Hao Yin

Really cool work here!! Congrats @emery-lab.bsky.social

Dynamic changes of TEADlight signal during myogenic differentiation. Dorsal (A) and front (C) views of a myotome and the overlaying dermomyotome, 48 h after electroporation of the TEADlight reporter (yellow) into the dorsomedial part of the somite. A plasmid driving the constitutive expression of mKate2-NLS (magenta) was used as an electroporation efficiency control (EP control). Image A is a maximal intensity projection of confocal images along the dorsoventral axis, while image C represents a 100 μm-thick virtual transection generated using the Imaris software. The primary myotome territory derived from the DML was arbitrarily divided into a medial half (mMy) and a lateral half (lMy) for quantification. B: Schematics illustrating the morphology of electroporated cells in images A and C. Electroporated cell nuclei are highlighted in magenta. D: Quantification of the TEADlight native signal relative to the mKate2-NLS signal. The TEADlight signal decreases as myofibers migrate laterally. A Friedman test on paired groups revealed significant differences between compartments (p = 0.0115). The insets above each point show TEADlight signal in representative cells.

#DBfeature 💡

The TEADlight reporter dynamics reflects variations in cell density and adhesion in vitro, providing a novel faithful read-out of YAP/TAZ-TEAD signaling.

By Valérie Morin, Yoann Le Toquin, Daria Tepordei, Christophe Marcelle, Emilie Delaune

tinyurl.com/4rhwjy4s

Promotional graphic announcing “AAV Packaged on Request – AAV9 now available!” with illustrated tubes and Addgene logo.

AAV9 is now available through Addgene's Packaged on Request service. With 4,000+ eligible cargo plasmids available, what would you like packaged in AAV9 for your next experiment?
Learn more: https://twp.ai/4ivYPg

Vascular Smooth Muscle Cell Metabolic Disorders & Aortic Aneurysm/Dissection

OXPHOS
ACO2 NR1D1 OGDH Pyruvate carboxylase

Glycolysis
PKM2, lactylation, ubiquitination

Hexosamine
PSMC1 O-GlcNAcylation ⬇️Proteasome

Sphingolipid
KLF9, GM3, ferroptosis

#Biomed_MDPI 2025
www.mdpi.com/2227-9059/13...

Soluble FGFR3 (extracellular domain dimer)-Fc
⏬fibroblast proliferation/contractility (FGF2, TGFβ)
vs Recifercept, a reference sFGFR3

⏬Ashcroft fibrosis score in 🐭🫁+Bleomycin
vs Alk5i SB-525334

A new avenue for #IdiopathicPulmonaryFibrosis🥸

#AJRCMB 2026
academic.oup.com/ajrcmb/advan...

🚨3-year Postdoc Position @halloulab.bsky.social (Kennedy Institute, Oxford) - on Spatial Biology & Bioinformatics of Fibrosis🚨

An exciting project combining #SpatialTranscriptomics & #Mechanobiology👇:

shorturl.at/LLS5r

Deadline: 16 March - Please RT 📢!

@kiroxford.bsky.social @ox.ac.uk

VEGF signaling is required for blastema growth and the effect of VEGF inhibition is reversible. (A) schematic of experimental design; (B) representative DMSO-treated limb at 11 dpa (scale bar 100 μm; arrowhead indicates blastema; dashed line indicates amputation plane); (C) representative AV951-treated limb at 11 dpa (scale bar 100 μm; arrowhead indicates blastema; dashed line indicates amputation plane); (D) AV951-treated blastemas are significantly smaller at 11dpa; (E) representative DMSO-treated limb at 22 dpa (scale bar 100 μm); (F) representative AV951-treated limb at 22 dpa (scale bar 100 μm; arrowhead indicates blastema); (G) AV951-treated limbs show no regeneration at 22 dpa; (H) skeletal prep of representative DMSO-treated limb at 22 dpa (scale bar 250 μm); (I) skeletal prep of representative AV95-treated limb at 22 dpa (scale bar 250 μm); (J) AV951-treated blastemas display blocked regeneration compared to controls; (K) AV951-treated blastemas can develop to palette stage upon treatment removal

#DBfeature

VEGF signalling promotes blastema growth and proliferation of both vascular and non-vascular cells during axolotl limb regeneration

By Aaron Savage et al.

tinyurl.com/3xs4nffs

This poses an intriguing question: how is cytoplasm robustly and accurately organized in absence of physical barriers, and how is cytoplasmic partitioning achieved within the constrained time window of rapid embryonic divisions? (2/9)

Really excited to share our new paper in @nature.com! We uncovered how a physical instability of the cytoplasm coupled with the cell cycle drives cytoplasmic partitioning in early embryos #zebrafish #drosophila. Read more in this🧵 www.nature.com/articles/s41... 🤩
@poldresden.bsky.social @mpi-cbg.de

Best Copilot ever 🫡

My Copilot is dog

#OsteoArthritis
InterMuscular Adipose Tissue from Vastus lateralis
6♀️case + 5♀️ctrl

Single-nuclei RNAseq IMAT▶️
Adipocyte, ~20% of non-myogenic cells
highest DEGs
⏫Leptin (➡️FAP/Fibroblast)
⏫Periostin, Ephrin A5 (➡️Mural cell)
⏬IL-16 (➡️Mφ)

#AgingCell 2025
onlinelibrary.wiley.com/doi/10.1111/...

