Bruno Di Stefano

Dr. Bruno Di Stefano (Bruno Di Stefano) Stem Cell Reports Early Career Editor , will be at the American Society of Hematology Annual Meeting in Florida, USA 6-9 December 2025. Connect with him to discuss whether your research is fit for publishing in Stem Cell Reports!

Elevate your career in stem cell science! Become an ISSCR member to enjoy exclusive benefits: complimentary webinars, preferred rates for events including ISSCR 2026, publishing benefits, and access to an international network. Join today 👉 https://bit.ly/4eOL2H1 Bruno Di Stefano

Meet Early Career Editor Bruno Di Stefano (Bruno Di Stefano) at #ASH2025 in Orlando, USA 6-9 December 2025. Connect with Dr. Di Stefano to discuss whether your research is a fit for publishing in Stem Cell Reports!

Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology Stem cell differentiation is controlled by manipulating RNA condensates.

Selective RNA sequestration in biomolecular condensates directs cell fate transitions - @distefanolab.bsky.social @brumbaugh-lab.bsky.social go.nature.com/3X215du

8/ Huge thanks to our editor and reviewers for invaluable feedback, and to our funders NIH, @ash.hematology.org , @worldwidecancer.bsky.social, @bepositivefdn.bsky.social, Webb-Waring Biomedical Research Awards, and CPRIT for supporting this work!

7/ Our findings establish a fundamental, conserved role for P-bodies in cell fate specification and reveal novel strategies to manipulate RNA condensates for directing cell identity in regenerative medicine.

6/ Applying these insights to stem cell differentiation, we show that manipulating P-body assembly or specific microRNA activity can direct pluripotent stem cells toward totipotency or the germ-cell lineage.

5/ What drives the selective sequestration of mRNAs in P-bodies? We found that certain cell-type–specific microRNAs are enriched in P-bodies and modulate RNA sequestration. Disrupting microRNA–target interactions prevents the corresponding mRNAs from being sequestered.

4/ Dissolving P-bodies in mouse naïve ESCs increased ribosome occupancy of P-body–enriched RNAs, including 2C transcripts, triggering reversion to a 2C-like state. This shows that releasing RNAs from P-bodies enables their translation and drives cell fate transitions.

3/ Surprisingly, P-body contents don't simply reflect current gene expression but are enriched with transcripts from the prior developmental stage. For example, in human naïve pluripotent ESCs, P-bodies sequester RNAs linked to the totipotent 8-cell embryonic stage.

2/ Using fluorescence-activated particle sorting, we profiled P-body contents across developmental stages and vertebrate species. P-body composition is cell-type specific, and the sequestered transcripts are translationally repressed.

Selective RNA sequestration in biomolecular condensates directs cell fate transitions - Nature Biotechnology Stem cell differentiation is controlled by manipulating RNA condensates.

1/ Excited to share our new study with @brumbaugh-lab.bsky.social, out in @natbiotech.nature.com! P-bodies selectively sequester RNAs encoding cell fate regulators, often from the preceding developmental stage. Releasing these RNAs can drive changes in cell identity. 🧵 www.nature.com/articles/s41...

Check out ISSCR's society journal, Stem Cell Reports!

Excited to present at the CSHL Cell Fate Conversions 2025 meeting! Connect with me there to discuss whether your research is a fit for publishing in @stemcellreports.bsky.social. invt.io/1bxba296yhc #cshldirect

Check out ISSCR's society journal, Stem Cell Reports!

Excited to join the Stem Cell Reports team as an Early Career Editor! Grateful for the opportunity to contribute to the journal alongside fantastic colleagues. I encourage you to submit your work for consideration! @isscr.org @stemcellreports.bsky.social

Selective RNA sequestration in biomolecular condensates directs cell fate transitions Recent studies have emphasized the significance of biomolecular condensates in modulating gene expression through RNA processing and translational control. However, the functional roles of RNA condens...

New preprint from the lab! With @brumbaugh-lab.bsky.social , we show that P-bodies sequester developmentally relevant RNAs to influence cell fate. miRNAs direct this conserved regulatory process, and manipulating it enhances totipotency and germ cell programming. www.biorxiv.org/content/10.1...

Thrilled to share our new work on #aging in the murine hematopoietic system! There is a lot here- stem cell numbers, self-renewal rates, mutation rates, mutation signatures, clonal fitness, environmental effects...

H3K36 methylation regulates cell plasticity and regeneration in the intestinal epithelium Nature Cell Biology - Pashos et al. show that H3K36 methylation maintains intestinal epithelial fate commitment, whereas its suppression, which is also observed upon injury, induces a plastic state...

🥂Congrats to @brumbaugh-lab.bsky.social & co for their new study in @naturecellbiology.bsky.social showing that H3K36 methylation maintains #intestinal epithelial fate commitment and is involved in #regeneration.
rdcu.be/d8awu
www.nature.com/articles/s41...

Biomolecular condensates in immune cell fate - Nature Reviews Immunology Recent studies suggest that biomolecular condensates — membrane-less assemblies of proteins and nucleic acids — are involved in regulating gene expression to ensure proper immune cell development. Thi...

Review @natrevimmunol.bsky.social @distefanolab.bsky.social
Biomolecular condensates in immune cell fate
www.nature.com/articles/s41...

5/ Recent technological advancements have powered these studies, enabling answers to previously unanswerable questions (Fig. 4). Despite this progress, critical evaluation of the evidence concerning condensate properties is essential to understand their role in immune cell fate.

4/ Conversely, dysregulation of condensates has been linked to deleterious immune cell fates, including impaired function, autoimmunity, aging, and malignant transformation (Fig. 3).

3/ Recent studies have implicated condensates in the transcriptional and post-transcriptional processes that drive proper immune cell fates, from the level of hematopoietic stem cells to innate and adaptive immune cells (Fig. 2).

2/ Condensates are diverse, membraneless assemblies of nucleic acids and proteins (Fig. 1). They are thought to enable temporally precise control of gene expression by concentrating or segregating components of the gene regulatory machinery and their targets.

Biomolecular condensates in immune cell fate - Nature Reviews Immunology Recent studies suggest that biomolecular condensates — membrane-less assemblies of proteins and nucleic acids — are involved in regulating gene expression to ensure proper immune cell development. Thi...

1/ Pleased to share our review, published today
@natrevimmunol.bsky.social, highlighting how biomolecular condensates enhance the precision and flexibility of gene regulatory networks that guide fate decisions during normal and pathological immune cell development. www.nature.com/articles/s41...

Great thread from @levin-ferreyra.bsky.social about her latest work in the lab, out today in @emboreports.bsky.social ! 👇