Aritz Roa-Eguiara

Mapping bacteriophages SBGrid member Mihnea Bostina helps to uncover the first high-resolution, head-to-tail structural map of a Felixounavirus bacteriophage.

Mapping bacteriophages: a publication highlight from @keandreyam.bsky.social featuring a new article from the lab of Mihnea Bostina at the @universityofotago.bsky.social & colleagues.

Read more here: buff.ly/Wgfu3bt

#SBGrid #cryoem

Everyone interested in #virus loves a #bacteriophage right? It's amazing they are the most abundant and diverse biological entity on the planet, yet were discovered just over 100 years ago! To give them the attention we believe they deserve, npj Viruses has launched three collections on #phages:

Systematic mapping of bacteriophage gene essentiality with HIDEN‑SEQ The constant arms race of bacteriophages and their bacterial hosts has inspired major breakthroughs in biotechnology and shaped phages as fierce predators with great clinical potential to fight multid...

#microsky #phagesky

(Fancy!) transposon-insertion sequencing identifies essential genes in #phage. By @aharms485.bsky.social & Co.

www.biorxiv.org/content/10.1...

Congrats to @jimbeanbio.bsky.social for this very cool paper!

Cryo-EM structure of bacteriophage Bas63 reveals structural conservation and diversity in the Felixounavirus genus Cryo-EM of phage Bas63 reveals unique capsid and tail features, offering insights into Felixounavirus evolution and architecture.

Cryo-EM structure of bacteriophage Bas63 reveals structural conservation and diversity in the Felixounavirus genus | Science Advances www.science.org/doi/10.1126/...

Great manuscript and lovely thread to read, congrats!

Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states - Nature Communications Marín-Arraiza and colleagues report the cryo-EM structures of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states, providing structural insigh...

Thrilled to see Leyre’s PhD work finally out!

We reported the #cryoEM structures of an extracellular contractile injection system from P. luminescens in extended and contracted states, providing structural insight into its architecture and contraction mechanism 🔬
www.nature.com/articles/s41...

Awesome #phage #bioinformatics preprint from @shelbyeandersen.bsky.social, who is also very close to finishing her PhD and on the lookout for her next position 👀

Muyuan Chen has turned structural biology into an immersive experience with his new video game Meowtabolism, now available on Steam.
Try the demo here: store.steampowered.com/app/4045010/...
Give Muyuan feedback: steamcommunity.com/app/4045010
#ScienceGaming #StructuralBiology #CryoEM #STEMOutreach

Many of the most complex and useful functions in biology emerge at the scale of whole genomes.

Today, we share our preprint “Generative design of novel bacteriophages with genome language models”, where we validate the first, functional AI-generated genomes 🧵

SPEAR (bacterial nanosynringe) enables loading of different types of cargo (including folded ribonucleoproteins and single-stranded DNA) and targeting of defined cell types both in vitro and in vivo
www.nature.com/articles/s41...
@natbiotech.nature.com @zhangf.bsky.social

🚨 One week left to apply!
I am hiring a fully funded PhD fellow and a postdoc to work on membrane protein structural biology and pharmacology at Univ Copenhagen

Come work with a great team and exciting projects in a collaborative environment!

🔗 Links to the ads in comments

#CryoEM #AcademicJobs

I would say highly stable is still an understatement 👀👀 very cool!

Clear downgrade

Great resource! 😃

Faculty and academic positions – University of Copenhagen

Looking for a Post doc position in Structural Biology? We are hiring!
See post here:
jobbank.dk/job/2850337/...

The task is to continue our endeavor in solving cryo-EM structures of the human dopamine transporter. See: doi.org/10.1038/s415...
Thanks to the Novo Nordisk Foundation for funding.

The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation - Nature Microbiology Cryo-electron microscopy analysis of the extracellular flagellum structure, including the hook–filament junction and filament cap complex, reveals mechanisms of flagellin incorporation into the growin...

Very nice structure of the entire extracellular part of bacterial flagellum. Time to update my plant immunity teaching slides with the exact position of flg22 in the intact flagellum.
www.nature.com/articles/s41...

