Johannes Rebelein

Last Friday was the defense of my PhD thesis entitled "Structural basis of anaerobic enzymes mediated by noncanonical metalloclusters" after 4 yrs at the @synmikro.bsky.social 🎓🎓

Big thanks to my supervisor @schullerjm.bsky.social and the whole Schuller lab for their continuous and massive support!

Very cool story about the efficient transport and incorporation of non canonical amino acid!

and Gabriella T. Heller, Simone Prinz, @georghochberg.bsky.social and Antonio Pierik.

This is outstanding work by Ana Lago Maciel and all collaborators involved: @jessicacsfla.bsky.social, Jan Zarzycki, Charlie Buchanan, Tristan Reif-Trauttmansdorff, Frederik Schmidt, Stefano Lometto, Nicole Paczia, @schullerjm.bsky.social , @dflemminghansen.bsky.social 🧪

Our latest work on the nitrogenase-like methylthio-alkane reductase, which specifically reduces reduces carbon-sulfide bonds is now out @natcatal.nature.com: doi.org/10.1038/s419.... We find for the first time large #nitrogenase metalloclusters (P- and L-cluster) outside nitrogenases.

Repurposing haemoproteins for asymmetric metal-catalysed H atom transfer - Nature Cytochrome P450 enzymes can be repurposed to catalyse asymmetric metal–hydride hydrogen atom transfer, a new-to-nature reaction.

Fantastic work by the Ward lab to repurpose cytochrome #P450 enzymes for a new-to-nature asymmetric metal-catalysed H atom transfer! Just released
@Nature
: doi.org/10.1038/s415.... A must read!🧪

Great collaboration with @lennartschada.bsky.social on an #enzyme converting the #plastic component ethylene glycol and to a feedstock chemical for microbes. Offering new ways to use plastic biotechnologically. 🧪

I am honored and excited that our research on #nitrogenases and the conversion of #CO2 to hydrocarbons is highlighted in the Yearbook 2024 of the Max Planck Society, see page 29. An English and a German version of the Yearbook are available in www.mpg.de/13631298/yea....

A must read for anybody interested in the evolution of life and how to construct phylogenies using structures. Outstanding work by @georghochberg.bsky.social and co-worker. Congrats!

A general substitution matrix for structural phylogenetics. Abstract. Sequence-based maximum likelihood (ML) phylogenetics is a widely used method for inferring evolutionary relationships, which has illuminated the

New paper from the lab from Sriram Garg in my group. We introduce a general substitution matrix for structural phylogenetics. I think this is a big deal, so read on below if you think deep history is important. academic.oup.com/mbe/advance-...

Could you please add me.

Dr. J. Rebelein(Emmy Noether Research Group Leader)

I am a microbiologist please add me www.mpi-marburg.mpg.de/859249/Johan...

Dr. J. Rebelein(Emmy Noether Research Group Leader)

I am a biochemist www.mpi-marburg.mpg.de/859249/Johan...

Two Key Ferredoxins for Nitrogen Fixation Have Different Specificities and Biophysical Properties Two ferredoxins, FdC and FdN, are collectively essential for the process of nitrogen fixation by the iron nitrogenase in R. capsulatus. We explore the biophysical factors, through spectroscopic and s....

Leveraging spectroscopic and structural approaches we obtained new insights into the role of #ferredoxins in N2 fixation. Just published in Chemistry – A European Journal: doi.org/10.1002/chem...! Great work by Holly Addison. Fantastic collaboration with Antonio Pierik on the EPR characterization.

Marburg rules. I see @rebeleinlab.bsky.social @schullerjm.bsky.social @georghochberg.bsky.social in the crowd 😘

Out now in @chemicalscience.rsc.org:

We apply 2DIR spectroscopy to study [FeFe] hydrogenase for the first time. Check it out!

pubs.rsc.org/en/content/a...

Trump gutted two landmark environmental reports — can researchers save them? Scientists working on disrupted national climate and nature assessments aren’t giving up.

The National Climate Assessment — already in the works for months — was gutted earlier this week, when President Trump’s administration dismissed hundreds of scientists working on it..

The National Nature Assessment was cancelled on his first day back in office..

🧪🦠

www.nature.com/articles/d41...

