Doug Dyer

Love a story where the environment wins the argument every single time

This is also an elegant dissection of how the microenvironment cues, differentiation pathways, and inflammatory signals converge to shape the glycocalyx

Thanks for sharing @douglaspdyer.bsky.social

Airway macrophage specific expression of a dense glycocalyx and its remodelling following viral infection The glycocalyx is best studied for its regulation of immune cell trafficking from the vasculature into inflamed tissues. The glycocalyx is composed of a range of glycans and glyco-proteins that form a...

Delighted to share our new pre-print from PhD student Ziyun Zhang joint with @tracyh9494.bsky.social demonstrating that lung alveolar macrophages have a dense glycocalyx and interstitial macrophages do not, @mcrcellmatrix.bsky.social @lydiabeckeriii.bsky.social www.biorxiv.org/content/10.1...

Thanks goes to Critical technical and conceptual contributions from @iashiamulholland.bsky.social 🧪 #glycotime

This the lung micro environment regulates the macrophage glycocalyx via local cytokines at rest and following infection, a process likely crucial to viral infection

To fully determine the effect of the lung environment on the glycocalyx we transferred macrophages with low glycocalyx into the airways space and saw that this drove an increase in the transferred cells glycocalyx

Using an in vivo flu infection model we showed that the effect of type 1 cytokines is overridden by the lung environment, with alveolar and interstitial macrophages increasing their glycocalyx at 7 and 14 days post infection

To verify the effect of type 1 cytokines on the macrophage glycocalyx we stimulated a macrophage cell line with the viral mimic poly I:C resulting in glycocalyx shed

She again probed this at the transcriptional level with very differential effects on genes that regulate the cell surfaces glycocalyx

She then further polarised her macrophages with type 1 and 2 cytokines with contrasting effects on glycocalyx content

Next she explored glycocalyx regulation by proving the effect of macrophages derived with M-CSF vs GM-CSF

Macrophage

Zi first used imaging and flow cytometry to show that alveolar macrophages in the airways have a strong WGA detectable glycocalyx

Airway macrophage specific expression of a dense glycocalyx and its remodelling following viral infection The glycocalyx is best studied for its regulation of immune cell trafficking from the vasculature into inflamed tissues. The glycocalyx is composed of a range of glycans and glyco-proteins that form a...

Delighted to share our new pre-print from PhD student Ziyun Zhang joint with @tracyh9494.bsky.social demonstrating that lung alveolar macrophages have a dense glycocalyx and interstitial macrophages do not, @mcrcellmatrix.bsky.social @lydiabeckeriii.bsky.social www.biorxiv.org/content/10.1...

Tissue resident colonic macrophages persist through acute inflammation and adapt to aid tissue repair Macrophages are crucial for the maintenance of intestinal homeostasis, are considered key proinflammatory effector cells during intestinal inflammatio…

Thrilled for our wonderful postdoc @lizihegarty.bsky.social whose #PhD work on colonic #macrophages and #colitis with @bainlab.bsky.social @edinuni-irr.bsky.social has been accepted for publication in @mucosalimmunol.bsky.social 🔬

www.sciencedirect.com/science/arti...

- A postdoc to work on cancer extravasation (4-year project).
- A postdoc help in completing Filopodia-related lab projects (2-year).
- Multiple Marie Curie postdoc positions that will open in the fall.
- We will open a 5-year position to cover my teaching (70% research and 30% teaching).

Less than 3 weeks until our final report launch event
9th Dec. (London) 10am

We will be welcoming a fantastic panel:
🌟Jo O'Leary (UKRI)
🌟Sara Mole (UCL)
🌟John Tregoning (Imperial)
🌟Katie Perry (Daphne Trust)
🌟Jennie Evans (BSI)
🌟Ilaria Meazzini (RSC)

Register here: www.carersinstemm.co.uk

Big day for foundational glycoRNA research, with two preprints published in Nature EMM. Both urge greater analytical rigour in the field.

1. RNA-associated glycoconjugates highlight potential
ambiguities in glycoRNA analysis

2. Proteins are a source of glycans found in preparations of glycoRNA

Join us in Sheffield! We are keen to support early career researchers in applying for independent research fellowships (IRFs) to join our friendly and collaborative environment, in areas related to developmental biology, stem cells and regenerative biology, and neuroscience.

Another bump for the postdoc and PhD positions still open at the center and postdoc specifically within my group (same link to apply). We’re looking for wet/dry lab, and I’m specifically after people who do magic with computers!

B4GALT1 and Wntless collaborate to block LRP5/6 translocation from #Golgi to cell surface, say Xinyang Li, Yue Hua, Xiaoqing Gan and colleagues (Fudan University): rupress.org/jcb/article/...

#Biochemistry #Trafficking #CellSignaling #Wnt

Don’t miss this work on CCR2 monocyte recruitment from @douglaspdyer.bsky.social and colleagues - online now! www.mucosalimmunology.org/article/S193...

CCR2-driven monocyte recruitment is protective against radiotherapy-induced intestinal toxicity
www.mucosalimmunology.org/article/S193...

Well done @nabinapun.bsky.social getting your Radnet @cruk-mi.bsky.social @lydiabeckeriii.bsky.social @mcrcnews.bsky.social @mcrcellmatrix.bsky.social PhD work out

If you believe either that Franklin discovered the double helix, and / or Watson and Crick stole her data, ask yourself how you know this. Then take a read of this article.

Immunology 101: Monoclonal antibodies What monoclonals are and how we use them therapeutically.

It’s a new immunology 101 to brighten your weekend cos your weekend can’t get started until you have marvelled over monoclonal antibodies. Its a reach I know but still hope you find time to read and enjoy 🧪

Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during psoriasis-like skin inflammation Leukocytes accumulate in the skin during psoriatic-like inflammation upon cleavage of surface heparan sulfate.

New findings in #ScienceSignaling show that cells called leukocytes have a surface “coating” that can be modified during inflammatory conditions, such as #psoriasis, revealing how immune cells are recruited to sites of inflammation. https://scim.ag/4osBp5U

Thanks so much for inviting me, it was a great day and a particularly impressive group of students!!

Day 2 of the Matrix Biology PhD course and we’re in the new Fælleshuset building starting our group work brain storming. Thanks to @douglaspdyer.bsky.social and @tranejoan.bsky.social for laying the foundations of ECM biology in yesterday’s lectures!

@mcrcellmatrix.bsky.social

Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during psoriasis-like skin inflammation Leukocytes accumulate in the skin during psoriatic-like inflammation upon cleavage of surface heparan sulfate.

Leukocytes accumulate in the skin during psoriatic-like inflammation upon cleavage of surface heparan sulfate
www.science.org/doi/10.1126/... @megtriesscience.bsky.social @douglaspdyer.bsky.social

The image shows H&E-stained inflamed mouse ear skin, showing accumulated leukocytes.

Don’t miss this week’s issue of #ScienceSignaling!

New discoveries about an enigmatic, cancer-linked mutation could redefine understanding of the kinome, a study reveals how immune cells are recruited to inflammatory sites in psoriasis, and more. https://scim.ag/3LkFGtC