Josh Quick

Rapid metagenomic sequencing for surveillance of bacterial, fungal and viral pathogens using SQK-RPB114.24 This protocol: - is a rapid method to perform metagenomic sequencing for identification of bacterial, fungal and viral pathogens. - consists of a two-in-one method that splits sample into bacterial/f...

Although SMART-9N was developed for RNA viruses, DNA pathogens can be detect either via transcripts or random amplification of genomic DNA. This is the basis of the viral arm of the @nanoporetech.com rapid metagenomic sequencing protocol (nanoporetech.com/document/rap...)

Francisella tularensis identified using @articnetwork.bsky.social SMART-9N metagenomics and @nanoporetech.com sequencing. Amazing work!

Click the link for open-source recipes for end-preparation, barcode and adapter ligation. 60% cost saving over commercial kits for the ARTIC protocol.

Ever had to pause genomic surveillance due to enzyme shortages?🫣 Happy to share our new preprint with the solution inside 🦹‍♀️💪 biorxiv.org/content/10.110…
@scalene.bsky.social @chris.primalscheme.com @pathogenomenick.bsky.social @articnetwork.bsky.social @nanoporetech.com

Changing how the world tracks emerging viruses | University of Birmingham YouTube video by University of Birmingham

And don't forget to watch the accompanying video of @scalene.bsky.social broodily walking the canals of Birmingham describing how sequencing can be useful in outbreaks: www.youtube.com/watch?embeds...

Job opportunity working with myself and @pmoyniha.bsky.social on an exciting Gates funded project developing TB lysis enzymes for rapid diagnostics. edzz.fa.em3.oraclecloud.com/hcmUI/Candid...

President Zelenskyy has been one of the greatest leaders of our time, guiding Ukraine through the darkest period of its recent history, rising up to Russia’s unwarranted aggression, being an inspiration to the Ukrainian people and ensuring that Ukraine’s voice is heard on the world stage.

Once again I am leaving the Munich Security Conference in a low mood. Amongst all the noise, the US signalled their plans for Europe, so things are becoming clearer. But things are clearly not good.
This is what we now know, and what we have to do about it:🧵1/17

Working a treat here to, threw in some melt in nut inserts etc 😊

Looking forward to that!

Oh and Josh, I just wanted to let you know that I refer to your excellent 'lab-in-a-suitcase' work as a primary example of situations where the DIYNAFLUOR may find utility. It typically shows up in the first 1 or 2 slides of any presentation I make. 😅

For ONT libraries being able to measure in the range 1-100ng/ul is perfectly adequate.

A colleague here and I had an idea to use a dye with a larger Stokes shift which might help with some of the scatter but I honestly don’t think it’s necessary now.

I had been wanting to do this for a while. I got as far as dismantling one and measuring the spectra of the filter set.

This will be huge for LIAS because the fluorometer was one of the most expensive bits. Qubit 4 currently £2800 on our procurement system!

Built our first two DIYNAFLOURS and they work great! Thanks @willaanderson.bsky.social

New HPAI genotype found in Nevada dairy cows APHIS confirms first detection of D1.1 genotype in dairy cattle through NMTS testing.

Genotype D1.1 of H5N1 confirmed in Nevada cattle, which is the one circulating in birds and not B3.13, causing the wider cattle epizootic in the U.S.

In other words, this is a second observed jump from birds into cows.

Keep rolling the dice, shall we?

www.feedstuffs.com/agribusiness...

Leeds becomes first Russell Group university to quit X - Research Professional News Leeds says Elon Musk’s social media site “no longer aligns with our university values”

The @universityofleeds.bsky.social is the first Russell Group Uni to quit X! 🎉 🎉

Read more👇

tinyurl.com/2h3tyjfx

@lsjleeds.bsky.social
#Yorkshire #Leeds #Academia #AcademicSky #HigherEd #LawSky

YouTube video by Led By Donkeys Heil Tesla

The world’s richest man is promoting the far right in Europe. Don’t buy a Tesla
.
Location: Tesla Gigafactory, Berlin

www.youtube.com/watch?v=NjWl...

President Trump will learn pretty soon that Putin cannot be convinced with money, at least not any more. He is the richest person in Russia already, though he will never say no to more billions, but that wasn't the point of him starting the full scale war in Ukraine. (Thread)

Same here, had the flu last week.

Any error message?

Turns out it works for bacteria too, can’t believe we didn’t try that! bsky.app/profile/nath...

Can’t wait to see what people do with it. Merry Christmas!

Massive effort by @chris.primalscheme.com and @aresti.primalscheme.com check it out now at primalscheme.com

The latest version will perform sequence alignment and design primers can amplify whole viral species. It can also make massive schemes with thousands of primers.

Really glad this is out there; a lot has happened in the 7 years since the original paper including:
-67,000 schemes designed by the community
-18M genome sequences using PrimalScheme

PrimalScheme: open-source community resources for low-cost viral genome sequencing Viral genome sequencing using the ARTIC protocol has been a vital tool for understanding the spread of epidemics including Ebola, Zika, COVID-19 and Mpox and has seen widespread adoption due to its lo...

Fancy some light Christmas reading? I’m happy to share our new preprint, covering the impact and cutting edge improvements to PrimalScheme!

Preprint: www.biorxiv.org/content/10.1...

If you can wait a few hours we have a preprint in the BioRxiv queue!