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Pete Chuckran

@pchuckran.bsky.social

Postdoc at UC Berkeley studying soil microbes in order to avoid thinking about his own mortality

91 Followers  |  105 Following  |  4 Posts  |  Joined: 18.11.2024
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Posts by Pete Chuckran (@pchuckran.bsky.social)

The relationship between gene traits and transcription in soil microbial communities varies by environmental stimulus Codon and nucleotide frequencies are known to relate to the rate of gene transcription, yet how these traits shape transcriptional profiles of soil microbial communities remains unclear. Here we test ...

Our recent publication where the central question was: in soils, are there trademark characteristics of rapidly transcribed genes? And it was a resounding β€œit depends”. We found gene function, environmental stimulus, and phylogeny all impacted this relationship. More here:
peerj.com/articles/206...

03.03.2026 23:09 β€” πŸ‘ 2    πŸ” 1    πŸ’¬ 0    πŸ“Œ 0
RNeasy PowerSoil Total RNA Kit | Soil RNA Isolation | QIAGEN RNeasy PowerSoil Total RNA Kit is designed for the isolation of high-quality total RNA from all soil types

I've used Qiagen kits in the past which allows you to co-isolate DNA on the same spin-column.

RNA kit: www.qiagen.com/us/products/...

DNA co-isolation kit:
www.qiagen.com/us/products/...

21.01.2025 22:13 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0

Big thanks to @jeffinerca.bsky.social, @sieravirus.bsky.social, and other coauthors!

13.01.2025 21:11 β€” πŸ‘ 3    πŸ” 0    πŸ’¬ 0    πŸ“Œ 0
PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Wetting dry soil rapidly stimulates microbial activity and has important consequences for biogeochemical cycling. In our paper out today, we show that part of that response can be explained by how bacterial genomes are written (i.e. codon usage, nucleotide freq., genome size)
doi.org/10.1073/pnas...

13.01.2025 21:07 β€” πŸ‘ 35    πŸ” 10    πŸ’¬ 1    πŸ“Œ 1