Lutz Frönicke

"Expansion in situ sequencing" on the Aviti24 should be even wilder.

Metagenome sequencing: Soil is wild indeed.

Roche Highlights New Tests, Instrument Launches at Investor Day The firm touted new assays, provided an update on its nanopore sequencer, and offered insights into upcoming pipeline launches.

Roche has finally given their upcoming DNA sequencer a name - Axelios. So now we can stop calling it "Genia + Stratos".

www.genomeweb.com/molecular-di...

Focus your research on specific genes with #PacBio Targeted #HiFisequencing! Get cost-effective, scalable insights for tricky regions, accurate variants & phased haplotypes. Learn about PureTarget, HiFi Target Enrichment & PCR Amplicons.

Watch now: bit.ly/3Het9pD

#PrecisionWithHiFi

Now in Nature Methods: www.nature.com/articles/s41...

See @isidrolauscher.bsky.social's explainer bsky.app/profile/isid...

Evaluating the practical aspects and performance of commercial single-cell RNA sequencing technologies The rapid development of updated and new commercially available single-cell transcriptomics platforms provides users with a range of experimental options. Cost, sensitivity, throughput, flexibility, a...

A new comparative study of commercial single-cell RNAseq kits/chemistries from the Fred Hutchinson Cancer Center: They compared seven whole transcriptome kits and three TCR kits.
scRNA-seq
www.biorxiv.org/content/10.1...

a baby is eating a piece of food while sitting on the ground . ALT: a baby is eating a piece of food while sitting on the ground .

Trying a slightly new model (for us at least) of #openscience. With @ronanchaligne.bsky.social and @karolisk.bsky.social, we pitted 10x Genomics Chromium GEM-X against Illumina PIPseq V head-to-head to understand these two platforms for single-cell genomics. Some thoughts off the bleeding edge: /1

Great turnout at the "Stand Up For Science" event in Sacramento today.

These seem to be for DNA sequencing only. Since the native strand is not sequenced, it is unlikely that they will detect DNA base modifications.

Yay! Paper accepted!

2) AxBio will show off a long-read sequencer on February 5th-7th at a precision medicine conference in Santa Clara - supposedly enabling very cheap long-read sequencing:
www.axbio.com

Bold technologies in times of uncertain science funding.

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It looks like TWO nanopore sequencers will be announced next month.
Both are based on the "Genia" concept of sequencing labeled cDNA on silicon chip nanopores.

1) Roche promises ultra-high-throughput sequencing of short/medium-length samples at a webinar on February 20th: shorturl.at/9cjOG

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Bacterial single-cell RNA sequencing captures biofilm transcriptional heterogeneity and differential responses to immune pressure - Nature Communications Microbial biofilms contain subpopulations of cells that display metabolic and transcriptional diversity. Here, Korshoj & Kielian present an optimized bacterial single-cell RNA sequencing method an...

Microbial single-cell RNA-seq, now also for difficult samples:
"BaSSSh-seq captures extensive transcriptional heterogeneity during biofilm compared to planktonic growth."
Since the protocol uses split-pool barcoding, it can be relatively affordable.
www.nature.com/articles/s41...

Our lab's latest preprint investigates the dynamic RNA modification landscape in the #liverwort Riccia fluitans during its transition from a terrestrial to an aquatic environment. We employed @nanoporetech.com DRS for the identification and validation (adapted PRAISE metod) these modifications.

New method out for structural variants studies called Kanpig. Huge improvements on tandem repeat regions where 70% of sv are. Great new method from Adam English!

www.biorxiv.org/content/10.1...