Conor Sugden

LPS increases Actb mRNA in PeMPs in vivo and enhances its localized translation in vitro. New synthesis of β-actin in microglial peripheral structures was assessed with a 2-min puromycin pulse in PBS- and LPS-treated cells followed by proximity ligation assay (PLA) with antibodies against puromycin and β-actin. As a negative control, cells were preincubated with the protein synthesis inhibitor anisomycin. Micrographs show the PLA signal in microglia labeled with phalloidin. Arrowheads indicate PeMPs depicted in insets (4 rightmost panels). Scale bars 20 µm (insets 10 µm)

Local #mRNAtranslation in polarized cells is well studied in neurons but what about #microglia? This study shows that #inflammation enhances microglial #RNA localization & translation via RNA-binding protein IMP1/ZBP1, regulating morphology, motility & phagocytosis @plosbiology.org 🧪 plos.io/486ZLMS

The ECM is no longer in the back seat when it comes to development. Check out our special issue "Dynamics of the extracellular matrix in development, cell physiology and disease" with 8 selected articles to learn more: A 🧵 www.sciencedirect.com/special-issu...

The IMAG'IC team is back to offer you the "Introduction to the Use of #ImageJ" training course.
October 24, 2025: Session in French
October 29, 2025: Session in English
institutcochin.fr/formations/f...

@institutcochin.bsky.social
@upcite.bsky.social
@inserm.fr

We're hiring new faculty! Looking for talented scientists that are interested in pushing the boundaries of Cell Biology. Please spread the word!

🔴✨ Exciting opportunity : we're hiring a PhD student! ✨🔴

👉 Don't hesitate to apply or spread the word!

⏰ Deadline 30th November 2025

ℹ️ More information and application : www.crg.eu/en/content/t...

@crg.eu @prbb.org

[Commentary] Distal enhancers loop to proximal enhancers, not to promoters by the one and only Kevin Struhl in #genesky @natrevmcb.nature.com

www.nature.com/articles/s41...

Super-Resolution Imaging Reveals Stretch-Induced Architectural Rearrangement of Desmoplakin in Desmosomes https://www.biorxiv.org/content/10.1101/2025.08.26.672431v1

Our protocol for gene editing is online and all plasmids are on @addgene.bsky.social! CRISPR away everybody and get in touch if you need anything 🤩

Lentiviral vectors have stably integrated themselves as a favorite in the viral vector toolbox. In our recently refreshed lentiviral guide, we cover the basics of lentiviral vectors including generations, production, common uses, and safety. Check it out!
https://twp.ai/4ipGPa

Wonderful to be at the 15th International Podocyte Conference in Hamburg! Exciting science & fabulous networking ❤️

Huge 🙏 to @tobias-b-huber.bsky.social & @isgd.bsky.social teams 🙏

Newest version of Cellpose can spot cell boundaries even in cloudy conditions Picking out individual cells in distorted microscopy images is now as easy as clicking a button. A new version of Cellpose – the popular tool that maps the boundaries of diverse cells in microscopy

🖱️ Picking out individual cells in distorted microscopy images is now as easy as clicking a button, thanks to Cellpose3: www.janelia.org/news/newest-...

Amazing postdoctoral position at Pernaute’s lab

Fully funded for 3 year by European Research Council (#ERC) grant EARLYFATE #ERCCoG

At the #CABD a research center recognized with a national excellence award ‘María de Maeztu’ located in Seville, Spain

Share or apply

#JobOffer
#Postdoctoraljob

For clear thinking, cultivate passionate indifference. Buddhists call it non-attachment. We all have hopes, desires and ambitions. Non-attachment means acknowledging them, accepting them, and then not inserting them into a process that has nothing to do with you. podcasts.apple.com/us/podcast/n...

On April 19, 1898, Camillo Golgi described what we now know as the #Golgi apparatus - a structure so pretty and unusual it took over fifty years for the scientific community to agree it wasn’t just an artefact.
April 19 is #GolgiDay! Mark your calendars to honour the best organelle.

