Chlanda lab

Our latest preprint is now available doi.org/10.1101/2025...! Led by my postdoc, Luqiang Guo @luqiang.bsky.social, we determined a ~2.4 A resolution structure of the hantavirus glycoprotein tetramer on the surface of VLPs. Also determined structures of dimers of tetramers and Fab-bound tetramers.

www.biorxiv.org/content/10.6...

Structural basis of caveolin-driven membrane bending

Afterwards a panel discussion was moderated by Caroline Schlaufer featuring film director Christian Frei, biosafety expert Kathrin Summermatter & virologist @profvolkerthiel.bsky.social. The audience posed great questions on funding, biosafety & media polarisation.

📢Job alert📢

@uniheidelberg.bsky.social has an opening for a full W3-position on “Modeling of Biological Processes” 😀👍 Here is the call

tinyurl.com/3w9e9dtf

and application deadline is March 15. The group would be located at the beautiful #BioQuant center right in the middle of Neuenheimer Feld.

Trapozoid cryo-FIB milling allows to prepare a lamella for dual-axis cryo-ET and assessment of different deep-learning missing-wedge restoration algorithms. rdcu.be/e2nXE

Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET Nature Protocols, Published online: 30 January 2026; doi:10.1038/s41596-025-01284-zA protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.

New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET

Check out our new preprint! We uncover the full molecular mechanism of rotavirus membrane penetration and cytosolic escape using cryo-ET, live-cell imaging, and single-molecule assays. (1/3)

🔗 biorxiv.org/content/10.6...

Cryo-EM structure reveals how influenza A virus NEP binds the viral polymerase at a regulatory hotspot, coordinating RNA synthesis and nuclear export. Fantastic collaboration with @loiccarrique.bsky.social and Jon Grimes. www.science.org/doi/10.1126/...

Cryo-electron tomography reveals coupled flavivirus replication, budding and maturation - Nature Communications In this study, Dahmane et al use a method called cryo-electron tomography to uncover new details of how tick-borne flaviviruses transform cells into virus factories.

I am super happy to share this paper in its final form. We used FIB milling to "dig in to" cell lines and mouse brains infected with tick-borne flaviviruses, followed by cryo-ET to study the virus replication. It's open access, so have a look!
#virology #teamtomo
www.nature.com/articles/s41...

Open #postdoc fellowship in my group. Virus replication and cryo-ET. Projects can be tailored to ambitious candidates with diverse backgrounds, so don't hold back from applying! DM for more info.

Deadline 8 Feb. Apply here:
umustipendie.varbi.com/en/what:job/...
#virus #virology #teamtomo #cryoEM

Sharing this here at the request of Dr. Florian Krammer:

semmelweisinstitute.ac.at/training-car...

A new path to spillover: MHC-II entry of influenza A viruses Spillover of influenza A viruses from animals to humans represents a threat to our health. This Perspective discusses emerging research that suggests some influenza A viruses can enter host cells via…

Spillover of #influenza A viruses from animals to humans represents a threat to our health. In this Perspective, Silke Stertz @virology.uzh.ch discusses emerging research that suggests some influenza A viruses can enter host cells via MHC-II receptors across species 🧪 #virology plos.io/4pP3Gnq

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

Our new preprint is out 🥳🥳🥳

Henipaviruses, like Nipah and Hendra, package their genomes inside helical shells built by thousands of nucleoproteins. These nucleocapsids are essential to protect the viral RNA, but how do they ever let the polymerase in to read the sequence?

👇

Please RT. Post-doc opportunity alert! 💥 closing 10th December.. Come join our team (www.thelowlab.org) at Imperial, London, working on the structure and mechanism of bacterial secretion systems.

For more details and to apply please see

www.imperial.ac.uk/jobs/search-...

warwick.ac.uk/fac/cross_fa...

I am advertising for a PhD position in my group. We'll be investigating how pathogenic RNA viruses replicate in the nucleus. The project will teach cryoEM/ET, virus culture, and in vitro assays.

If you're interested reach out and I'd be happy to have a chat.

Thank you, Lars, for the invitation and for the opportunity to share the work. It was a great meeting and also a very nice opportunity to go again to beautiful Umeå!

Mysteries of trafficking of the vRNP segmented genome and assembly of influenza A virus revealed by in situ cryo-ET!
🔗 rdcu.be/eMmct
We are very excited that our paper is finally out!
🎉 Big congratulations to Moritz Wachsmuth-Melm and to everyone involved.

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...

“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling | Science www.science.org/doi/10.1126/...

Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids.
Link: www.biorxiv.org/content/10.1...

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit
www.nexperion.net/semcm2026

Illuminating druggable dark matter in RNA virus replication using in situ cryo-EM Viral proteins typically exist in the context of complex virions or in the even more complex host cells in which they replicate. Hence, meaningful ins…

Since it was published at peak holiday season, I'm taking the liberty to re-introduce our recent review article. We hope it will be a useful overview of RNA virus replication strategies, and how in situ cryo-EM have helped us understand them:
www.sciencedirect.com/science/arti...

Sub-cellular chemical mapping in bacteria using correlated cryogenic electron and mass spectrometry imaging

Congrats Hannah Ochner and authors on this important paper! Strong collaboration with @kiranrpatil.bsky.social

www.biorxiv.org/cgi/content/...
@mrclmb.bsky.social @wellcometrust.bsky.social

PhD student in cryo-ET of membrane trafficking at MPI-NAT in Göttingen, DE Post a job in 3min, or find thousands of job offers like this one at jobRxiv!

One week left to apply for a fully funded PhD position in my group! Please repost. jobrxiv.org/job/max-plan...

An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission - Nature Microbiology A unique Arp2/3 complex in malaria parasites enables DNA segregation during the rapid formation of male gametes by safeguarding the attachment of chromosomes to the mitotic spindle.

Awesome malaria study out now in Nature Microbiology:

An atypical Arp2/3 complex is required for Plasmodium DNA segregation and malaria transmission

By Franziska Hentzschel, Matthias Marti, Freddy Frischknecht & colleagues!

@freddyfrischknecht.bsky.social

www.nature.com/articles/s41...

Influenza filaments: a graveyard of postdocs no longer? | The EMBO Journal EMBO Press is an editorially independent publishing platform for the development of EMBO scientific publications.

Really enjoyed writing about one of my favorite topics (influenza filaments). There is so much exciting new filament research now, it is clearly no longer 'the graveyard of postdocs' Bob Lamb called the topic during my PhD. www.embopress.org/doi/full/10....

Very nice News&Views summary written by
‪@brucelab.bsky.social‬ about our work and work by Ed Partlow et al. (NIH) that influenza A virus filamentous morphology plays an important role in infection (www.nature.com/articles/s41...) can.
‪Thank you!
(www.embopress.org/doi/full/10....)

We are happy this work by Sarah Peterl et al. is out in EMBO J! It was an exciting collaborative effort of several groups in the SFB1129 consortium on pathogen replication and spread. Check out the correlated light and scanning EM method to study virus morphology changes during spread!

Very well deserved, congratulations, Tanmay!

Molecular basis of influenza ribonucleoprotein complex assembly and processive RNA synthesis Influenza viruses replicate and transcribe their genome in the context of a conserved ribonucleoprotein (RNP) complex. By integrating cryo–electron microscopy single-particle analysis and cryo–electro...

Excited to share our new @science.org paper! Led by postdocs Ruchao Peng and Xin Xu, we used cryo-EM/ET to reveal the influenza ribonucleoprotein complex structure and its strand-sliding mechanism for RNA synthesis, paving the way for new antivirals.
www.science.org/doi/10.1126/...