Sheyda Azimi

Check out the Microbial Primers page. Short articles written to help people new to a field to get quickly up to speed. @microbiologysociety.org

www.microbiologyresearch.org/content/micr...

Nice blue!

We combined whole tissue clearing with HCR-mRNA FISH imaging to differentiate and visualize different PAO1 variants in mouse lungs infected with mixed populations.

Whole-tissue imaging reveals intrastrain diversity shapes the spatial organization of Pseudomonas aeruginosa in a murine infection model | mSphere Intrastrain genetic and phenotypic diversity within Pseudomonas aeruginosa populations is common in chronic pulmonary infections. While this intrastrain heterogeneity is a hallmark of chronic infection, its consequences for the spatial organization of P. aeruginosa within the airways remain unclear. Here, we demonstrate that the loss of O-specific antigen in a subpopulation of P. aeruginosa significantly alters the spatial architecture of P. aeruginosa, without changing the total population size or composition. Using a combination of tissue clearing and hybridization chain reaction RNA-FISH in a murine lung infection model, we mapped the localization of genetically distinct P. aeruginosa variants in mixed populations in vivo. These findings reveal that genetic diversification within a strain can reshape the infection landscape at the micron scale, highlighting the overlooked role of intrastrain dynamics in shaping the microbiogeography of infections and influencing host-pathogen interactions.

Recent work from the lab showing how presence of O-antigen deficient Pseudomonas variants change aggregate assembly and microbiogeography of infection

journals.asm.org/doi/10.1128/...

I usually get the heated laptop support late at nights! 😊

This was a fun semester, fun times teaching Advanced Molecular Cell Bio with @kswetzel1.bsky.social to first yr Bio PhD students at GSU!

JB Editor's Choice: Anderson, Khursigara et al. review the role of peptidoglycomics in understanding the structure and function of peptidoglycan.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

Such a Friday, double win at Biology Department with Bio for Breakfast and Research day @jamesgurney.bsky.social @persisterbritt.bsky.social @kswetzel1.bsky.social

So far during fall semester at Bio for Breakfast series at GSU!
@kswetzel1.bsky.social

My first hour at work #mycyclingtoworkera

So amazing…

Top: Schematic of GP2 filaments, with labeled residue ranges of ZP domains and four N-glycosylation sites. Middle left: Cryo-EM map of GP2 filaments, color-coded according to the schematic above. The density of one of the flexible branches was indicated by a black arrow. Middle right: Atomic model of GP2 filaments, with five N-glycosylation sites marked by arrows. Bottom: Proposed model of GP2 filaments’ function in the digestive tract. The filament core of UMOD and GP2 is colored in dark blue and green, respectively. The EGF domains and D8C/D10C domains in the branches of both proteins are shown in grey and light blue, respectively. High-mannose N-glycans are colored in red. UPEC cells and their adhesive pilus are colored in pink, while the proteases (such as elastase) are colored in yellow.

#Glycoprotein 2 (GP2) filaments protect against infections by acting as decoys for the bacterial #lectin FimH. The #cryoEM structure of GP2 filaments provides insights into filament assembly and #antibacterial function in the digestive tract @plosbiology.org 🧪 plos.io/3HP2obF

Postdoc alert!! Rolf is an amazing advisor and Zurich is a great place to live. Send your best students this way! ⬇️📢 #MicroSky 🦠❤️

Day 2 of the retreat also means a lobster dinner, a view of the light house and some blueberry pie!

New review on Pseudomonas aeruginosa by @digglelab.bsky.social and Marvin Whiteley, and Morgana Letizia

When you're back in your hotel room after a full day of talks and it's time to go to the concluding conference party

I’m sorry and I hear you! By pet photos, you mean cats right?

Heterogeneity in recombination rates and accessory gene co-occurrence distinguish Pseudomonas aeruginosa phylogroups | mSystems The consummate opportunist Pseudomonas aeruginosa inhabits many nosocomial and non-clinical environments, posing a major health burden worldwide. Our study reveals phylogroup-specific differences in r...

Good Pseudomonas aeruginosa comparative genomics study by @reeskassen.bsky.social &co journals.asm.org/doi/10.1128/...

Georgia State Day 2025 | Donate Giving Day Powered by ScaleFunder

Consider supporting Panthers at GSU on Georgia State Day! thestateday.gsu.edu/giving-day/1...

Amazing! Missing the @microbiologysociety.org meetings!

Please repost: Postdoc position in antibiotic discovery in a large multidisciplinary project combining high-throughput approaches, microscopy, AI, and hit-to-lead optimization at @umontreal.ca and @mila-quebec.bsky.social. Apply to ML.Antibiotics@gmail.com. See
lnkd.in/dVVk4N3s. #microsky

Dear #microbiology community,

Great news regarding the 2025 Mechanisms of Microbial Transcription GRC/GRS (US):

We as chairs succeeded in securing more funds for trainees ! 😃💰
- Full & partial registration reimbursements and partial travel support are available!

r u joining us in June? #microsky

New preprint! How S. aureus copes with inhibitory compounds secreted by P. aeruginosa. By @schluk.bsky.social. Great collaboration with @fgcz-en.bsky.social
www.biorxiv.org/content/10.1...

JB Table of Contents Volume 207, Issue 2 Journal of Bacteriology: Table of Contents Volume 207, Issue 2

The new JB is out. We thank ad hoc reviewers for 2024, minireviews on spores, mycobacteria, phages in the gut, formate metabolism and biofilm microenvironments. Check out articles on LD-transpeptidases, Myxo, B12 (my favorite vitamin) & more.
journals.asm.org/toc/jb/207/2
@asm.org #JBacteriology

Summer School, Quantitative Phage-Bacteria Dynamics Across Scales U of Maryland, College Park, June 23-27, 2025

A small moment of science in the world of the very small... announcing calls for applications from MS + PhD students + postdocs for a Summer School on

Quantitative Phage-Bacteria Dynamics Across Scales
U of Maryland, College Park, June 23-27, 2025

Apply by 3/21/25:

bit.ly/phageschool2...

We are excited to announce a new faculty position here in Cambridge, for researchers in computational and/or theoretical biology, based jointly in Genetics and Mathematics. Come and join us! Happy to answer questions about research, teaching and working here. www.jobs.cam.ac.uk/job/50414/

I’m an NIH-funded researcher, drowning in uncertainty “I’ll keep doing what I can to move my research forward. But I, and so many others, can’t do this indefinitely,” this assistant professor writes

In my latest article for @science.org, I reflect on the current challenges faced by early-career researchers, including delays in grant reviews, uncertainty around NIH funding freezes and changes, and the critical need for continued support and advocacy.
www.science.org/content/arti...

JB Editor's Pick: Pickens, De Leon et al. describe the distinct contributions two critical biofilm adhesins for SRB.
journals.asm.org/doi/10.1128/...
@asm.org #JBacteriology

Fridays are “Fundays” at GSU Biology! This morning @persisterbritt.bsky.social gave an overview of her research plan at Bio for Breakfast!
@peachypotato.bsky.social