Postdoctoral Scientist - Structural Studies - Dr Sjors Scheres - LMB 2640 - Cambridgeshire job with MRC Laboratory of Molecular Biology (LMB) | 12840844 Postdoctoral Scientist Starting Salary: £41,344 Fixed term: 3 years MRC Laboratory of Molecular Biology, Cambridge, UK We are looking for a postdoc...

We have a 3-year funded postdoc position to work on structures of #amyloid filaments. If you are an expert in protein biochemistry, structural biology or #cryoEM / #cryoET, this could be for you! Feel free to email me with any questions. Come join us! 🤩

www.nature.com/naturecareer...

List of 30 CloneFISH cultures available for free (requester is asked to pay shipping fees).

Manuscript/resource alert #microsky 🦠 My lab offers the community a collection of 30 E. coli (CloneFISH) cultures, each carrying a plasmid for the heterologous expression of a (near) full-length 16S rRNA gene from one of 30 lineages of archaea, including 19 yet uncultured ones.

Running Phenix and stuck?
Now you can ask the new Phenix chatbot anything from the 600-page tutorial about how to run any program.
Big thanks to Tom Willinger for making this happen!
Try it out: phenix-online.org/version_docs...

RBPseg: Toward a complete phage tail fiber structure atlas RBPseg enables accurate modeling of tail fiber structure, providing the first comprehensive tail fiber structure atlas.

Excited to see our work published in @ScienceAdvances
!

We present RBPseg, a method for structural prediction of #phage receptor-binding proteins.

We applied it to diverse tail fibers and validated the predicted structures using #cryoEM.

Read here: www.science.org/doi/10.1126/...

RBPseg: Toward a complete phage tail fiber structure atlas pubmed.ncbi.nlm.nih.gov/40479047/ #cryoEM

SBGrid webinars are hosted with partial support from the NIH R25 Continuing Education for Structural Biology Mentors #GM151273, in collaboration with Co-PI J... CryoDRGN

In case you missed our May webinar, it is now available!! Ellen Zhong from Princeton University presented CryoDRGN-AI.

Topic: CryoDRGN-AI: Neural ab initio reconstruction of challenging cryo-EM and cryo-ET datasets
Host: Pete Meyer
Recorded on: May 20, 2025

buff.ly/jKjWTOa

#SBGridWebinars

The short version is: take the receptor or system you care about, make a strain with that knocked out or modified and a fluorophore, and the wildtype or different knockout with a different fluor. Look for differential plaquing. And it works!

Do you have any “published” E. coli phage? and willing to share for academic research?

most phages from yr 1980-2015 ish aren’t even available to researchers. they are either lost bcz of not sharing or archiving in registries.

I know I haven't been active on here much lately but...I am hiring a core facility postdoc to own the Krios, do a lot of cryoET jobs.rutgers.edu/postings/249...

Amazing work 🔥

Structural insights into allosteric mechanism of glycine transporter-mediated analgesia Chronic neuropathic pain, caused by nerve damage or disease, affects 10% of the population with rising incidence. The burden is exacerbated by ineffective treatments and overreliance on opioids. Neuro...

Beyond excited to present our study on the glycine transporter #GlyT2, a promising #non-opioid target for the treatment of neuropathic pain.
@biorxivpreprint.bsky.social

www.biorxiv.org/content/10.1...

Join the Blokesch lab (@EPFL) as a postdoc in Molecular Microbiology 🤩
Study Vibrio cholerae defense systems (phage/plasmid) in a dynamic, collaborative environment in Lausanne close to Lake Geneva in 🇨🇭!
Please visit my lab's webpage for details about the position and how to formally apply.

Structural characterization of an extracellular contractile injection system from Photorhabdus luminescens in extended and contracted states. Contractile injection systems (CISs) are phage-tail-like nanosyringes that mediate bacterial interactions by puncturing target cell membranes. Within these systems, Photorhabdus Virulence Cassettes (P...

🚨Super happy to see this preprint out!! Main part of Leyre’s PhD journey on the eCIS from Photorhabdus luminescens. Full structure using #cryoEM Check it out! 🎊

www.biorxiv.org/content/10.1...