Hannah Rae Thomas

Group Leader in Discovery Plant Sciences | John Innes Centre The John Innes Centre (JIC) seeks a visionary Group Leader to lead research on Discovery Plant Science.

. #Botany2025 My institution (JIC in Norwich, UK) is hiring for a tenure-track Group Leader in Discovery Plant Sciences - see more here:

www.jic.ac.uk/vacancies/gr...

Let me know if you have questions!

Pleased to have finally got this method to work in wheat! Find out how you can identify direct targets of transcription factors in wheat using protoplasts and the TARGET method:
dx.doi.org/10.17504/pro...
A very useful method for our group to use! borrilllab.com

#plantbio2025
Amazing talks this morning!

Me too! 😆

We focus on the integration of #light, #temperature, and #auxin during herbaceous grafting. This review highlights recent advancements and spotlights gaps in our understanding! Please enjoy!

Interested in how abiotic factors regulate plant #grafting? Check out our new review in Planta!
link.springer.com/article/10.1...

Front cover of a postcard to promote an upcoming special issue of the Journal of Experimental Botany titled "Plasmodesmata". The issue will be edited by Tessa Burch-Smith and Jacob Brunkard. The images show the following. Above: silencing signals move out from veins through plasmodesmata in a Nicotiana benthamiana leaf (Jacob Brunkard); Centre: fluorescent Protein moves from a central transformed cell to neighbors through plasmodesmata (Jacob Brunkard); Far right: electronic microscopy reveals nanoscopic structure of plasmodesmata, shown in gray (top) or blue (bottom) (Tessa Burch-Smith; Tina Schreier).

** UPCOMING SPECIAL ISSUE **

Plasmodesmata

Editors: Tessa Burch-Smith & Jake Brunkard

Deadline: 1st Feb 2025

Manuscripts of any format welcomed, please contact the JXB office (bit.ly/JXBissues)

#JXBspecialissues 🌱 🔗 🌿 🧪 #plantscience

@pdchristine.bsky.social @tinaschreier.bsky.social

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At least the colors are festive? 🫣

I'm planning to attend #PlantBio2025 with a few first time US visitors. What #Milwaukee -based or nearby sites would you prioritize for a few days of fun?

PhyloPic PhyloPic is an open database of free silhouette images of animals, plants, and other life forms, available for reuse under Creative Commons licenses. Download silhouettes for use in educational materi...

Ok people. I’m drawing silhouettes this afternoon. If you have a paper coming up and you are in need of a #silhouette please let me know and I will have it ready today. As always I upload all my silhouettes to phylopic.org

I also have tutorials on how to put a silhouette in a plot in R.

I'm so excited about my new #orchid. What are some tips to make sure I don't kill it?

Title slide: Will your code run again? Tips for making code reproducible in R

If you missed my talk but still want to learn how to make your R code more reproducible, my slides are here 🙂:

daxkellie.quarto.pub/will-your-co...

All the links to packages and resources I mentioned are there, so hopefully this can be a nice reference, too!

#ESAus2024 #rstats #quartopub 🧪🌏

Devito meme where he's shooting and saying "So anyway I started Blasting". Image modified to haver the Altmetric badge in front of Devito's head, and the caption now reads "So anyway I started Tracking Bluesky". The date is given as the 3rd December 2024

Amye Kenall, VP Product, Data & Analytics Hub, Digital Science, said: “Bluesky has become a favored social media channel for research communications professionals and academics. Indeed, in our own research we found over 22% of researchers were using Bluesky over any other social media channel."

Single photon imaging of three capsicum leaves. Leaf 1 is a control. Leaf 2 and 3 are harboring a bioluminescent bacteria growing under different conditions.

When the first experiments in the lab worked! And... the data was significant. 💃💃💃#newPI

I mean... he kind of gassed you up. 😅 I'm impressed with your superpower. 😉

Happy Friday everyone! Here are some cool Arabidopsis mini trees🌴

Arabidopsis arenosa hypocotyls can undergo extensive secondary growth and the rosettes that develop on top make them look like palm trees 🤯I wonder if this can be engineered in thaliana..

🤩🤩🤩 I want to see a cross section so bad!

Spending time here on the blue place has been so nice lately. 🥹

Yes, this is likely what we will need to do.

It's about 150 bp downstream in the first exon.

I'll definitely look into it! 👀

Yes we check protein levels in OE🙈. We'll check for any off targets in the crispr. What a mess! Hahahaha

Would cosuppresion be consistent in all tissue types? Or do you think it could vary?

Yes, we get a frame shift but transcripts map to the entire gene (besides the deletion site). OE is about 10x higher than WT. Maybe being suppressed? Occams razor would make me think it isn't 2 strange things going on, but who knows!

Seems possible! Then the antibody would still bind!

Deletion is in the middle of the first (or three) exons. Phenotype is fruit ripening timing.

I suppose it's always a possibility. Seems like there would have to have been several mix ups. 😬

It could be a possibility! Phenotype is fruit related. I'll double check that all the experiments were in fruit and not leaf! Would anything explain the protein situation. 🤔🤔

Im totally baffled and hoping the collective mind might have some ideas! 🙏

We have a crispr line w/ 5bp del and phenotype. We can see the edit in gDNA seq, but reads mapped + RT-PCR levels are normal, and the protein can be detected at (we think) full protein mass.
In the OE, reads are higher but phenotype look like crispr pheno?
What could be going on. Any thoughts? 🤡