#SciArt 🤝 #zebrafish 🟰 #ZebrafishZunday

🐟🎸 🧪

"Here, we present SPAtial Cell Exploration (SPACE), a spatial CRISPR screening platform that integrates whole-transcriptome profiling (~18,000 genes), multiplexed protein detection (~68 markers), and CRISPR perturbation mapping at subcellular resolution"

www.biorxiv.org/content/10.1...

Myeloid/Macrophage STING sustains Hepatic ischemia/reperfusion injury (70% warm ischemia)

STING IHC signal⬆️ in non-parenchymal liver cells in injured 🐭liver

Evidence from Lysm-Cre STING KO🐭 & STINGi (C-176, H-151)

#AJPathology 2022
www.sciencedirect.com/science/arti...

#CircularRNA #ChemoResistance

Circ76691 (1 of 6 cirRNAs⏫in #Oxaliplatin resistance #ColoRectalCancer in vitro, also⏫in vivo (N=11)) acts as a miR-589-3p sponge to relieve FGF9➡️⏬CRC apoptosis

Hi circ76691➡️⏬#DiseaseFreeSurvival (TCGA CRC)

#NoncodingRNARes 2025
www.sciencedirect.com/science/arti...

Even at times like that, when reason, science, medicine and human solidarity are under attack - there is hope - I had a great time visiting the super team at Tulane, giving two talks, seeing old friends, acquiring new ones - and discussing #CurePF4All
#ScienceMatters
##CureIPF
#AcademicNomads

Ablation of PKCα Phosphorylation by CRISPR-Cas9 Base Editing Rescues Heart Failure | Circulation Research BACKGROUND: The prevalence of heart failure is increasing globally, with poor prognosis, highlighting the need for novel therapeutic strategies. PKCα (protein kinase C alpha), encoded by PRKCA, plays ...

www.ahajournals.org/doi/10.1161/...

Deletion of Mfn2 in endothelial cells triggers a mitohormetic response that improves systemic metabolism and healthspan in mice Chivite et al. show that the genetic inactivation of the mitochondrial fusion protein MFN2 in the endothelium triggers a protective stress response that boosts fat burning, increases secretion of the ...

Nice collaboration with @grauperalab.bsky.social and Marc Claret! All credit to them.
www.cell.com/cell-metabol...

Sparcl1 mitigates abdominal aortic aneurysm through inhibiting lymphangiogenesis-mediated TLS formation - Nature Immunology Ruan and colleagues show that vascular resident macrophage-derived Sparcl1 has a protective effect during aorta aneurysm progression by interfering with FGF2-induced dysfunctional lymphangiogenesis, T...

Sparcl1 mitigates abdominal aortic aneurysm through inhibiting lymphangiogenesis-mediated tertiary lymphoid structure formation @natimmunol.nature.com
www.nature.com/articles/s41...

PDGF-D engages PDGFRβ in mice and NKp44 in humans to drive ILC3 responses @sciimmunology.bsky.social
www.science.org/doi/10.1126/...

One week from today--join us!

Final version @nature.com of our paper describing unconventional multicellular development in a choanoflagellate inhabiting an extreme environment. A ton of new data since the first @biorxivpreprint.bsky.social preprint (which we've kept updating).

A brief 🧵 (carried over from the old place)

MYH9 mutations differentially stabilize non-muscle myosin II filaments and induce distinct cellular aggregation phenotypes - Cellular and Molecular Life Sciences Cellular and Molecular Life Sciences - Mutations in the MYH9 gene, which encodes the heavy chain of the actin-based molecular motor non-muscle myosin II-A (NM2-A), cause a spectrum of rare blood...

link.springer.com/article/10.1...

ICYMI: Suggesting a potential therapy for these autism-related symptoms and intellectual disability…

This issue’s Cover features work by Hannelore Ehrenreich & team on the improvement of Primose syndrome traits in mice following erythropoietin treatment: doi.org/10.1172/jci....

#Zebrafish🐟 Neurodevelopment

scRNAseq 4, 7 dpf
Rohon-Beard sensory neuron (Spinal cord)

3 subtypes
kitb+ runx3+
calca+ tmem178+
adcyap1a+ prickle1a+

vs Trigeminal neuron (Head)

Fgf signaling▶️Axon maintenance & RB neuronal survival

#JNeurosci 2024 @SfNJournals
www.jneurosci.org/content/44/1...

My hand holding the glass showing the bottom is irregular and fits the wood below, which also has burnt marks on

And I absolutely love this terrarium, a surprise find from a local plant shop! Glass blown then pressed on wood while it’s still hot, so two of them fit perfectly

A network-based atlas of human skeletal muscle aging Skeletal muscle metabolic and physical capacities are influenced by both genetics and load status and decline with age. Recent advances in sequencing have detailed cell types at unprecedented detail; ...

A network-based atlas of human skeletal muscle aging
www.medrxiv.org/content/10.6...

I found this immune cell (🔴) prying open the junction between the ectoderm cells from underneath. You can see its protrusions probing (yellow arrow) and threading through between the other cells before the whole cell body is squeezed through in 1 sweeping motion. So cool! #FluorescenceFriday

Join us #NAVBO webinar with Dr. Sarah Sheppard, MD, Ph.D., Eunice Kennedy Shriver National Institute of Child Health and Human Development.
Title “Genomics informs precision medicine for vascular malformations." 🔥
The webinar is not recorded!
Register to attend members.navbo.org/ap/Events/Re...