ProImmune

This #FluorescenceFriday we used our recombinant target binding reagent Ankyron® clone CE30111 (green) to detect Perlecan in the extracellular matrix deposited by HUVECs, with nuclei counterstained using DAPI (blue). Image captured using our brilliant @discoverecho.bsky.social #microscope.

This #FluorescenceFriday we’re exploring microtubules using Ankyron® clone AP40945, which binds TBCA (red), with DAPI-stained nuclei (blue). Shown in HeLa cells. Image captured with our lovely @discoverecho.bsky.social #microscope. Learn more: proimmune.com/ankyron

If #FlowcytometryFriday is more your thing, then Ankyron™ AC40302 works well in this application, shown in HeLa (red histogram, with negative control of secondary fluorophore only in grey).

Fascinating insights this #FluorescenceFriday, into complement delay-accelerating factor (DAF, CD55), identified by our Ankyron™ AC40301 (red) in HeLa cells (nuclei, blue) on @discoverecho.bsky.social #microscope.
Find out more about our #animal-free Ankyrons™ at www.proimmune.com/ankyron

Exquisite filamentous patterns for #FluorescenceFriday thanks to Ankyron™ AU41488 specific to CD29 cell surface receptor β1 integrin (red) in a dendritic cell, with nucleus counterstained with DAPI (blue), on our lovely @discoverecho.bsky.social Echo Revolve #microscope.

#immunology #animalfree

#MicroscopyMonday! IHC with an insulin-specific #animalfree Ankyron AB40258 (brown) clearly identifying β-cells in islets of Langerhans of human pancreas (nuclei in blue) using #Leica autostainer and @discoverecho.bsky.social #microscope in colour camera upright configuration. proimmune.com\ankyron

This #FluorescenceFriday we use an Ankyron™ to specifically label the Golgi apparatus marker RCAS1 (red), counterstaining actin cytoskeleton (green) and nucleus (blue) on @discoverecho.bsky.social microscope. Look out for our other organelle-specific #animalfree Ankyrons™ at proimmune.com\ankyron

We were happy to see that this Ankyron also works well in #FlowCytometry, with HUVEC stained with V5-tagged Ankyron and anti-V5 APC (red). Negative control, anti-V5 APC only (grey).

Shining a light on Endoglin (aka CD105) for #FluorescenceFriday, shown in red using #animalfree Ankyron™ clone AY40419 (nuclei blue), highly expressed in vascular endothelial cells like these HUVEC with crucial roles in angiogenesis & TGFβ signaling. On our @discoverecho.bsky.social #microscope

This dendritic cell is ready for #FluorescenceFriday! Here, we used our interferon-α8-specific #animalfree Ankyron™ ANK01178_5_AW40550 (red) and nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
Find out more:
www.proimmune.com/ankyron
#cytokine
@afruk.bsky.social

#FluorescenceFriday insight into Lupus La protein, highly distinctive cytoplasmic staining (red) revealed by our #animalfree Ankyron™ - nuclei counterstained with DAPI (blue) on @discoverecho.bsky.social #microscope
#bioscience #immunology #animalfreeresearch #cytoplasm
@afruk.bsky.social

Delighted that we contributed to exciting work from Zuben Sauna’s lab (FDA) using Ankyrons™ specific to 3 #COVID19 spike protein variants, which neutralized all 3 in a pseudovirus-based neutralization assay.

doi.org/10.1208/s122...
#BLI #immunology #animalfree

It's #FluorescenceFriday! Here's a lovely clear image using #animalfree Ankyron™ specific to cell cycle protein Cyclin A1 (red), with F-actin counterstained by phalloidin (green) on @discoverecho.bsky.social #microscope Find out more www.proimmune.com/ankyron #immunology #3Rs
@afruk.bsky.social

Our very own Emilee Knowlton, Ph.D., Senior Immunology Sales Specialist, will be speaking at this event about "An Integrated Approach to Managing Immunogenicity Risk and Optimum Protein Design".

We're sponsoring #festofbiologics where science meets strategy!

Join us on April 23-24 in San Diego. From cutting-edge research to advancements in AI-driven drug discovery, this is where breakthroughs happen.

And here's the same #animalfree Ankyron™ specific to β-actin (red), lighting up the cytoskeleton that we highlighted for #FluorescenceFriday last week:

Is #WesternBlotWednesday a thing?! Why not celebrate those perfect western blots? Here's our #animalfree Ankyron™ specifically binding human β-actin (37.3 kDa), both recombinant protein and in cell line lysates. Perfect loading control for westerns!
#3Rs #westernblot
@afruk.bsky.social

Fab fine filaments of the cytoskeleton for #FluorescenceFriday lit up by our #animalfree Ankyron™ to β-actin (red), with nuclei counterstained with DAPI (blue) on our Echo Revolve #microscope @discoverecho.bsky.social An alternative counterstain to phalloidin! #immunology #3Rs @afruk.bsky.social

Spectacular cell structure #FluorescenceFriday, with an #animalfree Ankyron™ against Calreticulin to specifically label the endoplasmic reticulum (red) in HUVEC, counterstaining actin cytoskeleton (green) and nuclei (blue) on our Echo Revolve #microscope
#bioscience #immunology #3Rs
@AFR_UK

Delicate red dendrites for #FluorescenceFriday - here our #animalfree Ankyron binds Tumor Necrosis Factor Receptor Superfamily member 1B (TNFR2, in red) - Nuclei counterstained with DAPI (blue) on @Discover_Echo microscope #immunology #3Rs #biomedical #research
@AFR_UK

It's February #FluorescenceFriday! Here's a beautiful image of #animalfree Ankyron™ against proliferation marker Ki67 (red) in dividing HeLa cells counterstained with phalloidin (showing actin cytoskeleton in green)
@Discover_Echo

#immunology #cells #biomedical #3Rs
@AFR_UK

It's #FluorescenceFriday! We used our #animalfree antibody replacement Ankyron™ (only 15kDa) specifically binding CD31 (PECAM1) fluorescently tagged & small enough to get into tight junctions of endothelial cells using Echo Revolve #microscope @Discover_Echo #immunology #3Rs

Banish January blues this #FluorescenceFriday and look into the lysosomes of Dendritic cells using our animal-free antibody replacement Ankyron™ binding LAMP3 (red), with nuclei counterstained with DAPI (blue). Imaging carried out with our @Discover_Echo #microscope. www.proimmune.com/ankyron/

📢 Join us next Thursday! 📢
The BSI Comparative and Veterinary Immunology Affinity Group (CVIG) is hosting an exciting online meeting. Discover the diverse applications of Ankyrons, which are next-generation binding reagents, in staining across species.
www.immunology.org/events/give-...