al highlights difficulties comparing results of gastrointestinal microbiome cohort studies.1 They demonstrate how methodological heterogeneity can substantially imp...

Gut microbiome comparability between DNA extraction kits gut.bmj.com/content/earl... < super useful - I've been waiting for this one to come out - well done Greg and @profchrislamb.bsky.social and team.

Cell-free DNA as a potential alternative to genomic DNA in genetic studies Abstract. Next-generation sequencing has greatly advanced genomics, enabling large-scale studies of population genetics and complex traits. Genomic DNA (gD

Cell-free DNA as a potential alternative to genomic DNA in genetic studies academic.oup.com/nargab/artic...

The untapped potential of short-read sequencing in biodiversity research The power of short-read DNA sequencing in biodiversity research and evolutionary genomics is rapidly growing due to advances in technology and bioinformatics. Short-read sequencing offers powerful sol...

Review | The untapped potential of short-read sequencing in biodiversity research www.cell.com/trends/genet...

Automated environmental metagenomics using Oxford nanopore sequencing - BMC Genomics Background Long-read sequencing has revolutionised metagenomics through improved metagenome assembly, taxonomic classification and functional characterisation. Automation can enhance the throughput, reproducibility, and accuracy of library preparation. However, the validation of automated library preparation protocols remains undetermined for metagenomic workflows, which are particularly sensitive to methodological perturbation. Here, we compare long-read metagenomic sequencing of environmental samples through parallel manual and automated protocols. Results Although automated library preparation led to minor reduction in read and contig lengths, taxonomic classification rate and alpha diversity was slightly higher than manual libraries, including the detection of more rare taxa. Despite this, no significant difference in microbial community structure was identified between manual and automated libraries. Conclusions Despite minor differences in sequencing and classification metrics, automated and manual library preparation resulted in comparable characterization of environmental community metagenomes. These findings demonstrate the suitability of automation for high-throughput long-read metagenomics, with broad applicability to automated long-read sequencing for improved efficiency and reproducibility.

Automated environmental metagenomics using Oxford nanopore sequencing bmcgenomics.biomedcentral.com/articles/10....