Hocek lab

Nucleic acid chemistry This Collection aims to offer insight and inspiration for nucleic acid chemistry, focusing on the structure, function, generation, modification, ...

Our collection on “Nucleic acid chemistry” is now complete, edited by guest editors Andrea Rentmeister @rentmeisterlab.bsky.social and Michal Hocek @hoceklab.bsky.social. Congratulations!
www.nature.com/collections/...

Picomolar inhibitors of CD73 developed in great collaboration with Jan Konvalinka group. Check out our latest paper in ACS Pharmacol. Transl. Res.: doi.org/10.1021/acsp... @iocbprague.bsky.social

Hydrophobic nucleoside phosphoramidites for solid-phase synthesis of hypermodified oligonucleotides. Our new paper published in RSC Advances doi.org/10.1039/D5RA... @iocbprague.bsky.social @akademievedcr.bsky.social @sciencecharles.bsky.social

Our new paper on Enzymatic synthesis of base-modified RNA and influence of the modifications on stability, translation, and CRISPR-Cas9 cleavage - published in ACS Chem. Biol.: doi.org/10.1021/acsc.... @iocbprague.bsky.social @sciencecharles.bsky.social @akademievedcr.bsky.social

Our newest paper Versatile Enzymatic Approach for Truncation, Labeling, and Mutation of Nucleobase-Modified Aptamers Demonstrated on Indole-Modified β-Conglutin Aptamer published in ChemBioChem: doi.org/10.1002/cbic... ; @iocbprague.bsky.social

Well, we did the chemistry and biochemistry part - the chromatin changes in neuron differentiation was studied by @cardosolab.bsky.social

New paper in great collaboration with @cardosolab.bsky.social on Super-Resolution Compatible DNA Labeling Technique Reveals Chromatin Mobility and Organization Changes During Differentiation published in Advanced Science: doi.org/10.1002/advs... @iocbprague.bsky.social

Kristýna Blažková se společně s kolegy z vědeckých skupin Jana Konvalinky a @hoceklab.bsky.social zaměřila na enzym #CD73, který využívají nádorové buňky k útlumu imunitního systému tak, aby nebyl schopný bránit rakovinnému bujení. 3/7

Polymerase synthesis of base-modified DNA scaled-up to milligram quantities suitable for NMR structure studies. Check our newest work published in ChemistryEurope doi.org/10.1002/ceur... @iocbprague.bsky.social

Congratulations to the winners of the #ChemEurJ & #ChemBioChem Poster Prizes at the recent #SCNAC2025 - and many thanks to the jury for their hard job making a choice among all the stellar posters!

Kudos to Lessandro De Paepe, Tania Sanchez Quirante & Qiyang Zhang ! 👏

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Natalia Kuprikova has successfully defended (at the Charles University) her PhD thesis entitled Enzymatic synthesis of hypermodified DNA bearing anionic or cationic functional groups. PhD No. 48 from the Hocek group. Congratulations!

Our newest paper on enzymatic incorporation of 2-substituted adenine nucleotides into RNA and their use for RNA labeling has been published in ChemBioChem: doi.org/10.1002/cbic... @iocbprague.bsky.social

Polymerase Synthesis of Hypermodified DNA Displaying a Combination of Thiol, Hydroxyl, Carboxylate, and Imidazole Functional Groups in the Major Groove A series of 2’-deoxyribonucleoside 5’-O-triphosphates bearing functional groups of amino acid side chains in enzyme active sites (OH, SH, COOH, and imidazole) were synthesized and used for enzymatic ...

Our newest paper on enzymatic synthesis of hypermodified DNA bearing functional groups mimicking amino acid side chains in enzyme active sites just published in ChemEurJ. doi.org/10.1002/chem... @iocbprague.bsky.social @akademievedcr.bsky.social

Ugnė Šinkevičiūtė has successfully defended the Charles University her PhD thesis entitled: New deazapurine nucleobases, nucleosides and nucleotides as potential cytostatics or CD73 inhibitors. PhD No. 47 from the Hocek group. Congratulations !

Our newest paper on enzymatic synthesis of hypermodified zwitterionic DNA bearing 4 different cationic groups has just been published in NAR. doi.org/10.1093/nar/...

Filip Gracias has successfully defended at UCT Prague his PhD thesis entitled: Synthesis of epigenetic pyrimidine nucleotides and their non-natural analogues for regulation of transcription. PhD No. 46 from the Hocek group. Congratulations Dr. Gracias! @iocbprague.bsky.social

Deadline for oral presentation abstracts for the XIXth Symposium on Chemistry of Nucleic Acid Components, Cesky Krumlov, June 8-13,2025 (www.scnac.com) is close (February 20, 2025) - if interested, please apply!

me too

🧑‍🔬 PhD projects in 2025

See more about the project of @hoceklab.bsky.social at ► www.uochb.cz/en/open-posi... ⤵️

#phd #chemistry #chemie #phdatiocbprague

Our new paper (collaboration with P. Rezacova group) on the use of environment-sensitive fluorophore-linked nucleotide in DNA probes for identification of effectors of TFs and studying conformational changes of protein-DNA complexes has been published in RSC Chem. Biol.: doi.org/10.1039/D4CB...

🧑‍🔬 PhD projects in 2025

See the project of @hoceklab.bsky.social at ► uochb.cz/en/open-positions/320 ⤵️

#phd

Can I join as well ?

#research

Enzymatic synthesis of reactive RNA probes containing squaramate-linked cytidine or adenosine for bioconjugations and cross-linking with lysine-containing peptides and proteins (Hocek): nature.com/articles/s42...

@iocbprague.bsky.social #charlesuniversity @czechacademy.bsky.social