Luc Reym⌬nd

Widefield microscope image of HeLa cells stained with SPY488-FastAct

Widefield microscope image of U2OS cells stained with SPY488-FastAct (green), PKmito Orange (white), SPY650-DNA (cyan)

Widefield microscope image of U2OS cells stained with SPY488-Fastact (green) and SPY650-DNA (red)

🟢FastAct™ goes green! 🟢🔬

We launch SPY488-FastAct™ a green channel live cell actin probe

SPY488-FastAct allows imaging F-actin in live and fixed cells

Compatible with SPY555 and SPY650 for easy 3 color imaging.

spirochrome.com/product/spy4...

available at our distributors in a couple of days. 🧪

An almond tree branch tip with flower buds nearly open and one pink and delicate flower fully opened at the tip of the branch. the background is blurry with green notes on the lower half and cyan on the upper half

Almond blossom in the garden, spring upload in full swing!

Mesmerizing actin fireworks 💥 with our actin probe SPY650-FastAct_X for #FluorescenceFriday

Easy to use: just dilute the probe in cell culture medium and image 30 min later!

🔗 spirochrome.com/product/spy6...

🙏 Big thanks to @lazaresaladin.bsky.social for the images.
🧪

An amazing preprint by @gerlichlab.bsky.social on the fate of DNA during apoptosis. Definitely to add on your reading list!

Our SPY650-DNA probe was heavily used to visualize DNA throughout the manuscript.

Congratulations to the team!

Our article on how to use micropatterned presynapses-on-glass is out in its final form in the latest issue of the Journal of Neuroscience @sfnjournals.bsky.social: www.jneurosci.org/content/46/8...
The cover went to the special section on social neuroscience, so here's our #notthecover attempt

There's actually also one in your first SiR-tubulin movie!

seems quite common. I actually found some identical structure in one image that was sent to me.

interesting!

Another fiery 💥 probes combination by @j-today.bsky.social, this time with SPY555-Tubulin and SPY650-FastAct_X.

🤩

do you know what's that MT ring structure in the lower right quadrant that seems moving?

You are a neuron whisperer, it's really looking amazing.

SPY555-Tubulin and SPY650-FastActX wound up looking good together for 11hrs. Thanks for the helpful info!

Thanks for the tip! The molecule is relatively simple so I'll stick with my peak forest, I can identify all "complications" from the 4 13C atoms present.

I think it's more of a ddtq with J1 CH of ca 50Hz then there are two J2 CH responsible of one d and one t and finally the J3 HH responsible of the quartet. Those 3 seem to have relatively similar 5-8 Hz coupling constants
A big mess 😂

that's the original quadruplet in the unlabeled molecule

About the "joy" of analyzing an 1H NMR spectrum of a 13C isotope labeled molecule when you didn't decouple 13C...

J1, J2 and J3 coupling festival!

ICYMI SPY555-FastAct_X and SiR-tubulin in action here! 🤯 16h imaging of neurons 💥
Thank you @j-today.bsky.social for posting this gem!

Let's say I had a hunch! 😊thanks for sharing.

Another wonderful system for normal phase SiO2 TLC and columns is 10 to 15% water in acetonitrile. Especially good if your compound can't handle the ammonia. I typically do gradients similar to the DCM/MeOH system.

Nice movie! How did you label MT and actin?

Cell body getting taken for a ride in this early stage neuron. Microtubules🔵 and Actin🟣 labeled and imaged for 16hr on a Zeiss LSM880. Happy #FluorescenceFriday

Thanks for highlighting our work on combining AI-based organelle dynamics detection in phase contrast with fluorescence!

www.nature.com/ncomms/edito...

@natcomms.nature.com and Nelio Rodriguez. Spearheaded by @wl-stepp.bsky.social with collaborators @jclandoni.bsky.social @maweigert.bsky.social

Powder ski day! The best of the season so far!

Smells like spring spirit!

Montage of COS7 cells stained for actin and imaged on an Apotome microscope

A menagerie of COS cells for #MicroscopyMonday 🧪

🤩 COS7 never cease to amaze me!

next level: same with live cells 😉

I witnessed the Flipper-TR development from day 1. I remember how I told myself "it can't work" when I first saw the concept. Until it actually worked beautifully! It took a decade, but now Flipper-TR is a widely used tool and an inspiring tale.
Congrats to the many who contributed to this journey!🧪

Hello epithelial mechanics fans!! I’m Juanma @juanmagararc.bsky.social 👋 I work on cell mechanics (see celldynamicslab.com) and use Flipper-TR FLIM to probe membrane biophysics in cells.

Join me on this tour about Flipper: what it measures, strengths, advice, cool case studies, and cute drawings!

Little reminder about this paper out there, it's a great reference for anyone working on Flipper-TR experiments! By @chloeroffay.bsky.social @rouxlab.bsky.social

COS-7 cell labeled for actin (gray), microtubules (red), clathrin (green) and DNA (blue). Image taken in 2018 using an OMX structured illumination microscope.

Peeking inside a cellular galaxy #cellfie 🧪

DNA-protein cross-links promote cGAS-STING–driven premature aging and embryonic lethality DNA-protein cross-links (DPCs) are highly toxic DNA lesions that block replication and transcription, but their impact on organismal physiology is unclear. We identified a role for the metalloprotease...

Our Science paper is out! 😃

www.science.org/doi/10.1126/...

Excuse my French 😂