Petr Cejka

November magic in Graubünden

Among the anti-recombinases, FIGNL1 rules them all. So much that inactivating it brings BRCA2-deficient cells to life. Who is responsible for RAD51 loading without BRCA2/FIGNL1, check out the paper to find out! Great collaboration with @raychaudhurilab.bsky.social

www.science.org/doi/10.1126/...

See our paper “Mechanism of trinucleotide repeat expansion by MutSβ-MutLγ and contraction by FAN1”. Using biochemistry, we show how DNA incisions by the MutLγ nuclease can lead to expansions, and how expansion is prevented by FAN1. Well done Issam and Valentina!

www.nature.com/articles/s41...

Lake Como with plenty of fall sunshine today

Golden October in its glory. Mountains in Ticino today.

Switzerland

Trip to Greina plateau, northern Ticino

Last hike in August with first snow

Image of Toronto

JOB ALERT 🚨 We are hiring TWO principal investigators in cell, molecular, systems, or chemical biology in Toronto, Canada at @sinaihealth.bsky.social. We provide a generous startup, fully funded salary and academic appointment at U of Toronto.

www.nature.com/naturecareer...

Please repost!

Hiking in Switzerland today, Val Poschiavo

Please see our latest paper on the role of EXO1 in meiosis: "EXO1 promotes the meiotic MLH1-MLH3 endonuclease through conserved interactions with MLH1, MSH4 and DNA". Congratulations to both first authors, Megha Roy and Aurore Sanchez and thanks to all our collaborators!

Southern Alps today

ATM priming and end resection–coupled phosphorylation of MRE11 is important for fork protection and replication restart | PNAS The MRE11/RAD50/NBS1 (MRN) complex plays multiple roles in the maintenance of genome stability. MRN is associated with replication forks to preserv...

🧬 New research discovers sequential phosphorylation of MRE11 by ATM and ATR prevents extensive DNA degradation at stalled replication forks
www.pnas.org/doi/abs/10.1...

Nice weekend everyone from sunny Bellinzona

Great news, thank you

IFI16 senses and protects stalled replication forks Replication stress is a key driver of DNA damage and genome instability. Replication stress-induced fork remodelling generates a new DNA end that is vulnerable to the action of nucleases, and which is...

New preprint from the lab, revealing new roles for IFI16 during replication stress. Great collab with @unterholznerlab.bsky.social and @cejkalab.bsky.social

www.biorxiv.org/content/10.1...

Spring is here. Valleys and lakesides are green, and snow is melting fast in the mountains.

Mechanisms and regulation of DNA end resection in the maintenance of genome stability - Nature Reviews Molecular Cell Biology DNA end resection is crucial for most DNA double-strand break repair pathways. This Review discusses the molecular mechanisms of end resection and its regulation, focusing on the roles of post-transla...

ICYMI - New #Review on #DNARepair

In this Review, Raphael Ceccaldi and @cejkalab.bsky.social discuss the mechanisms and regulation of DNA-end resection, which is crucial for the repair of DNA double-strand breaks by most repair pathways.

🔗 go.nature.com/4iSKphQ

Are they related?

An interesting collaboration with the @vindignilab.bsky.social on the mechanism of resection at gaps. Spoiler alert: the MRE11 complex acts very differently at gaps vs. at DNA breaks genesdev.cshlp.org/content/earl...

Check out our review on DNA end resection! With Raphael Ceccaldi, www.nature.com/articles/s41...

Exciting seminar series from Genome integrity italian Network (GiiN), chaired by myself and Raffaella Di Micco! Short talks (2x30’), at convenient time for EU/USA attendees. Big shots and raising stars in an expanding field. See flyers + link to receive email alerts. Please repost to your network!👇

Structural basis for DNA double-strand break sensing by human MRE11-RAD50-NBS1 and its TRF2 complex The MRE11-RAD50-NBS1 (MRN) complex is a central, multifunctional factor in the detection, signaling and nucleolytic processing of DNA double-strand breaks (DSBs). To clarify how human MRN binds generi...

Happy to share our latest pre-print!
We solved the cryoEM structures of human DNA repair factor MRN bound to DNA and to TRF2. Main work of Yilan, Filiz @filizkuybu.bsky.social and Hengjun. Great collab within the @sfb1361.bsky.social and @genecenter-lmu.bsky.social 🧬🔨
www.biorxiv.org/content/10.1...

Looks like an amazing achievement - looking forward to reading it!!!

If you are interested in mitochondria, Nashville is the place to be June 1-5. Check out this FASEB meeting. Please RT. events.faseb.org/event/mitoch...

Very sad to read about the science funding situation in the US at the moment. I am waiting for some funding decisions in the coming weeks, but I will likely have opening(s) in my lab in Switzerland in the coming weeks (PhD student/postdoc). As a PhD candidate, you need to have a Master's degree.

What a sad situation. I am waiting for some funding decisions on my end, and may have opening (s), so please let them know

Understandable, but so unfair - sorry….

Congratulations!!!!

Or cold room, after protein purification (when it fails…)