Sahar

I love when I read preprint's title, think "oh this sounds a lot like what lab X works on", and i open the author list and its the same lab/PI. its a small community :)

An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis - Nature Cell Biology Xia, Zhang, Peng, Chen et al. find that Leydig cells and macrophages show bidirectional mitochondrial transfer through extracellular vesicles, ensuring Leydig cells have functional mitochondria. The e...

☕Xia, Zhang, Peng, Chen et al. find that #Leydig cells and macrophages show bidirectional mitochondrial transfer through #ExtracellularVesicles, ensuring Leydig cells have functional #mitochondria. The exchange is needed for testosterone production.
bit.ly/40US4Vy

Design of the protein FRET ladder

Fancy a fresh preprint for Friday? When we were first getting involved with single molecule FRET, there weren't any standard protein molecules that suited our applications to help us develop our pipeline. So we built some! A universal protein ladder for FRET. 🧵 1/

a lot of interorganelle contact research coming out this year and im loving it 🤩

Hoi1 targets BLTP2 to ER–PM contact sites to regulate #lipid homeostasis. New study from Samantha K. Dziurdzik, Vaishnavi Sridhar, Elizabeth Conibear @lizconibear.bsky.social and colleagues (University of British Columbia): rupress.org/jcb/article/...

#Genetics #MembraneContactSites #ER_literature

Data Visualization A Practical Introduction

Here’s a full draft of the upcoming second edition of my “Data Visualization: A Practical Introduction”: socviz.co

Figure 1. Cryo-EM of abundant protein complexes in native membranes.

Figure 2. Cryo-EM of membrane proteins in vesicles.

Figure 3. 3D reconstruction of V-ATPase in native synaptic vesicle membranes.

Figure 4. Generation of membrane vesicles for structure determination of proteins in their native lipid bilayer.

I've written a review on what I think is an extremely exciting direction in cryo-EM:

Cryo-EM of endogenous membrane proteins in their native lipid bilayer

Open access in Quarterly Reviews of Biophysics:
doi.org/10.1017/S003...

me and my go train analysis sessions 🤭

had a lot of lab failures today, felt so defeated but I kept myself firm to finish the rest of my experiments and my day ended with some really interesting preliminary data :) grad school words to live by: "just keep moving forward"

Why can’t people (especially scientists) see that AI generated figures and diagrams shout out that they were AI generated and look awful? You’ve spend many months designing and performing experiments only to cover the resulting masterpiece in clear plastic like a cheap sofa.

Armoring CAR T cells with the ability to secrete VEGF-neutralizing single-chain variable fragments enhances potency and control of tumors in vivo
www.science.org/doi/10.1126/...

The molecular basis of force selectivity by PIEZO2 Nature - PIEZO2 is intrinsically more rigid than PIEZO1, and disparate mechanical stimuli paradoxically evoke opposite conformational and gating responses in each channel.

New paper from the lab led by @ericmulhall.bsky.social addresses how PIEZO1 and PIEZO2 are tuned to different types mechanical forces. From nanometer-scale super-resolution microscopy to in vivo experiments, links single-molecule observations to physiological function.
www.nature.com/articles/s41...

Contact with dead cells or IgG-coated surfaces during efferocytosis triggers a Rubicon–VPS34-phospholipase induced integrin barrier that excludes Notch from contact sitem hampering anticancer immunity
www.nature.com/articles/s41...
@natimmunol.nature.com @stjuderesearch.bsky.social

STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria - The EMBO Journal STIM1 is a transmembrane protein localized in the endoplasmic reticulum (ER), where it acts as a calcium ion sensor, activating store-operated Ca2+ entry upon ER Ca2+ depletion. Via cellular calcium i...

link.springer.com/article/10.1...

Distinct impact of PI(4)P flux on PI(4,5)P2 steady states and oscillations | PNAS Plasma membrane (PM) phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] regulates indispensable processes such as exocytosis, endocytosis, and actin...

A fascinating look at the frankly bonkers, still-mysterious relationship between master membrane regulator PIP2 and its precursor. Another classic from Min Wu's lab!

single molecule and intravital?? my mind is blown

Mitochondrial fission during mitophagy requires both inner and outer mitofissins - EMBO Reports Mitophagy maintains mitochondrial homeostasis through the selective degradation of damaged or excess mitochondria. Recently, we identified mitofissin/Atg44, a mitochondrial intermembrane space-residen...

❄️☕👩🏻‍💻

link.springer.com/article/10.1...

My quote of the day

Science is the search for truth, that is the effort to understand the world: it involves the rejection of bias, of dogma, of revelation, but not the rejection of morality.

Linus Pauling

Omg the graphical abstract is awesome

can engineered secreted proteins drive cells into diverse states? important work showing non-native signaling can do just that!

reminds me of recent work from Marc Exposit, Mo Abedi et al in the Baker lab www.biorxiv.org/content/10.1...

😯

🙏 This study was powered by fantastic collaborations — especially with @abelljonny.bsky.social & @joechambers.bsky.social with contribution form @felixmendu.bsky.social @lasergroup.bsky.social @franbottanelli.bsky.social 🔬🧠

www.nature.com/articles/s41...

really creative experiments, amazing read 🤩

DNA nanodevices detect an acidic nanolayer on the lysosomal surface - Nature Cell Biology Tan and colleagues develop DNA nanodevices to detect the pH of the lysosomal outer surface, observing an acidic layer generated by TMEM175 that regulates lysosome positioning in response to changes in...

☕ @zyt0329.bsky.social et al develop DNA nanodevices to detect the pH of the lysosomal outer surface, observing an acidic layer generated by TMEM175 that regulates lysosome positioning in response to changes in juxta-lysosomal pH.
👉https://rdcu.be/e3TPL
bit.ly/4auh4aD

An mTurquoise2-Based Glucose Biosensor Glucose is an important substrate for organisms to acquire energy needed for cellular growth. Despite the importance of this metabolite, single-cell information at a fast time-scale about the dynamics...

Great to see this officially out: An mTurquoise2-Based Glucose Biosensor - works in yeast & mammalian cells and is compatible with FLIM: pubs.acs.org/doi/10.1021/...

Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis"

www.biorxiv.org/content/10.6...

Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo

p53 increases phospholipid headgroup scavenging in senescence - Nature Cell Biology Yashinskie, Zhu and colleagues show that p53 activation triggers increased synthesis and accumulation of phospholipids, with enhanced activation of autophagy and lysosomal catabolism programmes and in...

☕Yashinskie, Zhu et al show that #p53 activation triggers increased synthesis and accumulation of phospholipids, with enhanced activation of #autophagy and lysosomal catabolism programmes and increased reliance on lipid headgroup recycling. @lydiafinley.bsky.social
bit.ly/4asbwO3

having the privilege to be surrounded by cell biologists and research is still unreal to me 🥹

so lucky to have lab meetings where we go over diverse projects, it gets you thinking about new cell biology questions. downside: its nearly midnight and im still thinking about acetyltransferases 🧍‍♂️

got trained on abberior's stedycon!! so happy