"WE'VE ARRANGED A society based on science and technology, in which nobody understands anything about science technology. And this combustible mixture of ignorance and power, sooner or later, is going to blow up in our faces. Who is running the science and technology in a democracy if the people don't know anything about it?" "Science is more than a body of knowledge, it's a way of thinking. A way of skeptically interrogating the universe with a fine understanding of human fallibility. If we are not able to ask skeptical questions, to interrogate those who tell us that something is true, to be skeptical of those in authority, then we're up for grabs for the next charlatan, political or religious, who comes ambling along."

I think a lot about what Carl Sagan said in one of his final interviews.

*** Only a few days left to apply ***

Postdoc position at the University of Marburg (Germany), funded within our ERC Advanced Grant "C-SWITCH"

Spatiotemporal regulation of cellular processes by CTP-switches in bacteria

Apply by 04 May: stellenangebote.uni-marburg.de/jobposting/2...

My dept in London at Imperial (Bioengineering) is recruiting a group leader at Professor level 🧑‍🎓 Please RT. It's a great place with world-class expertise in all sorts of biomedical & biological engineering topics like synthetic biology. It's also not in the USA. www.imperial.ac.uk/jobs/search-...

Exciting work from @frunzkelab.bsky.social and @borjana-a.bsky.social at @fz-juelich.de

Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence Bacteriophages are increasingly recognized as key players in modulating plant-microbe interactions, including their potential in the biocontrol of plant pathogenic bacteria. In this study, we investig...

New preprint from @erdrich-science.bsky.social of our collaborative project with @borjana-a.bsky.social, @fz-juelich.de and @guidogrossmann.bsky.social @hhu.de:

"Phage biocontrol reduces the burden on plant immunity through suppression of bacterial virulence"
www.biorxiv.org/content/10.1...

Heterologous synthesis of a simplified nitrogenase analog in Escherichia coli Heterologous synthesis of a nitrogenase analog (NifH/NifEN) in E. coli enables N2 reduction and incorporation of N into biomass.

Exciting work by Ribbe & Hu lab demonstrating the in vivo reduction of N2 by L-cluster containing Nif(EN)2 inside E. coli: www.science.org/doi/10.1126/... This finding suggests a smaller minimal gene set for the heterologous expression of #nitrogenase and an easier route to synthetic #N2-fixation

Great work Adrian!

Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research - Nature Biotechnology Overcoming key hurdles for the development of next-generation anaerobic cultivation methods could have major impacts on microbial community research and applications.

Enabling next-generation anaerobic cultivation through biotechnology to advance functional microbiome research www.nature.com/articles/s41... #jcampubs

This title is at best misleading. The word fertilizer probably suggests to most readers that fungi can fix molecular nitrogen (N2) to ammonia (NH3),which is not the case. So the impact of that finding is debatable...

Gab es dazu wirklich KEINE Berichterstattung? Eine Google-News Suche zu CO2-Konzentration liefert nur Ergebnisse zu einem UN-Bericht aus 2024. Keine Studie, keine News? Außer natürlich bei BIP, Inflation, Arbeitslosigkeit... 😬 Bitte gerne Links darunter posten.

Hydrogenase-driven ATP synthesis from air All cells require a continuous supply of the universal energy currency, adenosine triphosphate (ATP), to drive countless cellular reactions. The universally conserved F1Fo-ATP synthase regenerates ATP...

Energy from air? No problem! In our new preprint, we reconstitute the machinery that allows microbes to endure starvation. By using the trace amounts of hydrogen in air alone, they produce plenty of chemical energy (2 ATP) to get by. The only byproduct? Water. www.biorxiv.org/content/10.1...

Towards community-driven visual proteomics! Excited to finally share this large-scale curated & annotated dataset of 1829 high-quality #cryoET tomograms of the little green alga that just keeps giving— Chlamydomonas! 🧪🧶🧬🌾🌊🌍

Preprint📜: www.biorxiv.org/content/10.1...

A short thread🧵👇

Structure of the ATP-driven methyl-coenzyme M reductase activation complex - Nature The structure and function of the MCR activation complex from Methanococcus maripaludis were revealed, demonstrating its ATP-dependent ability to activate MCR and form methane while uncovering a uniqu...

One of the largest open questions in anaerobic metabolism solved. The best work of my lab yet, performed by the incredible hard working, talented and motivated coworkers: @fidelormz.bsky.social , @sophia-pa.bsky.social and @rnfr2d2.bsky.social. I am a proud PI today!
www.nature.com/articles/s41...