Sam Joseph

Great opportunity to work as a #PhD or #postdoc with a great PI (@mlakemeyer.bsky.social), at @uni-jena.de.

Interested in #ChemBio, #Biochemistry, #Chemistry, #Microbiology and/or pharmaceutical sciences? Check it out:

PhD: jobs.uni-jena.de/jobposting/8...
Postdoc: jobs.uni-jena.de/jobposting/c...

O-Glycans next plz

GLYCO-BUILD, an entirely enzymatic approach for the production of N-glycans and N-glycopeptides.

www.nature.com/articles/s41...

This view resonates deeply with me:

⬛ Publish fewer claims and more proof

Papers need to include fewer claims and more proof to make the scientific literature more reliable

What is driving today’s ‘claims inflation’?

1/3

Does anyone know of good conformation prediction software for PTMd peptides? There’s lots of good resources for unmodified peptides but many do not include PTMs, or extremely limited. Specifically interested in glycosylation.
#glycotime
#BioML

Ninhydrin as a covalent warhead for chemical proteomic-enabled discovery and selective engagement of reactive arginines Covalent molecules have emerged as next-generation therapeutics and as powerful tools for perturbing fundamental biological processes. Chemical proteomic methods to screen for reactive proteinaceous a...

Is arginine the new cysteine?! Check out our lab's latest in collaboration with @ianseiple.bsky.social's team where we introduce ninhydrin as a selective covalent warhead and probe targeting reactive arginines.
www.biorxiv.org/content/10.6...

Read an interesting paper on how vWF sialylation essentially increases its half-life by preventing lectin-mediated clearance from the blood stream. I wonder if there’s other examples of glycosides acting as time-gated PTMs to proteins, where “rare” glycosides = longer half-life. #glycotime

My relationship with immunology is basically represented by Stockholm syndrome.

Discovering Glycosylation-Dependent Protein Function by Thermal Proteome Profiling Protein glycosylation regulates essential cellular processes including protein folding, stability, and cell-cell interactions; however, how aberrant glycosylation impacts protein function and interact...

Congratulations former postdoc @jfhevler.bsky.social (in transit to Roche) and the team on their work using thermal proteome profiling along with glycosylation perturbagens to discover glycan-dependent protein functions at the proteome scale 👏

www.biorxiv.org/content/10.6...

Had a fantastic time at the New Horizons in Drug Discovery conference put on by @thewelchfoundation.bsky.social - also loved this slide from @dschweppe.bsky.social, sums me up pretty well

You’ve heard of #Lymedisease, but have you ever seen the bacteria that causes it? 🦠 Borrelia burgdorferi, the spiral-shaped culprit transmitted by ticks, swims with a creepy wriggling motion and uses #chemotaxis to navigate. Both fascinating... and disgusting #Microsky 🧪 #IDsky @shawlab.bsky.social

Next week, Scripps Research scientists Amy Lightner, Michael Bollong & Arnab Chatterjee will speak at #IBDCuttingEdge2025, sharing insights on regenerative medicine, clinical trials, and drug discovery in IBD.

Join us next week, Sept 19–21 | 📍 Coronado, CA
🎟️ Register now: ow.ly/xPsK50VFU8L

Ruin any book by adding "Tom Clancy's" to the title.

Tom Clancy's Protective Groups in Organic Synthesis

Gas Phase Separation of Modified Peptides for Activity-Based Protein Profiling Profiling the reactivity of the proteome with amino-acid level resolution requires the identification and quantification of reacted peptides in the presence of abundant unmodified peptides. Affinity-b...

An inspiring paper by the group of Jacob Geri in Analytical Chemistry. They present a method for residue-specific #chemoproteomics without the need for an enrichment step. To achieve this, they developed reagents that shift the ion mobility for gas phase separation. pubs.acs.org/doi/full/10....

Ultra-sensitive metaproteomics redefines the dark metaproteome, uncovering host-microbiome interactions and drug targets in intestinal diseases www.nature.com/artic...

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#proteomics #prot-paper

Probing the proteome Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. Can it deliver a new generation of drug targets?

Chemical proteomics has brought rigor to covalent drug discovery and drugs to the clinic. A feature in Nature Biotechnology explores whether it can deliver a new generation of drug targets. 🧪

PNGaseL from Flavobacterium akiainvivens targets a diverse range of N-glycan structures PNGases are used by a wide range of organisms to remove N-glycan structures from proteins for use as either nutrients or in glycoprotein processing. PNGaseF is the most well-characterised enzyme of th...

Very pleased to present the next pre-print from our lab #glycotime We showcase PNGaseL from Flavobacterium akiainvivens that we hope will be a useful tool for the glyco community 🍬🧪👩‍🔬 Special shout out to @cassiebakshani.bsky.social and Paulina (Ludger)
www.biorxiv.org/content/10.1...

Biblically accurate nitrogen

A "cross-decoupling" or "retro-Suzuki" sequence

Fans of Guenther Schlonk will enjoy the "cross-decoupling" easter egg:

jabde.com/wp-content/u...

Checking USCSB quarterly is one of my favorite hobbies

Are you really a synthetic chemist if you aren't watching USCSB videos?

Senator Durbin noted that some (I believe Republican Senators) had indicated the US had been doing more than its share of biomedical research and it was time for other countries take a turn.

I felt I had to interrupt to respond...

Yikes

Exciting news: We have released #FragPipe 23, and it's one of our biggest updates ever. Windows installer, support for TMT on Astral and timsTOF, TMT35, PTM site reports for DIA, improved Astral data handling in #MSFragger, improved diaTracer for diaPASEF data, better Skyline integration, and more!

Excited to announce that I passed my 2nd year qualifying exam! This is where the fun begins…

If u ever had to depict proteins as schematics in papers, u know the dilemma: how to create a pretty “blob”?
If structure ends up big & central, u’ll render it with PyMol et al, but for a truly schematic depiction u want a biorender-style icon, but for YOUR protein.
Think I’ve finally found a way.

The 15th International ABPP Meeting will be happening in Leiden in 2027. While it is still a long way to go, if you enjoyed this year's edition or were sad that you missed out, make sure to already follow @abpp2027.bsky.social to receive all the information in the future!

Self-assembled PROTACs enable glycoproteins degradation in the living cells We report here a two-component proteolysis targeting chimeras (PROTACs) strategy selectively targeting O-GalNAcylated and O-GlcNAcylated proteins for proteasomal degradation, which leads to severe tox...

Great to see the new concept of protein PTMs functionalization published in @chemicalscience.rsc.org! The GlyTACs enable selective degradation of glycoproteins in living cells. Congratulation to Haoyu and Liu. Have a look on the full story here: pubs.rsc.org/en/Content/A... #chembio

is.... that a capybara for scale

Had a fantastic time at #ABPP2025, my first ABPP meeting. Saw outstanding talks, shared what I’ve been working on, and was proud to see my undergrad, Abbey Pitts, win best poster award. Keep an eye out for her, she’ll be applying to PhD programs this Fall. Can’t wait for ABPP 2027!