Mick Craig

My wife has already claimed this spot... 😂

Almost: if I'm not moved enough to feel like singing or composing a poem, the cheese has failed to achieve the desired effect.

November means it's time to start auditioning cheeses to see which ones can earn their spot on our Christmas dinner table... 🤤

Definitely worth a bottle if you like your whisky to have a woody / oaky edge.

I really like the virgin oak bottling from these guys - remniscent of the Auchentoshan Three Wood but substantially cheaper.

A chicken neural crest explant with neural crest marker sox9 in green, e-cadherin in orange, filamentous actin in magenta, and nuclei in gray.

Today for #FluorescenceFriday I’m sharing a 👻Halloween-themed🎃 neural crest explant “web of cells” from Julia Godinez, a 4th year @ucdavis.bsky.social graduate student in the lab. She is studying mechanisms driving conserved and divergent cranial neural crest migration and differentiation. #DevBio

*The uninvited guest* (sound on this time!)
Third week of observing scary things under the microscope: watching #SARSCoV2🟣 positive cells arrive in the brain. Note it's still inside a blood vessel and being checked by astrocytes🟢and microglia🔵. What happens next? 😨 #FluorescenceFriday

Image captured at 4X on a fluorescence microscope showing a tissue section through the mouse prefrontal cortex where neurons that project to the locus coeruleus in the brainstem are virally labeled in red and neurons that are activated are labeled with Fos in green.

For #FluorescenceFriday - projection neurons from the mouse prefrontal cortex to the locus coeruleus 🔴 with Fos labeling activated cells 🟢. Image credit to the Circuits team in the Translational Neuroscience Division @lieberinstitute.bsky.social 🧠🔬🧪

Monosynaptic rabies tracing from deep neurons in retrosplenial cortex. Cyan shows deep neurons labelled with GFP, magenta shows retrogradely-labelled neurons and white cells will be starter cells.

Another #FluorescenceFriday image here, from an ongoing project studying circuitry of the retrosplenial cortex. Here we are using rabies to map the inputs specifically to the deep pyramidal cells (L5). Cyan shows the deep cells and magenta shows their presynaptic partners. #NeuroSkyence

The astrocytic ensemble acts as a multiday trace to stabilize memory - Nature Emotional experience evokes signalling in astrocytes, which form an ensemble that is reinforced by secondary astrocytic state changes resulting from repeated experience, leading to memory stabilizatio...

𝗘𝗻𝗱 𝗼𝗳 𝘁𝗵𝗲 𝗲𝗺𝗽𝗶𝗿𝗲 𝗼𝗳 𝗻𝗲𝘂𝗿𝗼𝗻𝘀 𝗶𝗻 𝘁𝗵𝗲 𝗯𝗿𝗮𝗶𝗻??
Astrocytes in the regulation of fear memories!
www.nature.com/articles/s41...
#neuroskyence
h/t @claeneuro.bsky.social

Finally, someone has solved a real problem with AI! No more having to take a paper in the format for a journal that rejected you, and reformat it for a new journal. Well done!! formatmypaper.com

Thanks Mike, it's such a relief to finally get this across the line, given that I already knew the key result in 2014! Hell, I first stuck channelrhodpsin in reuniens during my PhD back in 2008 when such things were becoming cool (trying to use it to turn off network activity in entorhinal cortex).

Monosynaptic retrograde tracing from hippocampus proper shows no cells originating from nucleus reuniens (NRe), unless the starter cells are present in subiculum region. Left: Injection site for dorsal CA1 and viral spreads for the helper virus (green cells). B and D, Injection site of a dCA1 injection: green cells contain helper virus and red cells are pseudotyped rabies virus positive and starter cells (yellow) shown closer on the inset, shown by arrowheads. C and E, No cells found in any thalamic section containing NRe.

Excitatory input from #PrefrontalCortex to #hippocampus pyramidal cells is believed to be mediated through the thalamic nucleus reuniens. @mickcraig.bsky.social &co show in rodents that this brain pathway is unexpectedly minor, contrary to assumptions in the field @plosbiology.org 🧪 plos.io/48u6mle

I would also take a look here for some more recent rodent evidence of reuniens playing a re in extinction of fear memory: www.nature.com/articles/s41...