Practical considerations for data exploration in quantitative cell biology Summary: We present practical guidelines for data exploration in quantitative cell biology that are designed to help yield reliable conclusions and promote a collaborative, transparent approach to dat...

Here we share our opinions on "Practical considerations for data exploration in quantitative cell biology"
journals.biologists.com/jcs/article/...

Joanna, Hanna and I worked quite a bit on this piece and I hope you find it useful
Many thanks to @jcellsci.bsky.social for publishing it

Contrary to popular belief, what is important in science is as much its spirit as its product: it is as much the openmindedness, the primacy of criticism, the submission to the unforeseen, however upsetting, as the result, however new that may be. – Francois Jacob
www.nature.com/articles/s41...

Join the biocontrol seminar tomorrow to hear about the fun things we are doing with controlling cells!

This will never stop blowing my mind.

Looking for #MaterialStiffness of #BasementMembrane across tissues?
Excellent literature resource here😆👇

The basement membrane as a structured surface – role in vascular health and disease

@claireleclech.bsky.social @jcellsci.bsky.social 2020
journals.biologists.com/jcs/article/...

The pathways of secretory cargo export at the endoplasmic reticulum Nature Communications - The author presents an overview of the complex challenge of protein export from the Endoplasmic Reticulum, and discusses how cells may deploy novel pathways utilizing...

A terrific perspective from Vivek Malhotra @naturecomms.bsky.social on the fundamental importance of pathways and mechanisms by which cells secrete proteins, ensuring their delivery in the correct quantities and at the appropriate times.
Interesting section on unanswered questions.

rdcu.be/ecb2B

Rhobo6👹
An omnipotent cell-impermeable dye labels diol/glycan of #ExtraCellularMatrix
✅collagen/fibron, BM, proteoglycan

Retro-orbital injection to label the ECM throughout WHOLE mouse body▶️intravital 2P

Also work on🪰🪱🐟🌱

@naturemethods.bsky.social 2025
www.nature.com/articles/s41...

🔬✨ SCIENCE THAT GLOWS💡🔴

Conor Sugden, Shota Kaseda, Sherwood Lab & UoM’s Genome Editing Unit made basement membranes glow red with mScarlet. This helps scientists see damage & repair in real-time—leading to better treatments! 🏥💙

@conorjsugden.bsky.social
@rachellennon.bsky.social

Come together, right now The chaos, conflicting information, firings, and hurtful rhetoric of the Trump administration’s approach to science over the past month are causing anxiety, grief, and concern for the scientific commu...

🔔🧪Let’s support our colleagues:

👉“The upcoming weeks will challenge the US #scientific #community, emphasizing the need for #unity and #support among its members. Upholding principles of #independence, #peer evaluation, and #inclusion is vital for future #success.”

www.science.org/doi/10.1126/...

Exiting the microscopy room

#CellMigration happens all the time in our bodies. This may (or not) rely on adhesion molecules

#Cancer cells switch strategies during metastasis to invade other tissues

Naomi Wee (naomiwee.com) did this neat movie to explain it:
www.youtube.com/watch?v=D9aD...
#SciSky 🧪🔬 @cellcommlab.bsky.social

I put together a small guide on how to get started with Fiji. Please distribute to anyone who might benefit form this! zenodo.org/records/1477... #imageanalysis #fiji #imagej #training

Light-activated binding of a protein to tubular lysosomes:

🚨 Brand new website for Matrix Biology Italy #MBIta (formerly know as Italian Society for the Study of Connective Tissue)
www.matrixbiology.it

@valerioizzi.bsky.social @themeshwork.bsky.social @nabalabuic.bsky.social @tomvanagtmael.bsky.social @amrohussien.bsky.social @tcox.bsky.social

Failure is always in the background of any creative project; when the experiments are not working or the results don’t seem to make sense. But for discovery, you can’t deny yourself the permission to fail, and we should better teach failure in all its glory.

Trypsin treatment improves Cellpose segmentation of brightfield images for adherent cells

Accessible and accurate cytometry analysis using fluorescence microscopes by Bo Huang and team:
www.biorxiv.org/content/10.1...