Carsten Sachse

We are looking for a team leader to join the cryo-EM facility work at the new Ernst Ruska-Centre 2.0 at the Forschungszentrum Jülich. We have a series of microscopes used by (@irene-vercellino.bsky.social, Schröder, @sachsegroup.bsky.social) and external users.
www.fz-juelich.de/de/karriere/...

In ATG5 KD conditions, p62 wraps around lipid droplets including Ca-rich layer revealed by EDX spectroscopy. Early visual of autophagy - Thanks to Sabrina, Lisa, Alex, Siavash and everyone involved including our collaborators Natalia Kononenko, Rafal Dunin-Borkowski and @degradomics.bsky.social

Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo - Nature Communications The selective autophagy receptor p62/SQSTM1 is organized in a structured double helical filament in vitro, the cryo-electron tomographic images in ATG5 knockdown conditions reveal Ca-rich layers of p6...

Really happy to see the work (doi.org/10.1038/s414...) published. Here, we show the full-length helical cryo-EM structure of p62/SQSTM1 and first cryo-ET visual of p62 positive structures inside the cell.

Grateful to have received the Wilhelm und Else Heraeus-Stiftung Research Fellowship, to support my group’s work at the Ernst Ruska-Centre (ER-C) of Forschungszentrum Jülich #cryoem

Finally in peer-reviewed form: Mapping the ultrastructural topology of the corynebacterial cell surface. @bupbuse.bsky.social @vikramalva.bsky.social

Please also see concurrent beautiful work from @adriasogues.bsky.social and Han Remaut.

Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures | PNAS The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been num...

After years parked at a Polara, I’m excited to share that my PhD work is finally out! We figured out why imaging at liquid helium temperatures wasn’t working—and how to fix it.
🔗 www.pnas.org/doi/10.1073/...

CryoVIA: An image analysis toolkit for the quantification of membrane structures from cryo-EM micrographs Imaging of lipid structures and associated protein complexes using cryoelectron microscopy (cryo-EM) is a common visualization and structure determina…

Philipp's manuscript on our CryoVIA toolkit www.sciencedirect.com/science/arti... is out! For those interested in studying protein lipid interactions and high-throughput analysis of membrane structures (sizes, shapes, curvature etc.) - this could be your tool (github.com/philipp-scho...).

CryoVIA - An image analysis toolkit for the quantification of membrane structures from cryo-EM micrographs Imaging of lipid structures and associated protein complexes using electron cryo-microscopy (cryo-EM) is a common visualization and structure determination technique. The quantitative analysis of the ...

Our first post on blue: grad student Philipp's software: for those interested in looking at membrane reconstitution by cryo-EM. Automated analysis of lipid fine structure and membrane shape classifications: biorxiv.org/cgi/content/...

Excited to share that I have been elected as member of the "Junge Kolleg" of the Nordrhein-Westfalian Academy of Sciences and Arts. I am honoured to have this opportunity to serve the community! Here's the link to the announcement, unfortunately only in German www.awk.nrw/news/nachwuc...