Juan Pablo Tosar

It revealed postdocs are heterogeneous

mGem: Guides or triggers? Extracellular RNAs beyond vesicular miRNAs | mBio Blood and other extracellular biofluids have a high RNase content. Hence, from its origins, the exRNA field has focused on extracellular vesicles (EVs) as the main carriers of RNA molecules that move ...

With @amyhbuck we wrote this piece on extracellular RNAs that intends not to give answers but to propose questions that can expand the field in new directions.

Hope you like it! We really enjoyed a lot brainstorming it.

journals.asm.org/doi/10.1128/...

You can read the published version @pubs.acs.org here:

pubs.acs.org/doi/10.1021/...

Substantial hierarchical reductions of genetic and morphological traits in the evolution of rotiferan parasites Abstract. Within the last 800 million years animals evolved a vast range of diversity of species exhibiting an enormous disparity of forms and lifestyles.

🔥🔥🔥NEW PAPER OUT in @genomebiolevol.bsky.social
with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥
academic.oup.com/gbe/advance-...

Ribonuclease activity undermines immune sensing of naked extracellular RNA Castellano et al. show that when extracellular RNases are inhibited or absent, naked extracellular RNA is internalized spontaneously by cells, inducing inflammation mediated by endosomal TLRs or cytos...

Check out our paper about naked extracellular RNAs uptake, TLR signaling, endosomal escape, and the role of RNases in regulating this process.

www.cell.com/cell-genomic...

New preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm.

www.biorxiv.org/content/10.1...

There are concerns that Bluesky's impact has faded since the initial hype. This is false.

That impression results from reading headlines about social media instead of the hard data.

The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.

Plato’s allegory of the cave applied to small RNA sequencing and the nicked tRNA / tDR dilemma

Not all tRNA fragments (tDRs) are created equal. Here we share methods to differentiate:

1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.

1) www.sciencedirect.com/science/arti...

2) www.sciencedirect.com/science/arti...

Graphical abstract showing Indian rhesus macaque with radiolabeled lipid nanoparticles injected into the right deltoid and major lymphatic organization of the macaque therein.

Here's a clever new study looking at where mRNA vaccine lipid nanoparticles (mRNA-LNPs) go after vaccination (using relevant doses of vaccine) in non-human primates (and mice): www.cell.com/molecular-th...

Brief thread🧵

GRC on RNA Nanotechnology came to an end yesterday. Thanks to the organizers for inviting me to present our work on the uptake of naked exRNAs, and to serve as discussion leader of the RNA Delivery session. What a fantastic and enjoyable conference!
www.grc.org/rna-nanotech...

Protocol for isolating RNA from the surfaces and apoplastic spaces of plant leaves.

Very happy to start the new year with publication of our new work on leaf surface RNA. Plant leaves secrete diverse RNAs that are surprisingly stable on their surfaces. Next question to answer: What do they do? www.pnas.org/doi/epub/10....

Why Bax detection in >1400 publications might be flawed - Cell Death & Disease Cell Death & Disease - Why Bax detection in &gt;1400 publications might be flawed

"Here, we show that this Ab appears to be unreliable due to providing false-positive signals at the expected molecular weight of Bax in immunoblotting experiments and likewise provides false-positive signals in immunofluorescence-based detection of Bax expression"

www.nature.com/articles/s41...

Some of you probably do not realize how difficult it is to get a BIG international grant when located in LMIC (especially in Argentina). I am so proud of you @pstrobl.bsky.social. So well deserved. I am so happy to be part. You are the best!! @wellcometrust.bsky.social @unsamoficial.bsky.social

But please dont share this as a mantra for success. If your success comes at the direct expense of your colleagues because they are doing all the work everyone is supposed to share in, it does not make you ‘successful’ it just makes you a horrible colleague.

Also see peer review

Rant over. For now

This will be the next level of scientific devaluation: science to proof a political standpoint, rather than policy following scientific proof.

Thus, endosomal signaling within a tissue microenvironment is likely, while miRNA signaling between cells from different organs would require highly efficient release and/or highly efficient uptake. Both are extracellular RNA-mediated intercellular communication processes. But likelihood differs

I guess it depends on several factors. First, what we understand by function. miRNAs require the same sequence to be present in the cytosol at a certain amount. Endosomal TLR signaling is less sequence-dependent. Second, the conc. might be high next to the releasing cell, then decays (cont…)

The Gartner Hype cycle

As a relatively new field, it’s following this path. With the exception that, depending who you talk to, we might still be in the peak of inflated expectations, the trough of disillusionment or the slope of enlightenment. There are lots of artifacts going on, but probably also important biology

MirGeneDB 3.0: improved taxonomic sampling, uniform nomenclature of novel conserved microRNA families and updated covariance models Abstract. We present a major update of MirGeneDB (3.0), the manually curated animal microRNA gene database. Beyond moving to a new server and the creation

MirGeneDB 3.0 is published.
Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too

academic.oup.com/nar/advance-...

Frontiers | Serum-derived host miR-423-5p and miR-378a-3p as molecular markers for severe tuberculosis: a promising prognostic tool for survival Tuberculosis (TB) remains a leading cause of infectious disease-related mortality. Annually, 10 million people contract TB, resulting in 1.5 million deaths, ...

First post, last paper out www.frontiersin.org/journals/tub... @carlos-robello.bsky.social

#RNAsky #RNAbiology

The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1 Gene expression programs underpin the development of shared phenotypes, yet the importance of transc

The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1

📢SAVE THE DATE📢

The 4th Molecular Biosystems Conference is set!

Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️

Stay tuned for more details!

🗓️Sep 29-Oct 3, 2025
📍Puerto Varas, Chile
🌐http://molbiosystems.com