DjuranovicLab at Brown University RNA Center

Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes Coding mutations can cause neurodevelopmental disorders (NDDs), including autism. Yet, predicting which non-coding (e.g., 5′ untranslated region [UTR]…

Great collaboration with Dougherty and Pavlovic Djuranovic
labs on using MPRAs to look into non-coding mutations.

Approaches for identification of 5′ UTR mutations impacting translation and protein production from neurodevelopmental disorder genes
www.sciencedirect.com/science/arti...

On the way back from one week of great science, technology and therapeutic potentials of RNA in organization of Immagina Biotechnology S.r.l. and Massimiliano Clamer. Thanks for the great meeting to Max and the Immagina team. Looking forward to future collaborations.
#RNA #tRNA #ribosome

Researchers, industry leaders convene at Brown to explore the potential of RNA Brown’s Giuliani RNA Center and the Rhode Island Life Science Hub co-hosted a symposium focused on how scientists can work together to turn RNA discoveries into solutions.

A short synopsis of a recent Symposium at Brown University and inaugural Giuliani RNA Center meeting. It was an honor to be part of it.

www.brown.edu/news/2025-09...

A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies - Nature Communications Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a wide variety of cell and ti...

We are looking for a new postdoctoral fellow interested in the further development of our RAPPL technology and studies of translational complexes. Preference will be given to candidates with either structural biology or ribosome profiling backgrounds.

www.nature.com/articles/s41...

RNA Without Borders: Academic Insights, Industry Impact Join research and industry leaders in RNA science for a dynamic symposium uniting cutting-edge innovation with real-world solutions for the health of people and planet. September 26-27, 2025The Warren...

Please join us for the Inaugural Brown RNA Center Symposium with a Keynote Speakers Melissa Moore and Craig Mello, guests Victor Dzau, Jack Reed and Subra Suresh, as well as more talks and panels including both scientific and industrial speakers and panelists.

sites.brown.edu/rna-symposium/

Four days of science at
@events.embl.org
in Heidelberg for "Protein Synthesis and Translational Control" meeting.

Thanks for invitation again.

Thanks, and see you soon.

This method not only saves time, but is also economical, more efficient and mild in isolation conditions, preserving translation complexes, ribosome associated proteins and enables isolation of ribosomes for structural (cryo-EM) and functional studies. More on RAPPL uses still to come...

A rapid, simple, and economical method for the isolation of ribosomes and translational machinery for structural and functional studies Nature Communications - Here, the authors present a method to rapidly isolate actively translating ribosomes in a time- and cost-effective manner using poly-lysine. The method is compatible with a...

Our RAPPL purification of ribosomes and associated material is out. I am really proud of this study in collaboration primary with Pavlovic-Djuranovic lab, as well as Jovanovic and Hashem Labs. This method aims to replace 60 years old purification of ribosomes by sucrose cushions

rdcu.be/ezlhg

Nice work

U-rich elements drive pervasive cryptic splicing in 3’ UTR massively parallel reporter assays - Nature Communications Massively parallel reporter assays (MPRAs) are powerful technologies for measuring the impact of non-coding sequences on gene expression. Here, the authors demonstrate that MPRA reporters often underg...

Our collaboration with Tony Mustoe's lab us out today. MPRA libraries and possible cryptic splicing in MPRA reporters, or reporters in general. The additional novelty here is that such events are controlled or influenced by AU-rich sequences.

www.nature.com/articles/s41...

First RAPPL ribosome isolation at the new lab space at Brown University. Purification from a small culture of parasitic Eukaryote. Soon out...
www.biorxiv.org/content/10.1...

The Brown RNA Center | Medical School | Brown University Our mission is to advance RNA science through fundamental discovery, technology development, and translational impact that accelerates life-changing health solutions.

@djuranoviclab.bsky.social is from today officially part of Brown University and Brown RNA center. We will have our new website soon.

rna.brown.edu

Last month @uspto.bsky.social
issued yet another patent to Slavica Pavlovic Djuranovic, Jessey Erath and @djuranoviclab.bsky.social for use of polybasic peptide or non-peptide moieties against malaria parasites. More soon...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (6/6). Archaea #1

www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (5/6). Eukaryotic #3

www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (4/6). Eukaryotic #2

www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (3/6). Eukaryotic #1

www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #2

www.biorxiv.org/content/10.1...

Some of the ribosomes purified using RAPPL and Cryo-EM structures solved in last couple of months (1/6). Bacterial #1

www.biorxiv.org/content/10.1...

Itaconate modulates immune responses via inhibition of peroxiredoxin 5 - Nature Metabolism Itaconate is shown to non-covalently inhibit the antioxidant enzyme peroxiredoxin 5 in macrophages, thereby modulating the production of mitochondrial peroxide and enhancing the type I interferon prod...

This was a fun collaboration with Maxim Arttyomov lab on how itaconate inhibits the active site of a peroxiredoxin enzyme. Thanks Maxim for including us in this.

www.nature.com/articles/s42...

Ready for the next RNA session in @asbmb.bsky.social meeting. We start soon...

Ribosome data collected from a new and precious sample of 10,000,000 cells from a strange organism. All done using RAPPL, more soon...
www.biorxiv.org/content/10.1...

My suggestion to everyone is to keep some of your most valuable cell lines and back-ups in your collaborators freezers or at least in different buildings or towns.

I should point out that this was a valuable collection of mutant cell lines as well as CRISPR/Cas9 engineered lines with endogenously tagged-RBPs, translation factors, ribosomal proteins and other proteins.

3 years after terrible loss (two -80C freezers and all back-ups) of almost all of our collection of regular and mutant human, Drosophila, E. Coli and Plasmodium cells, we are still not finished with insurance.

This is how squirrel's nest looked like in our car many years ago.

Same one with L-chirality will do the same job. Adding salt will influence proteins associated with ribosomes and subunits.

One of the best single step purification preparations of protozoan ribosomes I've ever seen. Combination of RAPPL (biorxiv.org/content/10.1...) and a new technique. More at rna.ucsc.edu/rnacenter/ri...