This is a great question! We've never really studied fear memory in our lab but @memorycontrol.bsky.social has done a lot of thinking on the potential rule of reuniens in fear memory. This is a great place to start - www.memorycontrol.net/2021Anderson....

Thanks man, appreciate that! 🙏

I'll say it: I think its rly annoying and frankly amateurish that writers are constantly told their villains must be understandable or have sympathetic motivation to be interesting or realistic when all our big villains irl are in fact rich, stupid, selfish, trickster dillweeds and always have been

Hippocampal pyramidal cells of the CA1 region are not a major target of the thalamic nucleus reuniens Excitatory input from the prefrontal cortex to hippocampus pyramidal cells is commonly believed to be mediated through the thalamic nucleus reuniens brain region. This study shows in rodents that this...

Delighted that our main #TeamReuniens paper is finally out in @plosbiology.org. Been a long time in the making, congratulations to Lilya and all the team. A thread to follow soon, after I've had some sleep... #NeuroSkyence

journals.plos.org/plosbiology/...

Did a slightly early Thanksgiving meal today and my pumpkin pie set! I take a somewhat freestyle approach in the kitchen so have a patchy track record with pumpkin pie, but I actually read a recipe and measured a little today. Enjoy tomorrow for those to celebrate! 🍁🇨🇦

Brain-Computer Interface Allows Man With ALS to ‘Speak’ A brain-computer interface (BCI) developed by UC Davis Health accurately translates brain signals into speech. The device implanted in the brain of a man with ALS is the most accurate system of its ki...

No comment on what THIS company is doing because (*shudder*), but if you want to follow REAL scientists on this look at groups like: www.ucdavis.edu/news/brain-c...

publicly 👏 funded 👏 research 👏 changing 👏 lives 👏

Craig Lab Translational neurobiology

I finally made a lab website, a mere 9 years after starting... oops. It's still a bit rudimentary but it'll do for now... craiglab.org

From the CringeTikToks community on Reddit: Brutally spot-on. 😳👇 Explore this post and more from the CringeTikToks community

Not going to lie, this is spot on and frankly, yeah, this IS how I feel and if I wasn't clear, placing this because she did it better than I would have and to be certain, in less time. 😜

If this is #cringe, then cringe away because I'm not.

Ventral hippocampal pyramidal cells transduced with rabies (cyan), helper proteins (magenta), or both. Part of a monosynaptic circuit tracing experiment

Are we still doing #FluorescenceFriday? #NeuroSkyence

For Starmer and Labour the point is to beat Reform in elections. Maybe this will work, to that end.

But for the public and the nation, the important thing is to defeat the fascism and its racism. And this approach will achieve the very opposite

Details on how to apply can be found here: www.gla.ac.uk/postgraduate...

Fully funded #FindAPhD! We'll be studying neurobiology of motivation in mice hunting crickets, using a combination of genetic manipulations, behaviour and in vivo ephys. Co-suprvised by the amazing @agonru.bsky.social and Gareth Arnott at QUB. #Neuroskyence Pls share

www.gla.ac.uk/postgraduate...

Damn, this must have been quite the faff! Congratulations to you and the team 💪💪

GABAergic circuits reflect different requirements for sensory integration in postnatal mouse neocortex Information transfer in mammalian cerebral cortex is dependent on GABAergic interneuron circuits that are widely assumed to be uniform across the neoc…

A rather 'unique' paper from our lab is online in @cp-cellreports.bsky.social now. Unique in the sense that I don't think anyone else will be rushing to optotag cortical interneurons in the early postnatal brain... because it is nigh on impossible!

www.sciencedirect.com/science/arti...

library(tidyplots) study |> tidyplot(x = treatment, y = score, color = treatment) |> add_mean_dash() |> add_sem_errorbar() |> add_data_points() |> add_test_pvalue(comparisons = list(c(1,3),c(2,4)))

This is how you can test selected comparisons in #tidyplots 0.3.1 📋

#rstats #dataviz #phd

Machiavellan. Love it 😂